We narrowed to 7,534 results for: ski

1. pAAV.hSynapsin.SF-iGluSnFR.A184V

   Plasmid

   #106175

   Purpose

   Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.A184V
   

   Use

   AAV

   Mutation

   GltI: A184V

   Promoter

   hSynapsin

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-TRE-DIO-Casp3-TEV

   Plasmid

   #183766

   Purpose

   This plasmid is for use with neuronal cell-type selective activity tagging. The Caspase3 expression requires both neural activity and Cre recombinase.

   Depositor

   William Wisden

   Article

   Yu et al Science. 2022 Jul;377(6601):63-72. doi:

   Insert

   AAV transgene - tetO promoter-flex/DIO-Caspase3-TEV
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Expression

   Mammalian

   Promoter

   tetO promoter

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.CAG.SF-iGluSnFR.S72A

   Plasmid

   #106200

   Purpose

   Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.S72A
   

   Use

   AAV

   Mutation

   GltI: S72A

   Promoter

   CAG

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-flex-GFP-shRNA-GluN1

   Plasmid

   #182502

   Purpose

   Expresses shRNA against mouse NMDA receptor NR1 subunit. Flexed cassette driven by hdc (pan neuronal) gene promoter

   Depositor

   William Wisden

   Article

   Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

   Insert

   pan neuronal promoter (hdc basal promoter) driving flexed-GFP-mir30-shRNA NR1 cassette
   

   Use

   AAV, Cre/Lox, and RNAi

   Expression

   Mammalian

   Promoter

   fragment of histidine decarboxylase gene promoter…

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1/NDUFS2/myc-His

   Plasmid

   #127486

   Purpose

   Expresses myc-tagged NDUFS2 in mammalian cells

   Depositor

   Shashi Jain, Dieter Wolf

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS2 (NDUFS2 Human)
   

   Tags

   myc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSG5-hEKLF

   Plasmid

   #67835

   Purpose

   Expression of human EKLF driven by SV40 promoter for use in activation studies

   Depositor

   James Bieker

   Article

   Mas et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

   Insert

   EKLF (KLF1 Human)
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pLKO.1-shLuc.mKO2

   Plasmid

   #85224

   Purpose

   shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   Firefly Luciferase
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNL-CEF-IgA1-IRES-GFP

   Plasmid

   #187007

   Purpose

   Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1

   Depositor

   Hermann Eibel

   Article

   Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

   Insert

   Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1
   

   Use

   Lentiviral

   Available Since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.GFAP.SF-iGluSnFR.A184S

   Plasmid

   #106192

   Purpose

   Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Has Service

   AAV1

   Insert

   SF-iGluSnFR.A184S
   

   Use

   AAV

   Mutation

   GltI: A184S

   Promoter

   GFAP

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. HiBiT-FZD6

    Plasmid

    #195844

    Purpose

    Expresses human FZD6 with an N-terminal HiBiT; contains M345L

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD6 (FZD6 Human)
    

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    M345L

    Promoter

    CMV

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPN089

    Plasmid

    #91614

    Purpose

    Express sgRNA targeting human FURIN

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    FURIN (FURIN Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pYTSK01K_0G7

    Plasmid

    #177296

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR

    Depositor

    Karl-Erich Jaeger

    Article

    Tiso et al Front Bioeng Biotechnol. 2020 Aug 20;8:9

    Insert

    PaacC1-aacC1
    

    Use

    Synthetic Biology; Yeast expression, tn7 genomic …

    Expression

    Bacterial

    Available Since

    Aug. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1/NDUFS7/myc-His

    Plasmid

    #127488

    Purpose

    Expresses myc-tagged NDUFS7 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS7 (NDUFS7 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-myr-AKT-HA

    Plasmid

    #31790

    Depositor

    Xin Chen

    Article

    Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Entry vector

    Tags

    HA and Myr

    Available Since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

15. His-FLAG-Tev_ACTA1 (alpha-actin)

    Plasmid

    #188452

    Purpose

    Express in mammalian cells human alpha-actin (ACTA1) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.

    Depositor

    Roberto Dominguez

    Article

    Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

    Insert

    ACTA1 (ACTA1 Human)
    

    Tags

    6xHis-FLAG

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-EF1a-IRES-Puro PFKM

    Plasmid

    #201638

    Purpose

    Over-expression of untagged human PFKM

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    PFKM (PFKM Human)
    

    Use

    Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TurboID-13x-EV

    Plasmid

    #215086

    Purpose

    Expresses TurboID-13x[linker] in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    TurboID (birA )
    

    Use

    Retroviral

    Tags

    13x [linker]

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PL-5LTR-GW-A

    Plasmid

    #138480

    Purpose

    For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
    

    Expression

    Mammalian

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCEP4-FLAG-GRM3-VN

    Plasmid

    #98965

    Purpose

    FLAG-tagged human mGluR3 fused to N-terminus of split Venus

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    GRM3 (GRM3 Human)
    

    Tags

    FLAG (dykddddk) epitope tag, Signal/leader sequen…

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5-FRT-TO-Nsp13_GC3opt

    Plasmid

    #157714

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Available Since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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