We narrowed to 11,231 results for: SHA

1. pFUW-tetO-Irf8

   Plasmid

   #139838

   Purpose

   doxycycline-inducible expression of mouse Irf8 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Irf8 (Irf8 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-mCMV

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-miRFP670nano3-Blast-H3C2

   Plasmid

   #207784

   Purpose

   Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H3C2 Homology Arms flanking a miRFP670nano3-Blast Cassette (H3C2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pMK265 (RAD21-mAC Hygro)

   Plasmid

   #140539

   Purpose

   RAD21 tagging with mAID-Clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   RAD21 (RAD21 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC-MLXγ

   Plasmid

   #211755

   Purpose

   Cloning vector for the expression of mouse MLXγ

   Depositor

   Russell Goodman

   Article

   Singh et al Cell Metab. 2024 Jan 2;36(1):144-158.e7.

   Insert

   MLX (Mlx Mouse)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. mAID-BRD4 donor

   Plasmid

   #140650

   Purpose

   BRD4 tagging with mAID

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   BRD4 (BRD4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-sfCherry11x4-ß-actin

   Plasmid

   #70220

   Purpose

   Expresses sfCherry11x4-ß-actin in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   sfCherry11x4-actin (ACTB Human)
   

   Use

   Tags

   sfCherry11x4

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-P2A_BAF_G47E

   Plasmid

   #101774

   Purpose

   Expresses mutant BAF (G47E) in human cells (with EGFP produced from the same transcript as expression control)

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP-P2A

   Expression

   Mammalian

   Mutation

   G47E mutation

   Promoter

   EF1a

   Available since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. plentiCRISPR-v2 Puro EIF2AK3/PERK_sgRNA

   Plasmid

   #218666

   Purpose

   sgRNA targeting human EIF2AK3/PERK

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   EIF2AK3 (EIF2AK3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EGFP-P2A_BAF

   Plasmid

   #101773

   Purpose

   Expresses BAF in human cells (with EGFP produced from the same transcript as expression control)

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 TAK1/F

    Plasmid

    #44161

    Depositor

    Xin Lin

    Article

    Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

    Insert

    Tak1 (Map3k7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)

    Plasmid

    #165456

    Purpose

    Inducible ZEB2 expression from AAVS1 locus

    Depositor

    Madeline Lancaster

    Article

    Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

    Insert

    ZEB2 (ZEB2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiGuide-Crimson

    Plasmid

    #70683

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.

    Depositor

    Daniel Bauer

    Article

    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

    Inserts

    S. pyogenes sgRNA cassette
    Crimson Reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-a and hU6

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2_IRES2_CyOFP1_KCNQ2 var4

    Plasmid

    #173155

    Purpose

    For mammalian expression of KCNQ2 and creating variants

    Depositor

    Al George

    Article

    Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

    Insert

    KCNQ2 (KCNQ2 Human)
    

    Use

    Tags

    IRES2-CyOFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Simian CMV IE94

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUW-tetO-RUNX1

    Plasmid

    #139821

    Purpose

    doxycycline-inducible expression of Human RUNX1 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Runx1 (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EGFP-P2A_BAF_L58R

    Plasmid

    #101775

    Purpose

    Expresses mutant BAF (L58R) in human cells (with EGFP produced from the same transcript as expression control)

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP-P2A

    Expression

    Mammalian

    Mutation

    L58R mutation

    Promoter

    EF1a

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. BTG1

    Plasmid

    #155425

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    BTG1 (BTG1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. qTAG-C-Solo-mStayGold-VIM

    Plasmid

    #227302

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the VIM locus. For intermediate filament visualization. To be co-transfected with sgRNA plasmid px330-PITCh-VIM (Addgene #227301)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    VIM Homology Arms flanking a mStayGold Tag (VIM Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV[Exp]-Puro-EF-1α>TurboRFP(ns):T2A:{H2B-RFP}

    Plasmid

    #201120

    Purpose

    RFP expression in cells under EF-1α promoter; Histone-bound, nuclear

    Depositor

    Ori Bar-Nur

    Article

    Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

    Insert

    TurboRFP; H2B-RFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    No

    Promoter

    Available since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV V5-lamin A

    Plasmid

    #175107

    Purpose

    Lentiviral expression of V5-tagged mouse Lmna

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Lmna (Lmna Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. plentiCRISPR-v2 Puro EIF2AK4/GCN2_sgRNA

    Plasmid

    #218528

    Purpose

    sgRNA targeting human EIF2AK4/GCN2

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK4 (EIF2AK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLV-CDH5-mRuby3-IRES-puro

    Plasmid

    #85144

    Purpose

    lentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    CDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3 (CDH5 Human)
    

    Use

    Lentiviral

    Tags

    mRuby3

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SunTag-FWAgRNA-4-22aa-TET1cd

    Plasmid

    #106435

    Purpose

    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pet16b-Avi-ubiquitin_rbs_BirA

    Plasmid

    #134897

    Purpose

    Expresses HIS-Avi-tagged ubiquitin for recombinant protein production

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Inserts

    Ubiquitin (UBB Human)
    BirA
    

    Use

    Tags

    10HIS-AviTag-PreScission(3C)protease

    Expression

    Bacterial

    Mutation

    Promoter

    RBS and T7

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRRLNeo-pEF1a-p53ash-mCerulean

    Plasmid

    #69579

    Purpose

    Expresses p53 tagged with mCerulean

    Depositor

    Galit Lahav

    Article

    Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

    Insert

    p53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    7 silent mutations in p53 DNA to prevent targetin…

    Promoter

    EF1alpha

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CTCF-C CRISPR

    Plasmid

    #140647

    Purpose

    CTCF tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CTCF (CTCF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EGFPC2-CB2 SH3-

    Plasmid

    #68822

    Purpose

    EGFP tagged Collybistin expression in mammalian cells

    Depositor

    Shiva Tyagarajan

    Article

    de Groot et al PLoS Genet. 2017 Oct 25;13(10):e1007073.

    Insert

    Collybistin (Arhgef9 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SYKA-c020

    Plasmid

    #175489

    Purpose

    Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.

    Depositor

    Opher Gileadi

    Article

    Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

    Insert

    SYK (SYK Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pB-CAGGS-dCas9

    Plasmid

    #110823

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCF140_Gag-SpyCas9-NLS

    Plasmid

    #225959

    Purpose

    CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    CMV-Intron-Gag-SpyCas9-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMSCV-mLin28A

    Plasmid

    #26357

    Depositor

    George Daley

    Article

    Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

    Insert

    lin-28 homolog A (Lin28a Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only