We narrowed to 6,934 results for: CAD

1. GFP-CYLD-C601A

   Plasmid

   #60028

   Purpose

   N-terminal GFP tagged catalytic inactive (C601A) expression

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   Cylindromatosis (CYLD Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Cysteine 601 changed to Alanine

   Promoter

   CMV

   Available since

   Nov. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRE-Tight-FLAG-CYLD-C601A

   Plasmid

   #60029

   Purpose

   CYLD (C601A) catalytic dead under control of Tet promoter with N-terminal FLAG tag

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   Cylindromatosis (CYLD Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Cysteine 601 changed to Alanine

   Promoter

   TRE

   Available since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

3. Flag- mCry1ER-pBABEpuro

   Plasmid

   #61429

   Purpose

   Express mCRY1-ER* fusion protein

   Depositor

   Aziz Sancar

   Article

   Ye et al Genes Dev. 2014 Sep 15;28(18):1989-98. d

   Insert

   mCry1 (Cry1 Mouse)
   

   Use

   Retroviral

   Tags

   Flag and mouse estrogen receptor ligand binding d…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pRetroX-Tight-Pur-Trim69 isoform B (codon optimized)

   Plasmid

   #199567

   Purpose

   Stable and dox. inducible expression of Trim69 isoform B (codon optimized) after retroviral-mediated gene transfer

   Depositor

   Andrea Cimarelli, Yuxin Song

   Article

   Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

   Insert

   Trim69 isoform B (codon optimized) (TRIM69 Human)
   

   Use

   Retroviral; Allows for puromycin selection

   Tags

   Flag

   Expression

   Mutation

   codon optimized

   Promoter

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FLAG-LplA(AAG)

   Plasmid

   #61307

   Purpose

   Contains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expression

   Depositor

   Alice Ting

   Article

   Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

   Insert

   E. coli lipoic acid ligase
   

   Use

   Tags

   6xHis, Flag, T7 tag, and Xpress epitope

   Expression

   Mammalian

   Mutation

   E20A, F147A, H149G

   Promoter

   CMV

   Available since

   March 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIneo-lambdaN-HA-HsDCP1a_G

   Plasmid

   #146400

   Purpose

   Mammalian Expression of HsDcp1a

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   HsDcp1a (DCP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   two silent mutations T237C and G1716T compared to…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-6p-hDcp2 E147/8Q

   Plasmid

   #72217

   Purpose

   bacterial expression of GST-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

   Depositor

   Megerditch Kiledjian

   Article

   Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

   Insert

   Dcp2 (DCP2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   E147Q, E148Q and F404L (please see depositor'…

   Promoter

   tac

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-TRAIL-R3

   Plasmid

   #61384

   Purpose

   Expresses TRAIL receptor 3

   Depositor

   Francis Chan

   Article

   Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

   Insert

   TRAIL receptor 3 (TNFRSF10C Human)
   

   Use

   Tags

   HA tag and TNF receptor leader peptide

   Expression

   Mammalian

   Mutation

   TRAIL-R3 leader peptide (aa1-29) replaced with TN…

   Promoter

   Available since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-short Cre-on G/W

   Plasmid

   #86949

   Purpose

   Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. For introducing large constructs into AAV

   Depositor

   Rob Mitra, Joseph Dougherty

   Article

   Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. (p)odr-3::GFP::EGL-4

    Plasmid

    #100897

    Purpose

    GFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.

    Depositor

    Noelle L'Etoile

    Article

    Lee et al Proc Natl Acad Sci U S A. 2010 Mar 30;10

    Insert

    56-2741: odr-3 promoter
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    odr-3

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28_6xHis-PARP1-Y986H

    Plasmid

    #174801

    Purpose

    Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Y986H (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y986H, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28_6xHis-PARP1-Y986S

    Plasmid

    #174802

    Purpose

    Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Y986S (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y986H, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28_6xHis-PARP1-G972R

    Plasmid

    #174800

    Purpose

    Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-G972R (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    G972R, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag‐mTim1 (Bl/6)

    Plasmid

    #49205

    Purpose

    expresses Flag tagged mouse Tim1

    Depositor

    Lawrence Kane

    Article

    de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

    Insert

    Tim1 (BL/6 allele) (Havcr1 Mouse)
    

    Use

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    Tim1 lacks its own signal sequence

    Promoter

    EF1a

    Available since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

15. 3611 pcDNA3 Bim

    Plasmid

    #8804

    Depositor

    Stanley Korsmeyer

    Article

    Harada et al Proc Natl Acad Sci U S A 2004 Oct 26;101

    Insert

    Bim (Bcl2l11 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pcdna DSG-2 TS

    Plasmid

    #89465

    Purpose

    DSG-2 tension sensor, using TSmod inserted in human DSG-2

    Depositor

    Daniel Conway

    Article

    Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

    Insert

    desmoglein-2 (DSG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBV-Luc/Del-6

    Plasmid

    #14969

    Depositor

    Joan Massague

    Article

    Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

    Insert

    c-myc promoter (-109/+334) (MYC Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    -109/+334 (relative to the P2 transcription initi…

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pLV-mScarlet-SARS-CoV-2-5'UTRΔSL1

    Plasmid

    #191484

    Purpose

    Lentiviral expression of SARS-CoV-2 5' UTR ΔSL1

    Depositor

    Hao Wu

    Article

    Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

    Insert

    SARS-CoV-2 5' UTR ΔSL1 (NEWENTRY Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. mEos3-Rac1

    Plasmid

    #92039

    Purpose

    Expresses mEos3-Rac1 fusion protein

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Use

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. SunTagCACTA1g2-22aa-TET1cd

    Plasmid

    #106437

    Purpose

    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoter

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    CACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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