We narrowed to 9,193 results for: Pol;

1. pCellFree_G03 HDAC1

   Plasmid

   #67101

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   HDAC1 (HDAC1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. p21 promoter E

   Plasmid

   #16466

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

   Insert

   p21 promoter (CDKN1A Human)
   

   Expression

   Mammalian

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

3. Flag-ATXN1_V591A_S602D

   Plasmid

   #48190

   Purpose

   Expresses V591A and S602D mutated Ataxin1

   Depositor

   Huda Zoghbi

   Article

   Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

   Insert

   Ataxin1 (ATXN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Flag-ATXN1_V591A_L686E

   Plasmid

   #48191

   Purpose

   Expresses V591A and L686E mutated Ataxin1

   Depositor

   Huda Zoghbi

   Article

   Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

   Insert

   Ataxin1 (ATXN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Flag-ATXN1_S602D_L686E

   Plasmid

   #48192

   Purpose

   Expresses S602D and L686E mutated Ataxin1

   Depositor

   Huda Zoghbi

   Article

   Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

   Insert

   Ataxin1 (ATXN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p5A0-T

   Plasmid

   #92016

   Purpose

   Expresses TALE targeted to lac operator without TEV cut sites, anhydrotetracycline inducible TEV protease

   Depositor

   Brian Pfleger

   Article

   Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

   Inserts

   TALE targeting lac operator
   Tobacco Etch Virus Protease
   

   Use

   TALEN

   Tags

   5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

   Mutation

   S219V

   Promoter

   J23102 Promoter (from Anderson promoter library) …

   Available Since

   Jan. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pY71-tet.I.8

   Plasmid

   #133521

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 11 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP ratAPOBEC1 L173A/G227A

   Plasmid

   #167169

   Purpose

   Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.

   Depositor

   Silvestro Conticello

   Article

   Chieca et al bioRxiv 2021

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Expression

   Mammalian

   Mutation

   changed Leucine 173 to Alanine; changed Glycine 2…

   Promoter

   CMV promoter

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIC389

   Plasmid

   #37387

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

   Insert

   LGN (GPSM2 Human)
   

   Use

   Retroviral

   Tags

   GFP, S-tag, and TEV

   Expression

   Mammalian

   Mutation

   deleted amino acid 1-364 and 524

   Available Since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA pDEST40 Cas9 GW STOP

    Plasmid

    #183196

    Purpose

    Destination vector

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCellFree_G03 DDX5

    Plasmid

    #67111

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pXylA-RgTALsyn

    Plasmid

    #100948

    Purpose

    Expression of Tyrosine Ammonia Lyase from constitutive XylA promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    XylA (Constitutive)

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pECFP-Cdc42-Y40C

    Plasmid

    #105295

    Purpose

    Control for FRET biosensor analysis - cannot bind downstream effectors

    Depositor

    Kenneth Yamada

    Article

    Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

    Insert

    Cdc42 (CDC42 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Y40C

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. secCitrine

    Plasmid

    #181964

    Purpose

    Basic chitinase signal peptide fused to Citrine, under control of MUM4_1.5kb promoter

    Depositor

    George Haughn

    Article

    Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

    Insert

    signal peptide sequence from basic chitinase
    

    Tags

    Citrine YFP

    Expression

    Plant

    Promoter

    1.5 kb MUM4 promoter

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GAL FLAG46QDProCFPp303

    Plasmid

    #15585

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 46Q Delta Pro (HTT Human)
    

    Tags

    CFP and FLAG

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pCellFree_G03 CTTN

    Plasmid

    #67100

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CTTN (CTTN Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. GPD 25QDProGFP p416

    Plasmid

    #15568

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 25Q Delta Pro (HTT Human)
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pEGFP_C1 I3

    Plasmid

    #61397

    Purpose

    Expresses GFP-tagged Inhibitor-3 in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

    Insert

    Inhibitor-3 (PPP1R11 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pGEM-myr-eGFP-SV40pA-FKF

    Plasmid

    #119866

    Purpose

    Donor cassette containing myr-tagged eGFP and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

    Insert

    myr-tagged eGFP and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Tags

    2x myristoylation

    Expression

    Bacterial

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p21 promoter C

    Plasmid

    #16464

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 promoter (CDKN1A Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only