164,802 results

1. pDendra2-H2b

   Plasmid

   #157817

   Purpose

   Fluorescent reporter for histone H2B tracking

   Depositor

   Xin Chen

   Article

   Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

   Insert

   Histone H2B (H2bc3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-loxpSTOPloxp-lyn-NeonGreen

   Plasmid

   #175257

   Purpose

   membrane target protein labelling

   Depositor

   Frank Bradke, Sebastián Dupraz

   Article

   Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

   Insert

   Lyn membrane targeting motif
   

   Use

   Cre/Lox

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   pCAG

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

   Plasmid

   #139997

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-PE2-SpG

   Plasmid

   #159978

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-SpG
   

   Use

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSKA413

   Plasmid

   #80381

   Purpose

   Constitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.

   Depositor

   Mustafa Khammash

   Article

   Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

   Inserts

   ccaS (complement)
   ccaR (complement)
   gfpmut3
   

   Use

   Synthetic Biology; Optogenetics

   Tags

   FLAG tag

   Expression

   Bacterial

   Mutation

   Promoter

   ccaR and cpcG2Δ59

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-mCh-Cry2WT

   Plasmid

   #101221

   Purpose

   Blue-light dependent optogenetic protein Cry2

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

   Insert

   Cry2PHR
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNK497

   Plasmid

   #219754

   Purpose

   MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1-MKLP1

   Plasmid

   #70145

   Purpose

   mammalian expression of MKLP1 with an eGFP fusion

   Depositor

   Masanori Mishima

   Article

   Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

   Insert

   MKLP1 (KIF23 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9

   Plasmid

   #84242

   Purpose

   Expresses GA-inducible KRAB-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sp dCas9
   GID1-KRAB
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. DJ-1 pET15b

    Plasmid

    #60678

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

    Insert

    DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

11. iRFP670-KASH2

    Plasmid

    #187021

    Purpose

    Inducible disruption of LINC Complex

    Depositor

    Jan Lammerding

    Insert

    KASH2
    

    Use

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. moxGFP

    Plasmid

    #68070

    Purpose

    C-terminal fusion to moxGFP

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    moxGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1 Blast NT shRNA

    Plasmid

    #123306

    Purpose

    Expression of a non-targeting shRNA

    Depositor

    Giovanni Tonon

    Article

    Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

    Insert

    Non-targeting shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX304 CMV OMM-FKBP-V5-sTurboID (N)

    Plasmid

    #153006

    Purpose

    Expresses split-TurboID N-terminal fragment targeted to outer mitochondrial membrane

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    OMM (Tom20)-sTurboID (N) (L73)
    

    Use

    Lentiviral

    Tags

    FKBP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 VDAC1-TC

    Plasmid

    #211733

    Purpose

    Expresses tetracysteine tagged VDAC1 in mammalian cells. TC-tag is attached to the C-terminus of VDAC1.

    Depositor

    Roland Malli

    Article

    Pilic et al bioRxiv 2023.09.08.556841

    Insert

    Voltage-dependent anion channel 1 (VDAC1 )
    

    Use

    Tags

    Tetracysteine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDA040.BLD7-mRFP

    Plasmid

    #206800

    Purpose

    EL222_D7_mRFP

    Depositor

    James Carothers

    Article

    Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

    Insert

    BLD7.mRFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCG_IFITM3

    Plasmid

    #179957

    Purpose

    Expression vector for human IFITM3.

    Depositor

    Frank Kirchhoff, Konstantin Sparrer

    Article

    Prelli Bozzo et al Nat Commun. 2021 Jul 28;12(1):4584.

    Insert

    IFITM3 (IFITM3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLIX403_UBAP2L_mCherry

    Plasmid

    #105286

    Purpose

    puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein

    Depositor

    Eugene Yeo

    Article

    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

    Insert

    UBAP2L (UBAP2L Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti X1 Puro empty (w610-1)

    Plasmid

    #20953

    Purpose

    3rd gen lentiviral empty control vector, Puro

    Depositor

    Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMD2.G-K47Q/R354Q

    Plasmid

    #207323

    Purpose

    VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    VSV-G (VSIVgp4 Vesicular Stomatitis Virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K47Q/R354Q

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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