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169,691 results

Showing: 5101 - 5120 of 169691 results

  2. TFORF1057

    Plasmid
    #143912
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFATC1 (NFATC1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143912-plasmid-map-sequence-id-404578

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  4. pBad-CAts TAG97

    Plasmid
    #105668
    Purpose
    expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 97 and C-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 97
    Promoter
    pBad/arabinose
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105668-plasmid-map-sequence-id-205515

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  6. eIF2a 2

    Plasmid
    #21808
    Depositor
    David Ron
    Insert
    eIF2a (EIF2S1 Human)
    Expression
    Mammalian
    Mutation
    S52A
    Available Since
    Sept. 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    21808-plasmid-map-sequence-id-229779

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  13. pEVOL_PylRS(AF)-tRNA

    Plasmid
    #223511
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-
    Inserts
    Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Y306A; Y384F
    Promoter
    araBAD, rrnB, and glnS and proK
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223511-plasmid-map-sequence-id-447439

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  16. ADORA3-Tango

    Plasmid
    #66212
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADORA3 (ADORA3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66212-plasmid-map-sequence-id-485939

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  20. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    61427-plasmid-map-sequence-id-322387

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Showing: 5101 - 5120 of 169691 results