159,557 results

1. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

   Plasmid

   #139997

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. BIN1-pmCherryN1

   Plasmid

   #27693

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   BIN1 (Bin1 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pNP1950

   Plasmid

   #222729

   Purpose

   J23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. ampkar

   Plasmid

   #35097

   Purpose

   A fluorescent reporter of AMPK activity and cellular energy stress

   Depositor

   Lewis Cantley

   Article

   Tsou et al Cell Metab. 2011 Apr 6;13(4):476-86.

   Insert

   ampkar
   

   Use

   Tags

   ECFP and Venus

   Expression

   Bacterial

   Mutation

   na

   Promoter

   CMV

   Available since

   March 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLPT107

   Plasmid

   #85525

   Purpose

   Repressilator with triple reporter

   Depositor

   Johan Paulsson

   Article

   Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

   Inserts

   mCfp
   mVenus
   tetR
   lacI
   cI
   mKate2
   

   Use

   Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-mCherry

   Plasmid

   #41583

   Depositor

   Phil Sharp

   Article

   Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pORANGE GFP-Camk2a KI

   Plasmid

   #131484

   Purpose

   Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Camk2a, Camk2a Rat, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Arp2/3_pBIG2abcd

   Plasmid

   #173683

   Purpose

   Insect expression of the 7-subunit Human Arp2/3 complex

   Depositor

   Roberto Dominguez

   Article

   Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

   Inserts

   Arp2
   Arp3
   ArpC2
   ArpC3-FLAG
   ArpC4
   ArpC5
   ArpC1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW57.1_blasti

   Plasmid

   #194067

   Purpose

   3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORTMAGE-3

    Plasmid

    #72678

    Purpose

    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.

    Depositor

    Csaba Pál

    Article

    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

    Insert

    mutL E32K
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    E32K mutation conferring dominant mutator phenoty…

    Promoter

    Available since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. T7_CC_Vpx-SIV_WPRE_IVT_Template

    Plasmid

    #216792

    Purpose

    Template for in vitro transcription of Vpx (SIV).

    Depositor

    Daniel Bauer

    Article

    Levesque et al Nat Biotechnol. 2024 May 28. doi: 10.103

    Insert

    Vpx (SIV)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSCAR_Cas9-blast_GFP

    Plasmid

    #162074

    Purpose

    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Inserts

    Cas9-2a-blasticidin S deaminase
    EGFP
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in M. musculus

    Promoter

    EF1a and sv40

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. ALIX-mNeonGreen

    Plasmid

    #191191

    Purpose

    Full-length ALIX with C-terminal mNeonGreen tag.

    Depositor

    Jin Zhang

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    ALIX (PDCD6IP Synthetic, Human)
    

    Use

    Tags

    6xHIS and mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. attP-puro donor

    Plasmid

    #182139

    Purpose

    attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attP-EGFP-EF1α-PuroR-T2A-TagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRIPZ shNS

    Plasmid

    #127696

    Purpose

    Doxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouse

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shNS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVS1-VIR2

    Plasmid

    #134745

    Purpose

    Virulence and replication helper

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    vir gene cluster
    

    Use

    Vir gene expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGP-CMV-NES-jRGECO1a

    Plasmid

    #61563

    Purpose

    Red fluorescent calcium sensor protein

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Insert

    NES-jRGECO1a
    

    Use

    Tags

    nuclear export signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCFJ1532 - pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]

    Plasmid

    #159807

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-Lifeact-7

    Plasmid

    #54491

    Purpose

    Localization: Action, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mCherry-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. SigmaR1-mNeonGreen

    Plasmid

    #226566

    Purpose

    Encodes SigmaR1 protein labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge