We narrowed to 36,658 results for: ELL
-
Plasmid#174819PurposeMammalian expression of SpCas9 prime editor 2 with P2A-BSD markerDepositorInsertPE2-P2A-BSD
TagsP2A-BSD and SV40 bpNLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A
Plasmid#154773Purposeplasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertG1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
TagsFLAGExpressionMammalianMutationY125A in G1PylS, hybrid PylT with A41AA and C55A …PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_sgRNA_hygro-tagBFP-lox5171
Plasmid#162070Purpose3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistanceDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralExpressionMammalianPromoterU6, EF1aAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH_SBP-mCherry-GPI
Plasmid#65299Purposesynchronize trafficking of mCherry-GPI from the ER (RUSH system)DepositorInsertGPI anchor
UseLentiviralTagsIL-2 Signal Sequence and Streptavidin Binding Pro…PromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVTU_260_BirA
Plasmid#63682PurposeEncodes E. coli biotin ligase gene (BirA) for expression in yeast cellsDepositorInsertBirA
Tags6X Histidine and TEV cleavage siteExpressionYeastPromoterADH1/yeastAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-EF1A>Tet3G:IRES:Neo
Plasmid#198756PurposeVector encodes a Tet-On Advanced transactivator under control of a constitutive EF1α promoter and confers resistance to the antibiotic G418DepositorInsertTet3G, IRES, Neo
UseLentiviralPromoterEF1AAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HIFR
Plasmid#192946PurposeLentiviral production of HIF-1alpha reporterDepositorInsertHIF-1alpha reporter
UseLentiviralPromotermCMVAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Neo-TRE-CMV-Cre-rtTA
Plasmid#165457PurposeTetracycline or doxycycline inducible CRE expression from AAVS1 locusDepositorInsertCRE
ExpressionMammalianPromoterTight TRE promoterAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
HyperADAR control
Plasmid#166969PurposeExpress the Hyperactive catalytic domain of Drosophila ADAR and linker at the 5' end of this domain, in the MSCV-IRES-GFPDepositorInsertlinker-hyperADAR
UseRetroviralTagslinker-HyperADARExpressionMammalianPromoterMSCVAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
ORF3a-EGFP
Plasmid#165121Purposemammalian expression and localizationDepositorAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2-CAG::Palmbow
Plasmid#158993PurposeMulticolor membrane-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing palmitoylated mCherry, mEYFP or mCerulean upon Cre recombination)DepositorInsertPalmbow
TagsThe default expressed EBFP2 is fused to H2B; oth…ExpressionMammalianPromoterCAGAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Lyn11-FRB
Plasmid#155228Purposemembrane anchor for FKBP-FRB systemDepositorInsertMembrane targeted (through Lyn11) FRB for use with FKBP-FRB dimerization system
ExpressionMammalianAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-mVenus-Twin-Strep
Plasmid#113891PurposeMammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusionDepositorTypeEmpty backboneUseLentiviralTags3C-mVenus-Twin-StrepPromoterCMV-MIE-TetO2Available SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-20X-UAS-ADARcd
Plasmid#81173Purposeidentification of cell-specific targets of RNA binding proteinsDepositorInsertUAS-ADARcd
ExpressionInsectPromoterUASAvailable SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-CMV-CC3AI
Plasmid#78909PurposeIndicator of caspase-3-like protease activityDepositorInsertCC3AI
UseLentiviralTagsHA and MycMutationS320A, deletion of AA1-200PromoterCMVAvailable SinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Tet2 promoter F
Plasmid#63879PurposeLuciferase reporter plasmid used to study transcriptional control of murine Tet2DepositorInsertTet2 Promoter fragment F
UseLuciferaseExpressionMammalianAvailable SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-PTEN 3'UTR
Plasmid#28104DepositorInsertPTEN 3'UTR (Pten Mouse)
ExpressionMammalianAvailable SinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHA-PUMA DeltaBH3
Plasmid#16589DepositorAvailable SinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.CD19-FMC63.218.CAR-2G
Plasmid#194457PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-CD19 CAR
UseLentiviralAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry-sesRNA-smFlag-tTA2-W3SL
Plasmid#192066PurposeExpression of mCherry, sesRNA in mammalian cells, with smFlag and tTA2 as efRNA.DepositorInsertsesRNA for Mouse Ctip2
UseAAVExpressionMammalianPromoterhSynAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only