We narrowed to 62,156 results for: cloning

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p16 nlsGPS1

Plasmid

#101081

Purpose

GA biosensor expression plasmid

Depositor

Wolf Frommer, Alexander Jones

Article

Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

Insert

nlsGPS1

Tags

cMyc

Expression

Plant

Promoter

p16 (AT3G60245)

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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HNF1B_pLX307

Plasmid

#98342

Purpose

Lentiviral expression of HNF1B

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

HNF1B (HNF1B Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDicAID_nCas9-PmCDA-2A-NptII_ETR

Plasmid

#91695

Purpose

Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

Depositor

Akihiko Kondo

Article

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

PcUbi

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pTetON-DeAct-GS1

Plasmid

#89454

Purpose

Tet/Dox-inducible expression of EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-GS1 (GSN Human)

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning cytoplasmic Gsn amino acids 5…

Promoter

TRE3G

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH1_WT

Plasmid

#81921

Purpose

Gateway Donor vector containing IDH1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH1 (IDH1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_LIG1_WT_V5

Plasmid

#83006

Purpose

Gateway Donor vector containing LIG1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

LIG1 (LIG1 Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CFLAR_WT_V5

Plasmid

#82936

Purpose

Gateway Donor vector containing CFLAR, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

CFLAR (CFLAR Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGCS-N3(3xFLAG)

Plasmid

#85720

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

3xFLAG

Available Since

Feb. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pGCS-N2(3xHA)

Plasmid

#85719

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

3xHA

Available Since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-PRM-B0034-geneVI

Plasmid

#80858

Purpose

Accessory plasmid (encodes M13 gene VI)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Insert

M13 gene VI

Expression

Bacterial

Promoter

Lambda Prm

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-2xHA-DsRed

Plasmid

#80822

Purpose

Vector containing a cassette for scarless insertion of 2xHA and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Kate O'Connor-Giles

Insert

2xHA-piggyBac-DsRed

Use

Unspecified

Tags

2xHA

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAB061i

Plasmid

#79226

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-HA4; PlacIq lacI

Expression

Bacterial

Promoter

Ppro1; PlacIq

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pRS415_pGal-nCas9(D10A)

Plasmid

#79618

Purpose

Expresses nCas9(D10A) in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Expression

Yeast

Mutation

D10A for nickase Cas9

Promoter

pGal1

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pET-6His-MBP-MpAgo

Plasmid

#79249

Purpose

This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression

Depositor

Jennifer Doudna

Article

Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p

Insert

Marinitoga piezophila Argonaute

Use

CRISPR

Tags

6XHis-tag + MBP + PreScission Cleavage Site

Expression

Bacterial

Promoter

T7

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pJMK077: CaMKIIa QuasAr2-TS-GCaMP6f

Plasmid

#72304

Purpose

Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

Depositor

Adam Cohen

Article

Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

Insert

CaViar

Use

Lentiviral

Tags

TS

Expression

Mammalian

Promoter

CaMKIIa

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 IL6

Plasmid

#67061

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IL6 (IL6 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
Prab-3::NLS::GFP

Plasmid

#68120

Purpose

Nuclear Localized GFP for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

GFP

Expression

Worm

Promoter

rab-3

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-FLIM-AKAR

Plasmid

#63058

Purpose

Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKAR

Use

AAV

Expression

Mammalian

Promoter

EF-1α

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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TGFbetaR1 (T204D)-pcw107-V5

Plasmid

#64629

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

TGFBR1 (transcript variant 1) (TGFBR1 Human)

Use

Lentiviral

Tags

V5

Mutation

T204D

Promoter

PGK

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-flag-mZscan4d-polyA

Plasmid

#61831

Purpose

For mRNA synthesis or expression in mammalian cells

Depositor

Yi Zhang

Article

Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

Insert

Zscan4d (Zscan4d Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV and T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only

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