59,541 results

1. pMK485 (3mAID-Hygro)

   Plasmid

   #214397

   Purpose

   3mAID for N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   3mAID-Hygro
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. phROSA26-Tet-HA-JSAP1_WT

   Plasmid

   #208772

   Purpose

   Tetracycline inducible expression vector for HA-JSAP1_WT, used as a donor plasmid for HA-JSAP1_WT knock-in at the human ROSA26 locus

   Depositor

   Katsuji Yoshioka

   Article

   Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

   Insert

   JSAP1 (MAPK8IP3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLIB-WDR61

   Plasmid

   #231729

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Insert

   WDR61 (SKIC8 Human)
   

   Expression

   Insect

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET151-GST-PP-RPN13 (19-132)

   Plasmid

   #73742

   Purpose

   Expression of human RPN13 PRU domain in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN13 (ADRM1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   PRU domain only (residues 19-132)

   Promoter

   T7

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pRSFDuet-sumo-h-cGAS

   Plasmid

   #127161

   Purpose

   expression of human cGAS protein

   Depositor

   Thomas Tuschl

   Article

   Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

   Insert

   MB21D1 (CGAS Human)
   

   Tags

   His-SUMO tag

   Expression

   Bacterial

   Mutation

   None

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_H2AFX_WT

   Plasmid

   #81937

   Purpose

   Gateway Donor vector containing H2AFX, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   H2AFX (H2AX Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. LRP8-bio-His

   Plasmid

   #51879

   Purpose

   Expresses full-length Low-density lipoprotein receptor-related protein 8 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   LRP8 (LRP8 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. dCas9-CBP FL

   Plasmid

   #179550

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-N1 human cofilin WT

   Plasmid

   #50859

   Purpose

   expresses human cofilin fused to EGFP in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 (CFL1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. TagBFP2-PIP4K2A

    Plasmid

    #202726

    Purpose

    expresses fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    mTagBFP2:PIP4K2A (PIP4K2A Human)
    

    Expression

    Mammalian

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-MS2-HA-HsStaufen2-T1_V

    Plasmid

    #147741

    Purpose

    Mammalian Expression of HsStaufen2-T1

    Depositor

    Elisa Izaurralde

    Insert

    HsStaufen2-T1 (STAU2 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpCas9-NG-SsAPOBEC3B

    Plasmid

    #198536

    Purpose

    Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-SsAPOBEC3B
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO-RFP-shCntrl

    Plasmid

    #69040

    Purpose

    Lentiviral expression of control shRNA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    control shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. EF1a-EGFP-SKL

    Plasmid

    #170166

    Purpose

    Mammalian Expression of Ef1a-EGFP-SKL

    Depositor

    Christopher Moxham

    Insert

    EGFP-SKL
    

    Expression

    Mammalian

    Mutation

    SKL

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAX APLP1

    Plasmid

    #30141

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Like Protein 1 (APLP1 Human)
    

    Expression

    Mammalian

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

16. HA-GR

    Plasmid

    #215094

    Purpose

    Expresses HA-tagged Glucocorticoid Receptor in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    NR3C1 (NR3C1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-WT-STAT1

    Plasmid

    #71454

    Purpose

    Lentiviral expression of WT-STAT1

    Depositor

    George Stark

    Article

    Cheon et al Proc Natl Acad Sci U S A. 2009 Jun 9;106

    Insert

    STAT1 (STAT1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Promoter

    CMV

    Available Since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. LV-YFP

    Plasmid

    #26000

    Depositor

    Elaine Fuchs

    Article

    Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

    Insert

    H2B-YFP (H2BC21 Human, A. victoria)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. 4X PRE TK luc

    Plasmid

    #206159

    Purpose

    Luciferase reporter construct containing 4 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    4X PRE TK luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA Nprl3 pRK5

    Plasmid

    #46330

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Nprl3 (NPRL3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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