We narrowed to 12,771 results for: NUC
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Plasmid#164644Purposetag TRF1 with GFP and HaloDepositorAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only
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pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1
Plasmid#122084PurposeExpress M.EcoGII fused to TRF1 with detabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII-V5-TERF1
UseRetroviralTagsV5 tag on Nter of TERF1ExpressionBacterial and MammalianAvailable SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2+8 Flag2AUF1(p45)
Plasmid#135947PurposeExpresses AUF1p45 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.DepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
TagsFlag2ExpressionMammalianPromoterCMV and SP6Available SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-GST-eIF4E K119A
Plasmid#112818PurposeExpresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cellsDepositorInsertEif4e (Eif4e Mouse)
TagsGSTExpressionBacterialMutationK119 mutated to A, increasing affinity for cap st…PromoterT7Available SinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-NQO1
Plasmid#177140PurposeBacterial vector for expression of an N-terminal GST fusion of NQO1 with a TEV protease site located between the GST tag and NQO1DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE-Flag-Cdk9-T186A-IRES-eGFP
Plasmid#28097DepositorInsertCdk9 (CDK9 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationContains T186A mutation in Cdk9Available SinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
VN-Sph1
Plasmid#89755Purposeexpresses human Synphilin fused to the N-terminal part of VenusDepositorAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 proHB-EGF NC
Plasmid#11605DepositorInsertHB-EGF (HBEGF Human)
ExpressionMammalianMutationNon-cleavable HB-EGF: Pro149 to Thr160 replaced w…Available SinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
5HT2C-RNA2-Flag
Plasmid#79679Purposeserotonin receptor 2C isoform 2 with C-terminal Flag tag, non-edited isoformDepositorAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
EGFP-BAF_L58R
Plasmid#101776PurposeExpresses EGFP tagged mutant BAF (L58R) in human cellsDepositorInsertBAF (BANF1) (BANF1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationL58R mutationPromoterEF1aAvailable SinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
ESR1-luciferase
Plasmid#113351Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 geneDepositorInsertESR1-enhancer (ESR1 Human)
UseLuciferaseAvailable SinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_RRMs
Plasmid#157864Purposeexpress His tagged hnRNPA2 RRMsDepositorAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-dGFP-JARID1C
Plasmid#74776Purposeretroviral expression of JARID1CDepositorAvailable SinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-KPNA2 zeo
Plasmid#192330PurposeLentiviral expression vector for an inducible 3xFLAG-KPNA2DepositorAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICSL11056
Plasmid#68258PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plantsDepositorInsertPromoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s
UseSynthetic BiologyExpressionPlantAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBactin-Tubg1-mEGFP
Plasmid#196870PurposeExpression of the gamma-tubulin fused to enhanced (E)-GFPDepositorInsertTubg1-mEGFP (Tubg1 Mouse)
ExpressionMammalianPromoterChicken bactin (plus Chicken bactin intron)Available SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
BLADE-182
Plasmid#134914PurposesgRNA targeting GFP to be used in nanoblade systemDepositorInsertGFP
UseCRISPR and Synthetic BiologyPromoterU6Available SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSP2135
Plasmid#72248PurposeHuman expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926A, and D1135EPromoterCMVAvailable SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
3XFlag pCMV5-TOPO TAZ (S89A)
Plasmid#24815DepositorAvailable SinceMay 14, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
5HT2C-RNA1-FLAG
Plasmid#79678Purposeserotonin receptor 2C RNA isoform 1 with C-terminal flag tagDepositorAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNS20-SpCas9-SNAP
Plasmid#113717PurposeBacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-StefinA
Plasmid#182419PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-stefinA fusionDepositorInsertVNp-mNeongreen-stefin (CSTA Human)
TagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
CoV2-N-B.1.1.7
Plasmid#177958PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.DepositorInsertSARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
ExpressionMammalianMutationD3L,R203K,G204R,S235FAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA-Cre-GpNLuc
Plasmid#208382PurposeDerived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2.DepositorTypeEmpty backboneUseLentiviral and LuciferaseExpressionMammalianMutationWTAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-Bsa4_ZV-NS5-RdRp
Plasmid#179847PurposeThe RdRp domain (with a 6-histidine tag) from Zika virus nonstructural protein 5 (French Polynesia strain, GenBank accession number ARU07075.1) encompassing residues 270 to 89DepositorInsertZika Virus Non-structural protein 5 RdRp domain
Tags6-histineExpressionBacterialAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
POLR2A-N CRISPR pX330
Plasmid#124495PurposeA CRISPR plasmid for targeting the N-terminus coding region of human POLR2ADepositorInsertPOLR2A targeting CRISPR
UseCRISPRPromoterU6 promoterAvailable SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV.5’VIM-eGFP-KI
Plasmid#170547PurposeExpresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP flanked by homology arms for VIMDepositorInsertsLeft Homology arm for VIM knockin
Right Homology arm for VIM knockin
UseCRISPR and LentiviralAvailable SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-INTS3-FL
Plasmid#128415PurposeExpress INTS3 full-length in E. coli with a GST tag (N-terminal)DepositorAvailable SinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJET-CMV-GFP-P2A-K20-P2A-mCherry
Plasmid#185618PurposeRibosomal stalling reporter: positive controlDepositorInsertGFP-K20(AAA)-mCherry
ExpressionMammalianPromoterCMVAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-CMV-GFP-P2A-K0-P2A-mCherry
Plasmid#185619PurposeRibosomal stalling reporter: emptyDepositorInsertGFP-empty-mCherry
ExpressionMammalianPromoterCMVAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only