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CMVp-dCas9-3xNLS-VP64 (Construct 1)

Plasmid

#55195

Purpose

Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.

Depositor

Timothy Lu

Publication

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9

Use

CRISPR and Synthetic Biology

Tags

VP64

Expression

Mammalian

Promoter

CMV/hUBC

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAG425GPD-EGFP-ccdB

Plasmid

#14322

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Yeast

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCDNA5/FRT/TO_HaloTag7_T2A_EGFP

Plasmid

#169325

Purpose

Expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Publication

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Type

Empty backbone

Expression

Mammalian

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTHSSe_50

Plasmid

#109248

Purpose

incW BbsI cloning vector

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pBBR8k-GFPuv

Plasmid

#106385

Purpose

L-arabinose-inducible araBAD promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Publication

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

araBAD

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pICH47772

Plasmid

#48004

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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pHAGE-BCL2

Plasmid

#116717

Purpose

Lentiviral expression of BCL2

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BCL2 (BCL2 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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TOPO Cre-2A-GFP

Plasmid

#68450

Purpose

TOPO construct expressing Cre-2A-GFP for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Cre-2A-GFP

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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MXS_E2-Crimson

Plasmid

#62410

Purpose

MXS_chaining vector with E2-Crimson

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

E2-Crimson

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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LentiV_Puro

Plasmid

#111886

Purpose

Lentiviral expression of cDNA with puromycin resistance gene

Depositor

Christopher Vakoc

Publication

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGL3-U6-sgRNA-BFP

Plasmid

#107722

Purpose

For expression of sgRNA from the U6 promoter with BFP expression simultaneously

Depositor

Xingxu Huang

Publication

Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

Type

Empty backbone

Use

CRISPR

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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ZIKV_Sensor_27B_LacZ

Plasmid

#75006

Purpose

Toehold switch sensor to detect Zika Virus

Depositor

James Collins, Alexander Green

Publication

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_sensor_27B_LacZ

Tags

LacZ

Expression

Bacterial

Promoter

T7

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHAGE DN Cullin5

Plasmid

#41916

Depositor

Stephen Elledge

Publication

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

CUL5 (CUL5 Human)

Use

Lentiviral

Available since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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shLacZ

Plasmid

#42559

Depositor

William Hahn

Publication

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shLacZ

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pSimpleII-NmCas9-FLAG

Plasmid

#47872

Purpose

FLAG tagged NmCas9 with no NLS.

Depositor

Erik Sontheimer, James Thomson

Publication

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NmCas9

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-BRSK2

Plasmid

#23432

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BRSK2 (BRSK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pTol2-10xUAS-Voltron

Plasmid

#119039

Purpose

UAS-driven expresion of Voltron in zebrafish

Depositor

Eric Schreiter

Publication

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Use

Zebrafish expression

Promoter

E1b

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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mCarmine in pRSETB

Plasmid

#109484

Purpose

bacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)

Depositor

Oliver Griesbeck

Publication

Fabritius et al Cell Chem Biol. 2018 Dec 20;25(12):1554-

Insert

mCarmine

Tags

N-terminal histidine tag in vector

Expression

Bacterial

Promoter

T7

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB435

Plasmid

#74825

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-AtU3b/LacZ

Plasmid

#66199

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Publication

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

AtU3b

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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