We narrowed to 5,415 results for: PEP;

1. pAW-BiP-Nb127D01-mCherry-KDEL

   Plasmid

   #171575

   Purpose

   Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane

   Depositor

   Hidde Ploegh, Norbert Perrimon

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   BiP-Nb127D01-mCherry-KDEL (CXCR2 Human)
   

   Use

   Tags

   BiP signal peptide and mCherry-KDEL

   Expression

   Insect

   Mutation

   Promoter

   fly actin5C promoter

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAW-BiP-NbVHH05-mCherry-KDEL

   Plasmid

   #171574

   Purpose

   Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane

   Depositor

   Hidde Ploegh, Norbert Perrimon

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   BiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)
   

   Use

   Tags

   BiP signal peptide and mCherry-KDEL

   Expression

   Insect

   Mutation

   Promoter

   fly actin5C promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. R619-M96-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP

   Plasmid

   #166020

   Purpose

   mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD) optimized for serology

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S )
   

   Use

   Tags

   3C-His8-SBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA-GFP-EBA175-Flag(E1418K/A1419R/S1421R/P1424Q/Y1426S)

   Plasmid

   #155010

   Purpose

   Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Use

   Tags

   Flag, GFP, and signal peptide (residues 1 to 32) …

   Expression

   Mammalian

   Mutation

   E1418K/A1419R/S1421/P1424Q/Y1426S; insert codes f…

   Promoter

   CMV

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HA tagged mid ELL2 M133, 138, 186ILeu

   Plasmid

   #127276

   Purpose

   Expresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains middle HA tag that doesn't disrupt function

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (ELL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M133, 138, 186 to Ileu, HA tag at XbaI after Met …

   Promoter

   pEF1a

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCEP4-BG505.SOSIP-gp160-QES.i05.c06-754*

   Plasmid

   #123275

   Purpose

   Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (BG505) Env
   

   Use

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; SOSIP mutations; …

   Promoter

   CMV

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Rescue myc-PCDHGC5 mRNA

   Plasmid

   #122229

   Purpose

   Rescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA (plasmid 122227). With five silent mutations at sh1 shRNA target site.

   Depositor

   Angel de Blas

   Article

   Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

   Insert

   PCDHGC5 (Pcdhgc5 Rat)
   

   Use

   Tags

   9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…

   Expression

   Mammalian

   Mutation

   Silent mutations are in five consecutive codons …

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - C130S, E446C

   Plasmid

   #60394

   Purpose

   Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for crosslinking polyglutamate peptide

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB1 (TUB1 Budding Yeast, Synthetic)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   internal 6xHis (see supplement figure 4), amino a…

   Promoter

   GAL

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-endo-GsCT

   Plasmid

   #205516

   Purpose

   Endosome-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   endo-GsCT (GNAS Human, Synthetic)
   

   Use

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXS-mitoGshF-blasticidin

    Plasmid

    #210314

    Purpose

    Expresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cells

    Depositor

    Kivanc Birsoy

    Article

    Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

    Insert

    Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
    

    Use

    Retroviral

    Tags

    FLAG peptide, Homo sapiens ACO2 mitochondrial tar…

    Expression

    Mutation

    Codon optimized for expression in human cells

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-hSyn-miniDq-mCherry-P2A-HA-KORD

    Plasmid

    #204359

    Purpose

    AAV vector for coexpression of miniDq and KORD under the control of human synapsin promoter

    Depositor

    Masao Doi

    Article

    Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

    Insert

    miniDq-mCherry-P2A-HA-KORD
    

    Use

    AAV

    Tags

    HA (for KORD) and mCherry (for miniDq)

    Expression

    Mutation

    For miniDq, the third intracellular loop (ICL3) o…

    Promoter

    hSyn

    Available since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGS_101

    Plasmid

    #160541

    Purpose

    Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

    Depositor

    Susan Lindquist, Li-Huei Tsai

    Article

    Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

    Insert

    Apolipoprotein E e4 (APOE Human)
    

    Use

    Tags

    Kar2 signal sequence

    Expression

    Yeast

    Mutation

    e4 allele

    Promoter

    GAL1

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-EIN-FTO(32-505)

    Plasmid

    #186466

    Purpose

    Expresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein)

    Depositor

    Vincenzo Venditti

    Article

    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

    Insert

    Fat mass obesity associated protein (FTO Human)
    

    Use

    Tags

    6 Histidine-EIN (N-terminal domain of E.coli Enzy…

    Expression

    Bacterial

    Mutation

    amino acid 32-505

    Promoter

    T7

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMSCVpuro-Mito-Pericam

    Plasmid

    #87381

    Purpose

    Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.

    Depositor

    Björn Stork

    Article

    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do

    Insert

    Mito-Pericam [ratiometric]
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Retroviral 5' LTR promoter

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-26b-Nb127D01-ALFA-His

    Plasmid

    #171568

    Purpose

    Bacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    Nb127D01-ALFA-His (CXCR2 Human)
    

    Use

    Tags

    ALFA-tag/His-tag and PelB signal peptide

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGS_128

    Plasmid

    #160651

    Purpose

    Expresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

    Depositor

    Susan Lindquist, Li-Huei Tsai

    Article

    Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

    Insert

    Apolipoprotein E e3 (APOE Human)
    

    Use

    Tags

    Kar2 signal sequence

    Expression

    Yeast

    Mutation

    e3 allele

    Promoter

    pZ estradiol inducible promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hSyn-HA-PGRN-LAMP1TM-IRES-GFP

    Plasmid

    #234877

    Purpose

    Lentiviral plasmid expressing HA-tagged human progranulin that is fused to the transmembrane domain and cytosolic tail of LAMP-1. Enables lysosomal delivery of progranulin without secretion.

    Depositor

    Andrew Arrant

    Article

    Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

    Insert

    Progranulin (GRN Human)
    

    Use

    Lentiviral

    Tags

    HA tag and LAMP-1 transmembrane domain and cytoso…

    Expression

    Mammalian

    Mutation

    Promoter

    hSYn

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDHa_mDDX5_FL_wt

    Plasmid

    #88869

    Purpose

    constitutive expression of Ha-DDX5 in mammalian cells

    Depositor

    Dan Littman

    Article

    Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

    Insert

    DDX5 (Ddx5 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CRHR1-DuET

    Plasmid

    #213215

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CRHR1 (CRHR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Ad:2CMV_A2R2-N25K3

    Plasmid

    #122242

    Purpose

    Expresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

    Depositor

    Corey Neu

    Article

    Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

    Inserts

    C-terminal end of Nesprin-3 (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    

    Use

    Adenoviral

    Tags

    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge