We narrowed to 56,765 results for: plasmid
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Plasmid#39347DepositorInsertscFv-Fc-fusion specific for Notch2 (clone B6) (NOTCH2 Human)
Tags3xFLAG, 6xHis, and human FcExpressionMammalianAvailable SinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGrin1
Plasmid#124852PurposeMutagenesis of Grin1DepositorInsertGrin1 (Grin1 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2
Plasmid#166014Purposemammalian expression of HCoV-HKU1 soluble spike trimer proteinDepositorInsertHCoV-HKU1 S (spike)
Tags3C-His8-Strep2x2ExpressionMammalianPromoterCMV51Available SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-EGFP-P2A-EGFPf-WPRE-HGHpA
Plasmid#74513PurposeAAV vector that use human synapsin-1 promoter to drive the expression of EGFP and membrane-targeted EGFPf linked by self-cleaving P2A peptide.DepositorInsertEGFP-p2A-EGFP-f
UseAAVAvailable SinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET29-bdSUMO-Anti-ALFA nanobody
Plasmid#212311PurposeExpression of anti-ALFA nanobodyDepositorInsertanti-ALFA nanobody
TagsbdSUMOExpressionBacterialPromoterT7Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJSX-GL3
Plasmid#11980PurposeReporter gene with mouse c-jun promoter and point mutation of MEF2 binding siteDepositorAvailable SinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRVL-2
Plasmid#104580PurposeContains mouse IgG2c CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functionsDepositorInsertMouse IgG2c CH1/CH2/CH3
ExpressionMammalianPromoterCMVAvailable SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmIL-6 mut NF-kB
Plasmid#61293Purposedrives luciferase from mouse IL-6 promoter with mutation in NF-kB siteDepositorInsertIL-6 promoter (Il6 Mouse)
UseLuciferaseExpressionMammalianMutationMutated NF-kB binding site from GTGGGATTTTCCCA to…PromoterIL-6 promoter with mutant NF-kB binding siteAvailable SinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-GCaMP6m-XC
Plasmid#118975PurposeImproved genetic encoded calcium sensor based on GCaMP6mDepositorInsertGCaMP6m-XC
UseAAVAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-eYFP
Plasmid#104056PurposeAn AAV genome with tet-inducible expression of the fluorescent protein eYFPDepositorInsertEYFP
UseAAVPromoterTREAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-102.1F10-IgG2/λ
Plasmid#50367PurposeExpresses grass pollen allergen Phl p 7 specific human IgG2/λ antibody isotype (102.1F10-IgG2/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human Gamma 2 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
ExpressionMammalianPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
PGL3-NOXA-N1
Plasmid#26112DepositorAvailable SinceSept. 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-mCherry
Plasmid#109421PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiHer24D5-5_synNotch_Gal4VP64
Plasmid#85423PurposeLentiviral vector for expression of anti-Her2 scFv 24D5-5 Gal4VP64 synthetic NotchDepositorInsertanti-Her2_4D5-5 synNotch
UseLentiviralPromoterPGKAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
OCT4-GFP-2A-PURO transgenic reporter
Plasmid#52379PurposeBAC recombineering generated construct, used as a reporter for OCT4 expressionDepositorInsertsGFP-2A-puro
OCT4
ExpressionMammalianPromoterOCT4Available SinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-nEF-dCas9
Plasmid#106430PurposeExpresses SpdCas9 from the nEF promoter in AAV backboneDepositorInsertnEF-dCas9
UseAAV and CRISPRTagsHA-NLS and NLSExpressionMammalianPromoternEFAvailable SinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
v1em-Nterm-PE2max-U6-sgRNA
Plasmid#198732PurposeAAV genome encoding N-terminal PE2max and U6 expression cassetteDepositorInsertNterm PE2max-NpuN
UseAAVPromoterEFSAvailable SinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_ORI_CMV
Plasmid#99312PurposeLuciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertCMV enhancer
UseLuciferase; Starr-seq luciferase validation vectorExpressionMammalianAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-KP1
Plasmid#206504PurposeAAV packaging vector containing AAV2 rep and KP1 cap and partial AAV2 p5 promoterDepositorInsertKP1 capsid
UseAAVPromoterp5Available SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple (bidirectional) GasS
Plasmid#196055PurposeEncodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorInsertsUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only