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We narrowed to 1,269 results for: MS2

Showing: 751 - 780 of 1269 results

  1. pYPQ-CBE-Cas9n-Act3.0

    Plasmid
    #178955
    Purpose
    It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178955-plasmid-map-sequence-id-458203

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  2. pYPQ-ABE-Cas9n-Act3.0

    Plasmid
    #178956
    Purpose
    It consists of a Cas9 nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    ABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178956-plasmid-map-sequence-id-354328

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  3. pCK341

    Plasmid
    #192640
    Purpose
    Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102
    Inserts
    Sp.pCas9-dSpRY
    BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR and Synthetic Biology
    Mutation
    SoxS has R93A and S101A mutations and dSpRY has s…
    Promoter
    BBa_J23107 and Sp.pCas9
    Available Since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    192640-plasmid-map-sequence-id-381518

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  4. pJZC34

    Plasmid
    #62331
    Purpose
    sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells
    Depositor
    Stanley Qi, Wendell Lim
    Article
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Inserts
    sgRNA + 2x MS2(wt+f6) binding module
    MCP-VP64
    Use
    Lentiviral
    Tags
    VP64
    Expression
    Mammalian
    Mutation
    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA
    Promoter
    CMV and U6
    Available Since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    62331-plasmid-map-sequence-id-102253

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  5. pYPQ-ABE-SpRYn-Act3.0

    Plasmid
    #178959
    Purpose
    It consists of a SpRY nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    ABE8e-SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178959-plasmid-map-sequence-id-354322

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  6. pCK340

    Plasmid
    #192639
    Purpose
    Expresses dSpG and MCP-SoxS(R93A/S101A) on p15A-CmR
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102
    Inserts
    Sp.pCas9-dSpG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR and Synthetic Biology
    Mutation
    SoxS has R93A and S101A mutations and dSpG has se…
    Promoter
    BBa_J23107 and Sp.pCas9
    Available Since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    192639-plasmid-map-sequence-id-381517

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  8. pCK668

    Plasmid
    #192637
    Purpose
    Expresses dCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102
    Inserts
    Sp.pCas9-dCas9-NG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR and Synthetic Biology
    Mutation
    SoxS has R93A and S101A mutations and dCas9-NG ha…
    Promoter
    BBa_J23107 and Sp.pCas9
    Available Since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    192637-plasmid-map-sequence-id-381515

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  10. pYPQ-dzCas9-NG-Act3.0

    Plasmid
    #158415
    Purpose
    CRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dzCas9-NG-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158415-plasmid-map-sequence-id-331006

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  11. pYPQ-dAaCas12b-Act3.0

    Plasmid
    #158413
    Purpose
    CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    158413-plasmid-map-sequence-id-337077

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  12. pCK669

    Plasmid
    #192638
    Purpose
    Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102
    Inserts
    Sp.pCas9-dxCas9-NG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR and Synthetic Biology
    Mutation
    SoxS has R93A and S101A mutations and dxCas9-NG h…
    Promoter
    BBa_J23107 and Sp.pCas9
    Available Since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    192638-plasmid-map-sequence-id-381516

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  13. MCP-Rep-X-RT∆R

    Plasmid
    #239384
    Purpose
    For circular RNA-mediated inverse prime editor using MCP-Rep-X-M MLV-RT∆RNase H in HEK294T cells
    Depositor
    Caixia Gao
    Article
    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:
    Insert
    MCP, Rep-X, M-MLV-RT(∆RNase H)
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ∆RNase H in M-MLV RT
    Promoter
    CMV
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239384-plasmid-map-sequence-id-481789

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  14. pPPC002

    Plasmid
    #171139
    Purpose
    For integration of J1-J23117-sfGFP, Sp.pCas9-dCas9, and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Inserts
    J1-BBa_J23117-sfGFP
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    J1-BBa_J23117 and Sp.pCas9, BBa_J23107
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    171139-plasmid-map-sequence-id-344756

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  15. pJZC116

    Plasmid
    #62344
    Purpose
    sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, marked by BFP
    Depositor
    Stanley Qi, Wendell Lim
    Article
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Inserts
    sgRNA + 2x MS2 (wt+f6) binding module
    MCP-VP64
    Use
    Lentiviral
    Tags
    VP64
    Expression
    Mammalian
    Mutation
    Targets CXCR4 , sequence: GCAGACGCGAGGAAGGAGGGCGC
    Promoter
    CMV and U6
    Available Since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62344-plasmid-map-sequence-id-102267

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  17. pYJ15-PB-U6-Nkx6.1-acti-gRNA1

    Plasmid
    #131059
    Purpose
    Activation of NKX6.1 via SAM system
    Depositor
    Xiaojun Lian
    Insert
    NKX6.1 activation gRNA1 (NKX6-1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131059-plasmid-map-sequence-id-256775

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  18. pYJ16-PB-U6-Nkx6.1-acti-gRNA2

    Plasmid
    #131060
    Purpose
    Activation of NKX6.1 via SAM system
    Depositor
    Xiaojun Lian
    Insert
    NKX6.1 activation gRNA2 (NKX6-1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131060-plasmid-map-sequence-id-256750

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  19. pYJ17-PB-U6-Nkx6.1-acti-gRNA3

    Plasmid
    #131061
    Purpose
    Activation of Mafa via SAM system
    Depositor
    Xiaojun Lian
    Insert
    NKX6.1 activation gRNA3 (NKX6-1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131061-plasmid-map-sequence-id-256770

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  20. pYJ12-PB-U6-ND1-acti-gRNA1

    Plasmid
    #131056
    Purpose
    Activation of NeuroD1 via SAM system
    Depositor
    Xiaojun Lian
    Insert
    NeuroD1 activation gRNA1 (NEUROD1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131056-plasmid-map-sequence-id-256748

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  21. pYJ13-PB-U6-ND1-acti-gRNA2

    Plasmid
    #131057
    Purpose
    Activation of NeuroD1 via SAM system
    Depositor
    Xiaojun Lian
    Insert
    NeuroD1 activation gRNA2 (NEUROD1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131057-plasmid-map-sequence-id-256893

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  22. pYJ14-PB-U6-ND1-acti-gRNA3

    Plasmid
    #131058
    Purpose
    Activation of NeuroD1 via SAM system
    Depositor
    Xiaojun Lian
    Insert
    NeuroD1 activation gRNA3 (NEUROD1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131058-plasmid-map-sequence-id-256735

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  23. pCSDest-ENTR-MCP-ADAR2-DD(E488Q/T490A)-V5

    Plasmid
    #192714
    Purpose
    Mammalian expression vector containing MS2 coat protein fused to ADAR2 catalytic domain (E488Q/T490A) with V5 tag
    Depositor
    Summer Thyme
    Insert
    MCP-ADAR2-DD(E488Q/T490A)-V5
    Tags
    V5
    Expression
    Mammalian
    Mutation
    E488Q/T490A
    Promoter
    CMV
    Available Since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192714-plasmid-map-sequence-id-377312

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  25. pTwist-ENTR-MCP-ADAR2-DD(E488Q/T490A)-V5

    Plasmid
    #192713
    Purpose
    Gateway entry vector containing MS2 coat protein fused to ADAR2 catalytic domain (E488Q/T490A) with V5 tag
    Depositor
    Summer Thyme
    Insert
    MCP-ADAR2-DD(E488Q/T490A)-V5
    Use
    Gateway entry vector
    Tags
    V5
    Mutation
    E488Q/T490A
    Promoter
    None
    Available Since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192713-plasmid-map-sequence-id-377311

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  26. pYJ18-PB-U6-Mafa-acti-gRNA1

    Plasmid
    #131062
    Purpose
    Activation of NKX6.1 via SAM system
    Depositor
    Xiaojun Lian
    Insert
    MAFA activation gRNA1 (MAFA Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131062-plasmid-map-sequence-id-256743

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  27. pYJ19-PB-U6-Mafa-acti-gRNA2

    Plasmid
    #131063
    Purpose
    Activation of NKX6.1 via SAM system
    Depositor
    Xiaojun Lian
    Insert
    MAFA activation gRNA2 (MAFA Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131063-plasmid-map-sequence-id-256773

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  28. pYJ20-PB-U6-Mafa-acti-gRNA3

    Plasmid
    #131064
    Purpose
    Activation of NKX6.1 via SAM system
    Depositor
    Xiaojun Lian
    Insert
    MAFA activation gRNA3 (MAFA Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131064-plasmid-map-sequence-id-256740

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Showing: 751 - 780 of 1269 results