Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,599 results for: ICL

Clear keywords
Showing: 1001 - 1040 of 1599 results

  1. MZB160

    Plasmid
    #217841
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (L8K+Q71L) for pulldown assays
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    Arf1 (ARF1 Human)
    Tags
    10x His, HaloTag, and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    L8K, Q71L
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217841-plasmid-map-sequence-id-432998

    Enlarge

  3. MZB224

    Plasmid
    #226998
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (T31N) for pulldown assays
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    Arf1 (ARF1 Human)
    Tags
    10x His, HaloTag, and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    T31N
    Promoter
    T7
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    226998-plasmid-map-sequence-id-455977

    Enlarge

  5. MZB222

    Plasmid
    #221772
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (687-885) of Big1 (ArfGEF1) for rapid nucleotide exchange and activation of Arf GTPases
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    ArfGEF1 (ARFGEF1 Human)
    Tags
    10xHis-SUMO
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    221772-plasmid-map-sequence-id-455976

    Enlarge

  15. pFsynW SYT1-PGM reporter

    Plasmid
    #197278
    Purpose
    Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.
    Depositor
    Edwin Chapman
    Article
    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755
    Insert
    SYT1 and HaloTag (Syt1 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Mutation
    T15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82A
    Promoter
    hSyn (human synapsin I promoter)
    Available Since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197278-plasmid-map-sequence-id-389096

    Enlarge

  18. pJL1-sfGFP-MOORmut

    Plasmid
    #198213
    Purpose
    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing a non-permissible minimum optimal O-linked recognition site (MOORmut) followed by a 6x-His tag
    Depositor
    Michael Jewett
    Article
    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:
    Insert
    sfGFP_MOORmut
    Tags
    6x His Tag and mutated (non-permissible) MOORmut …
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    198213-plasmid-map-sequence-id-393674

    Enlarge

  24. KG#797

    Plasmid
    #110916
    Purpose
    Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons
    Depositor
    Kenneth Miller
    Article
    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:
    Inserts
    unc-129 promoter
    STRN-1 (Sentryn)-GFP
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    Tags
    GFP (contains 3 artificial introns)
    Expression
    Bacterial
    Available Since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110916-plasmid-map-sequence-id-212580

    Enlarge

  25. RGD-I27-CC

    Plasmid
    #85393
    Purpose
    MTFM mechanosensor for integrin. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines for immobilization and site for p-azidophenylalanine incorporation & Cy3 labeling
    Depositor
    Khalid Salaita
    Article
    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:
    Insert
    RGD motif for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
    Tags
    6x His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    85393-plasmid-map-sequence-id-172696

    Enlarge

  26. pF(UG) hSyn NS34a-msGFP-A'Q-mito

    Plasmid
    #158766
    Purpose
    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Lentiviral
    Mutation
    Flexible linkers between the protease and msGFP a…
    Availability
    Academic Institutions and Nonprofits only
    158766-plasmid-map-sequence-id-313130

    Enlarge

  27. pF(UG) hSyn NS34a-msGFP-'Q-mito

    Plasmid
    #158767
    Purpose
    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Lentiviral
    Mutation
    Flexible linkers between the protease and msGFP a…
    Availability
    Academic Institutions and Nonprofits only
    158767-plasmid-map-sequence-id-313145

    Enlarge

  36. pTFG005_Spike_Inducible_3xFlag

    Plasmid
    #191578
    Purpose
    Inducible expression of SARS-CoV-2 Spike protein with C-terminal 3x FLAG tag under TRE3G promoter and cloned into a 2nd generation lentiviral transfer plasmid (modified pLVX with Hygro resistance).
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    SARS-CoV-2 Spike Protein (S Synthetic)
    Use
    Lentiviral
    Tags
    3x FLAG
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    191578-plasmid-map-sequence-id-442483

    Enlarge

  40. CoV2-Spike-D614G

    Plasmid
    #177960
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
    Expression
    Mammalian
    Mutation
    D614G
    Available Since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177960-plasmid-map-sequence-id-348179

    Enlarge

Showing: 1001 - 1040 of 1599 results