We narrowed to 4,288 results for: PRS
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Plasmid#122070PurposeExpressing ATG1 with ATG7 3'UTRDepositorInsertserine/threonine protein kinase ATG1 (ATG1 Budding Yeast)
ExpressionYeastMutationThe native 3'UTR was replaced by ATG7 3'…Available SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA(D195A,E196A)(405)
Plasmid#122065PurposeExpressing Dhh1-PADepositorInsertDhh1-PA (DHH1 Budding Yeast)
TagsPAExpressionYeastMutationAspartic acid 195 to alanine; Glutamic acid 196 t…Available SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA(S226A,T228A)(405)
Plasmid#122066PurposeExpressing Dhh1-PADepositorInsertDhh1-PA (DHH1 Budding Yeast)
TagsPAExpressionYeastMutationSerine 226 to alanine; Threonine 228 to alanineAvailable SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKP105 (pRS416-YBR139Wp-YBR139W-PA-ADH1t)
Plasmid#106461PurposeExpresses Atg42/Ybr139w with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W
TagsPAExpressionBacterial and YeastPromoterYBR139WAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP135 (pRS406-YBR139Wp-YBR139W-PA-ADH1t)
Plasmid#106470PurposeExpresses Atg42/Ybr139w with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W
TagsPAExpressionBacterial and YeastPromoterYBR139WAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc
Plasmid#91935PurposeBacterial expression of maize SUMO1a and inactive SCE1dDepositorInserts6His-ZmSUMO1a-GG
ZmSCE1d(C94S)-Myc
Tags6His and MycExpressionBacterialMutationC94S active site mutation and Truncation of ZmSUM…Available SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSF_6His-ZmSUMO1a-GG_ZmSCE1b(C94S)-Myc
Plasmid#91933PurposeBacterial expression of maize SUMO1a and inactive SCE1bDepositorInserts6His-ZmSUMO1a-GG
ZmSCE1b(C94S)-Myc
Tags6His and MycExpressionBacterialMutationC94S active site mutation and Truncation of ZmSUM…Available SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry
Plasmid#68276PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLSDepositorInsertSte7DS NLS(4E) mCherry
TagsmCherryExpressionYeastPromoterRPS2Available SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETC-PITPbeta (splice variant 1) K61A
Plasmid#58271Purposebacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)DepositorInsertPITP beta (PITPNB Human)
TagsHISExpressionBacterialMutationK61A (abolishes PI binding)PromoterT7Available SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(Ca2+)_63/pRSETB
Plasmid#51980Purposebacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nMDepositorInsertNano-lantern_CaM_E104Q-4GS
Tags6xHisExpressionBacterialMutationS257G in Rluc8Available SinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal-RNQ1 L94A-YFP
Plasmid#18692DepositorInsertRnq1 L94A (RNQ1 Budding Yeast)
TagsYFPExpressionYeastMutationLeucine 94 mutated to Alanine (L94A)Available SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKP113 (pRS406-PRC1p-PRC1-GFP-ADH1t)
Plasmid#106464PurposeExpresses Prc1 with a C-terminal GFP tag in yeast cellsDepositorInsertPRC1
TagsGFPExpressionBacterial and YeastPromoterPRC1Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS305-LEU2-pINO4-SV40NLS-PfV-GS-Sapphire
Plasmid#203655PurposeYeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.DepositorArticleInsertPfV
TagsSapphireExpressionYeastAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS403-HIS3-pINO4-SV40NLS-PfV-GS-Sapphire
Plasmid#203658PurposeYeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm GEMs under the HIS3 promoter.DepositorArticleInsertPfV
TagsSapphireExpressionYeastAvailable SinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS305-LEU2-pINO4-SV40NLS-PfV-GS-yEmCherry
Plasmid#203656PurposeYeast integrative vector for the expression of the yEmCherry-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.DepositorArticleInsertPfV
TagsyEmCherryExpressionYeastAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKD‐SepRS-EFSep-5x tRNASep (B40 OTS)
Plasmid#52054PurposeUsed in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)DepositorInsertsSepRS
EF-Sep
5x tRNA Sep
ExpressionBacterialMutationHis67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…PromoterPtacAvailable SinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PTPRS-COMP5AP-AviTag-9xHis
Plasmid#157602PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130)
Plasmid#145843PurposeLentiviral vector genome to ectopically express human TMPRSS2 variant 1DepositorInserthuman TMPRSS2 variant 1 (TMPRSS2 Human)
UseLentiviralExpressionMammalianMutationG8VPromoterSFFVAvailable SinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)
Plasmid#86413Purposenuclear split GFP11 reporter with mCherryDepositorInsertNop1pr-GFP11-mCherry-PUS1
TagsGFP11 and mCherryExpressionYeastPromoterNOP1Available SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSJ1568 (pRS315-NOP1pr-GFP11-mCherry-SCS2TM)
Plasmid#86416PurposeONM/ER split GFP11 reporter with mCherryDepositorInsertNOP1pr-GFP11-mCherry-Scs2TM
TagsGFP11 and mCherryExpressionYeastPromoterNOP1Available SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry
Plasmid#68280PurposeSKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherryDepositorAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSJ1602 (pRS315-NOP1pr-mCherry-SCS2TM-GFP11)
Plasmid#86417Purposeluminal split GFP11 reporter with mCherryDepositorInsertNOP1pr-mCherry-SCS2TM-GFP11
TagsGFP11 and mCherryExpressionYeastPromoterNOP1Available SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSFDUET-1_VNp-anti-Lysozyme fAb-BiFC
Plasmid#214950PurposeBacterial expression of anti-chicken lysozyme Fab light and heavy chain VNp fusions. Each chain is fused to separate halves of a BiFC protein -Venus fluorescence signifies Hc/Lc heterodimer formation.DepositorInsertsVNp-anti-Lysozyme Fab heavy chain amino-BiFC fusion
VNp-anti-Lysozyme Fab light chain amino-BiFC fusion
Tags6xHis, Amino half of venus BIFC fragment, Carboxy…ExpressionBacterialPromoterT7Available SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBK-EcTrpRS(H14)-EcTyrRS(VSMA,D165G)
Plasmid#231130PurposeExpresses mutant E. coli TrpRSTyrRS from a glnS promoters for proteome-wide incorporation of tryptophan/tyrosine analogues.DepositorInsertE. coli tyrosyl tRNA synthetase
ExpressionBacterialMutationEcTrpRS: S8A, V144G, V146C; EcTyrRS: Y37V, D165G,…Available SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGZ136 (pRS406-pREB1-3FLAG-MNase-NLS)
Plasmid#72273PurposeFLAG-Mnase expressed under the REB1 promoterDepositorInsert3xFLAG tag and aa 83-231 of Mnase
PromoterREB1Available SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS305-LEU2-pHIS3-SV40NLS-PfV-GS-GFPMUT3
Plasmid#203657PurposeYeast integrative vector for the expression of the GFPMUT3-tagged nuclear localizing 40nm nucGEMs under the HIS3 promoter.DepositorArticleInsertPfV
TagsGFPMUT3ExpressionYeastAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt
Plasmid#194519PurposeDual fluorescence reporter for studying protein degradationDepositorInsertTIF4631 leader sequence
ExpressionYeastAvailable SinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PTPRS-Fc(DAPA)-AviTag-6xHis
Plasmid#157038PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertPTPRS (PTPRS Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL
Plasmid#79614PurposeExpresses dCas9-SH3 and PmCDA1-SHL in yeast cellsDepositorInsertsSpCas9
PmCDA1
TagsSH3 domain and SHLExpressionYeastMutationD10A and H840A for nuclease deficient Cas9PromoterpGal1 and pGal10Available SinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-2xOsh2 PH domain [short]
Plasmid#58829PurposeExpresses GFP-Tagged Osh2 PH domain in yeastDepositorInsert2xOsh2 PH domain [short] (OSH2 Budding Yeast)
TagsGFP and MycExpressionYeastPromoterPHO5Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal-RNQ1 153-405-YFP
Plasmid#18689DepositorInsertRnq1 153-405
TagsYFPExpressionYeastMutationPrion domain of Rnq1Available SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRS303-pGAL-SPPHO5-G-SrtAstrep-HA-HDEL (pKS82)
Plasmid#50032PurposeGalactose-inducible expression of ER-luminal S. pyogenes SrtA in yeastDepositorInsertHA-SrtA(strep)
TagsHA-HDELExpressionYeastPromoterpGALAvailable SinceJan. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKP115 (pRS406-YBR139Wp-YBR139W-GFP-ADH1t)
Plasmid#106465PurposeExpresses Atg42/Ybr139w with a C-terminal GFP tag in yeast cellsDepositorInsertYBR139W
TagsGFPExpressionBacterial and YeastPromoterYBR139WAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSP303 pRS306 pRPS2-Far1DS-NLS-mCherry
Plasmid#68279PurposeSKARS: Fus3 MAPK activity sensor: Far1DS 2xNLS mCherryDepositorInsertFar1DS NLS mCherry
TagsmCherryExpressionYeastPromoterRPS2Available SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pED73 pRS304 pRPS2-SteDS-NLS-mCitrine
Plasmid#68278PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCitrineDepositorInsertSte7DS NLS mCitrine
TagsmCitrineExpressionYeastPromoterpRPS2Available SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation
Plasmid#58830PurposeExpresses GFP-Tagged and mutated Osh2 PH domain in yeastDepositorInsert2 x Osh2 PH domain (OSH2 Budding Yeast)
TagsGFP and MycExpressionYeastMutationchanged Lysine 307 to Glutamate and Arginine 309 …PromoterPHO5Available SinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pED45: pRS306 pRPS2-Ste7DS-NLS-mCherry
Plasmid#68274PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherryDepositorInsertSte7DS NLS mCherry
TagsmCherryExpressionYeastPromoterpRPS2Available SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB6 Cterminal tail
Plasmid#60403PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoterDepositorInsertTUB2
ExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable SinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-CERT PH domain EE mutation
Plasmid#58833PurposeExpresses GFP-Tagged and mutated CERT PH domain in yeastDepositorInsertcollagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
TagsGFP and MycExpressionYeastMutationchanged Arginine 43 to GlutamatePromoterPHO5Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB2a Cterminal tail
Plasmid#60396PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) using a GAL promoterDepositorInsertTUB2
ExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
T7pro-tRNA-ITSIle intron-T7tVar1-pRS426
Plasmid#239294PurposeExpresses the tRNAIle intron bearing the 5' GGGAGA initially transcribed sequence (ITS); Plasmid #2 of the tet-on overexpression systemDepositorInsertsT7 promoter
ITS-tRNAIle intron
T7 terminator - Var1
ExpressionYeastAvailable SinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3
Plasmid#221112PurposeFAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ERDepositorInsertMFA-IgKappa-FAPoptim-STE3
ExpressionYeastPromoterSTE3Available SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-MFA-Igkappa-FAPoptim-STE3
Plasmid#221114PurposeFAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Tef1 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ERDepositorInsertMFA-IgKappa-FAPoptim-STE3
ExpressionYeastPromoterTEF1Available SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS416-CUP1p-TID-FRB-NLS-HA3
Plasmid#228009PurposeExpresses TurboID-FRB-NLS fusion from CUP1 promoter; yeast BioIDDepositorInsertTurboID-FRB
TagsHA tagExpressionYeastPromoterCUP1Available SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-CUP1p- TID-FKBP-NLS-HA3
Plasmid#228011PurposeExpresses TurboID-FKBP-NLS fusion from CUP1 promoter; yeast BioIDDepositorInsertTurboID-FKBP
TagsHA tagExpressionYeastPromoterCUP1Available SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-CHA1p-TID-FRB-NLS-HA3
Plasmid#228013PurposeExpresses TurboID-FRB-NLS fusion from CHA1 promoter; yeast BioIDDepositorInsertTurboID-FRB
TagsHA tagExpressionYeastPromoterCHA1Available SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-CHA1p- TID-FKBP-NLS-HA3
Plasmid#228015PurposeExpresses TurboID-FKBP-NLS fusion from CHA1 promoter; yeast BioIDDepositorInsertTurboID-FKBP
TagsHA tagExpressionYeastPromoterCHA1Available SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BirA-FRB-NLS-HA3
Plasmid#227985PurposeExpresses BirA-FRB-NLS fusion from ADH1 promoter; yeast BioIDDepositorInsertBirA-FRB
TagsHA tagExpressionYeastPromoterADH1Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BirA-FKBP-NLS-HA3
Plasmid#227987PurposeExpresses BirA-FKBP-NLS fusion from ADH1 promoter; yeast BioIDDepositorInsertBirA-FKBP
TagsHA tagExpressionYeastPromoterADH1Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BioID2-FRB-NLS-HA3
Plasmid#227989PurposeExpresses BioID2-FRB-NLS fusion from ADH1 promoter; yeast BioIDDepositorInsertBioID2-FRB
TagsHA tagExpressionYeastPromoterADH1Available SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only