We narrowed to 1,621 results for: nif

1. JAB3064

   Plasmid

   #190400

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proUBQ10s::GFPint-ADHt)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   proUBQ10::PIP2A-mCherry-NOSt_proUBQ10s::GFPint-ADHt
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. JAB3604

   Plasmid

   #190423

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_3XPhlFO::GFPint-ADHt)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   proUBQ10::PIP2A-mCherry-NOSt_3XPhlFO::GFPint-ADHt
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. JAB3605

   Plasmid

   #190424

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_2XPhlFO::GFPint-ADHt)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   proUBQ10::PIP2A-mCherry-NOSt_2XPhlFO::GFPint-ADHt
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. JAB3606

   Plasmid

   #190425

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_1XPhlFO::GFPint-ADHt)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   proUBQ10::PIP2A-mCherry-NOSt_1XPhlFO::GFPint-ADHt
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. JAB3236

   Plasmid

   #190401

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proTT*::GFPint_ADHt)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   proUBQ10::PIP2A-mCherry-NOSt_proTT*::GFPint_ADHt
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. JAB2938

   Plasmid

   #190395

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proTT::GFPint-NLS_ADHt)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   proUBQ10::PIP2A-mCherry-NOSt_proTT::GFPint-NLS_ADHt
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. JAB2515

   Plasmid

   #190383

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-TetRO::GFPint-NLS-ADHt)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   proUBQ10::PIP2A-mCherry-NOSt_pro35S-TetRO::GFPint-NLS-ADHt
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. JAB2511

   Plasmid

   #190380

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XRpaRO-min35S::GFPint-ADHt)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   proUBQ10::PIP2A-mCherry-NOSt_6XRpaRO-min35S::GFPint-ADHt
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. JAB2485

   Plasmid

   #190371

   Purpose

   Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-McbRO::GFPint-NLS-ADHt)

   Depositor

   Jennifer Brophy, Jose Dinneny

   Article

   Brophy et al Science. 2022 Aug 12;377(6607):747-751.

   Insert

   proUBQ10::PIP2A-mCherry-NOSt_pro35S-McbRO::GFPint-NLS-ADHt
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. JAB2463

    Plasmid

    #190370

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-QacRO::GFPint-NLS-ADHt)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_pro35S-QacRO::GFPint-NLS-ADHt
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. JAB2275

    Plasmid

    #190361

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO::GFPint-NLS-ADHt)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO::GFPint-NLS-ADHt
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. JAB2224

    Plasmid

    #190358

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::GFPint-ADHterm)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_proG1090::GFPint-ADHterm
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. JAB2964

    Plasmid

    #190398

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (6XAmtRO-2XPhlFO::TetR-NLS-19St)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    6XAmtRO-2XPhlFO::TetR-NLS-19St
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. JAB2193

    Plasmid

    #190357

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XTetRO-min35S::GFPint-ADHt)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_6XTetRO-min35S::GFPint-ADHt
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. JAB2178

    Plasmid

    #190351

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XIcaRO-min35S::GFPint-ADHt)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_6XIcaRO-min35S::GFPint-ADHt
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. JAB2177

    Plasmid

    #190350

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-min35S::GFPint-ADHt)

    Depositor

    Jennifer Brophy, Jose Dinneny

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-min35S::GFPint-ADHt
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. L0-I26 pUBQ10 GG0-GG1

    Plasmid

    #195902

    Purpose

    Level 0 pUBQ10 promoter with GG0 and GG1 golden gate linkers

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    pUBQ10 (UBQ10 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET_His6-MBP-TEV-Cas6-RT-Cas1_WT

    Plasmid

    #186274

    Purpose

    Expresses Thiomicrospira Cas6-RT-Cas1 in E. coli

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicropira Cas6-RT-Cas1
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBA1031

    Plasmid

    #189584

    Purpose

    EdH4gp0214 deletion HR vector 250bp HR Arms (EdH4 phage editing)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    EdH4gp0214 Deletion Locus 250bp Homology Arms
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Encodes for a full deletion of EdH4gp0214

    Promoter

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBA1030

    Plasmid

    #189582

    Purpose

    EdH4gp0004 deletion HR vector 250bp HR Arms (EdH4 phage editing)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    EdH4gp0004 Deletion Locus 250bp Homology Arms
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Encodes for a full deletion of EdH4gp0004

    Promoter

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCJH026_proD_Cas12c-multiplexing_CAM

    Plasmid

    #183073

    Purpose

    Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Expressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJCC_084 Cas12i1 D647A

    Plasmid

    #171671

    Purpose

    For bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    Cas12i1 D647A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed aspartate 647 to alanine

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p2CT-His-MBP-Lbu_C2c2_K310A

    Plasmid

    #91856

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbuCas13a (LbuC2c2) K310A
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    K310A

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p2CT-His-MBP-Lbu_C2c2_D1078A

    Plasmid

    #91860

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbuCas13a (LbuC2c2) D1078A
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    D1078A

    Promoter

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p2CT-His-MBP-Lbu_C2c2_N314A

    Plasmid

    #91858

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbuCas13a (LbuC2c2) N314A
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    N314A

    Promoter

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p2CT-His-MBP-Lbu_C2c2_K1080A

    Plasmid

    #91862

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbuCas13a (LbuC2c2) K1080A
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    K1080A

    Promoter

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONR-IAA6

    Plasmid

    #83869

    Purpose

    Gateway donor vector for IAA6

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA6 (IAA6 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDONR-IAA7_no stop

    Plasmid

    #83872

    Purpose

    Gateway donor vector for IAA7 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA7 (IAA7 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDONR-IAA3

    Plasmid

    #83862

    Purpose

    Gateway donor vector for IAA3

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA3 (SHY2 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR-IAA20_no stop

    Plasmid

    #83859

    Purpose

    Gateway donor vector for IAA20 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA20 (IAA20 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDONR-IAA19_no stop

    Plasmid

    #83857

    Purpose

    Gateway donor vector for IAA19 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA19 (IAA19 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDONR-IAA5

    Plasmid

    #83867

    Purpose

    Gateway donor vector for IAA5

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    NP_173011.1 (IAA5 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDONR-IAA31_no stop

    Plasmid

    #83865

    Purpose

    Gateway donor vector for IAA31 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    NP_188387.1 (IAA31 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDONR-IAA2_no stop

    Plasmid

    #83858

    Purpose

    Gateway donor vector for IAA2 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA2 (IAA2 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDONR-IAA19

    Plasmid

    #83856

    Purpose

    Gateway donor vector for IAA19

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA19 (IAA19 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDONR-IAA14_no stop

    Plasmid

    #83852

    Purpose

    Gateway donor vector for IAA14

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA14 (IAA14 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDONR-IAA14

    Plasmid

    #83851

    Purpose

    Gateway donor vector for IAA14

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA14 (IAA14 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDONR-IAA13_no stop

    Plasmid

    #83850

    Purpose

    Gateway donor vector for IAA13 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA13 (IAA13 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDONR-IAA12_no stop

    Plasmid

    #83849

    Purpose

    Gateway donor vector for IAA12 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    NM_100334.4 (IAA12 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. L0-I35 p35S-XVE-pEstradiol GG0-GG1

    Plasmid

    #195904

    Purpose

    Level 0 inducibile estradiol promter and XVE activator protein (p35S-XVE gene-tRBCS-tNOS-LexA-p35S with GG0 and GG1 golden gate linkers)

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    p35S-XVE-LexA-p35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pJRH-1362 scFv entry plasmid

    Plasmid

    #201912

    Purpose

    Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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42. EGFP-GalT

    Plasmid

    #11929

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Cole et al Science. 1996 Aug 9. 273(5276):797-801.

    Insert

    GalT (GALT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amino acids 1-60 of the human galactosyltransfera…

    Promoter

    Available since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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43. pJRH-1179 U6-reci Gag-Cas9 v2

    Plasmid

    #201914

    Purpose

    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Gag-Cas9 v2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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44. pJRH-1180 U6-reci Gag-pol v2

    Plasmid

    #201915

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Gag-pol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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45. E-cadherin-GFP

    Plasmid

    #28009

    Depositor

    Jennifer Stow

    Article

    Miranda et al J Biol Chem. 2001 Jun 22. 276(25):22565-

    Insert

    E-cadherin (CDH1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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46. pAT-WT-Myc

    Plasmid

    #177717

    Purpose

    ER protein alpha-1-antitrypsin (WT), myc tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    alpha-1-antitrypsin (SERPINA1 Human)
    

    Use

    Tags

    Myc

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. pACYC-TtCas6-4xcrRNA4.5

    Plasmid

    #127764

    Purpose

    For expression of T. thermophilus Csm complex in E. coli (crRNA)

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

    Inserts

    Synthetic CRISPR array containing four identical T. thermophilus spacers
    Cas6A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E. coli

    Promoter

    T7

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. pEGFP-VSVG

    Plasmid

    #11912

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

    Insert

    VSVG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F64L, S65T, A206K relative the wild type GFP sequ…

    Promoter

    Available since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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49. pJRH029

    Plasmid

    #171060

    Purpose

    Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Insert

    Gag-spyCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    2x SV40 NLS and 3x FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCAT009

    Plasmid

    #171635

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-B2M
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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