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pLOX-Ttag-iresTK

Plasmid

#12246

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

SV40 Large T, HSV1-TK (SV40gp6 Simian virus)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP1-S127A

Plasmid

#17790

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein mutant S127A (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Serine 127 to Alanine

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

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Integrin beta 2 - mYFP

Plasmid

#8638

Depositor

Timothy Springer

Article

Kim et al Science 2003 Sep 19;301(5640):1720-5.

Insert

Integrin beta 2 (ITGB2 Human)

Tags

YFP

Expression

Mammalian

Mutation

stop codon of beta2 was replaced with AgeI restri…

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only

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pHAGE EF1α dCas9-VP64

Plasmid

#50918

Purpose

Constitutive dCas9-VP64 lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and VP64

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1alpha

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-beta-3

Plasmid

#27289

Depositor

Timothy Springer

Article

Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

Insert

integrin beta 3 (ITGB3 Human)

Tags

His and Myc

Expression

Mammalian

Mutation

n/a

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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WT NFAT2

Plasmid

#11101

Depositor

Anjana Rao

Article

Monticelli et al Eur J Immunol. 2002 Oct . 32(10):2971-8.

Insert

NFAT2 (Nfatc1 Mouse)

Use

Retroviral

Tags

3xHA and GFP

Expression

Mammalian

Available Since

April 19, 2006

Availability

Academic Institutions and Nonprofits only

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pHR-FUSN-mCh-Cry2WT

Plasmid

#101223

Purpose

FUS(1-214) fused to mCh-Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

FUS(aa1-214) (FUS Human)

Use

Lentiviral

Tags

mCherry-Cry2

Expression

Mammalian

Promoter

SFFV

Available Since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-ATF4 mScarlet NLS

Plasmid

#115969

Purpose

ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I

Depositor

David Andrews

Article

Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

Insert

activating transcription factor 4 (ATF4 Human, Synthetic)

Use

Lentiviral

Tags

HA tag, c-myc NLS, and mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE-zeo largeTcDNA

Plasmid

#1779

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

large T antigen (SV40gp6 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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EF.PGK.GFP

Plasmid

#17618

Depositor

Linzhao Cheng

Article

Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pHIV-Zsgreen

Plasmid

#18121

Depositor

Bryan Welm, Zena Werb

Article

Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

Insert

Zsgreen

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pLV_hEF1a_rtTA3

Plasmid

#61472

Purpose

3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

Depositor

Ron Weiss

Article

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Insert

rtTA3

Use

Lentiviral

Promoter

hEF1a

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pminiTol2

Plasmid

#31829

Purpose

Vector with minimal Tol2 transposition sites, with capacity for delivering larger gene cargo than standard Tol2 vectors

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Type

Empty backbone

Use

Cloning vector

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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EGFP-GalT

Plasmid

#11929

Depositor

Jennifer Lippincott-Schwartz

Article

Cole et al Science. 1996 Aug 9. 273(5276):797-801.

Insert

GalT (GALT Human)

Expression

Mammalian

Mutation

Amino acids 1-60 of the human galactosyltransfera…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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HA-HIF2alpha-P405A/P531A-pcDNA3

Plasmid

#18956

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA-ctrl

Plasmid

#85741

Purpose

shRNA ctrl

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

shRNA ctrl

Use

AAV

Tags

EYFP

Available Since

April 22, 2017

Availability

Academic Institutions and Nonprofits only

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JD633

Plasmid

#160393

Purpose

CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Inserts

ZmUbi::wheat GRF4-GIF1 chimera
ZmUbi:TaCas9 + TaU6:gRNA

Use

Binary vector

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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CAG-GFP-IRES-CRE

Plasmid

#48201

Purpose

retroviral vector encoding for GFP and Cre-recombinase

Depositor

Fred Gage

Article

Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

Inserts

eGFP
Cre

Use

Retroviral

Expression

Mammalian

Promoter

CAG and linked by IRES

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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Plasma membrane-targeted optoPLD

Plasmid

#140114

Purpose

Encodes CRY2-mCherry-PLD-P2A-CIBN-CAAX for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-BI30

Plasmid

#183749

Purpose

AAV-BI30 Rep-Cap plasmid for production of AAV-BI30, a capsid with tropism for CNS endothelial cells.

Depositor

Ben Deverman

Article

Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

Insert

AAV9 modified with 7mer insertion between amino acids 588 and 589 of VP1

Use

AAV

Mutation

K449R, and NNSTRGG inserted between 588 and 589 o…

Promoter

p41

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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