We narrowed to 5,784 results for: pcas

1. pCAG-memRFP

   Plasmid

   #224564

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and membrane RFP reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   mRFP1
   

   Use

   Unspecified

   Tags

   Membrane localization signal

   Mutation

   Codon optimized

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG_mRuby2_smFP OLLAS

   Plasmid

   #59761

   Purpose

   encodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   mRuby2_smFP_OLLAS
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-Beatrix

   Plasmid

   #167528

   Purpose

   Dual-color fluorescent reporter for the creation and detection of Cre-dependent tunable mosaics in floxed mouse lines and cells.

   Depositor

   Gian Michele Ratto

   Article

   Trovato et al Nat Commun. 2020 Dec 3;11(1):6194. doi:

   Insert

   Beatrix
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. AB65_pCAG.Cas9-D10A.rBGpA

   Plasmid

   #199256

   Purpose

   Expression construct encoding the SpCas9-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   SpCas9.D10A nickase
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEC1.2/EC1.1_spCas9_TagRFP

   Plasmid

   #161934

   Purpose

   CRISPR plasmid with spCas9 expressed under egg cell promoter - TagRFP seed selection

   Depositor

   Christian Fankhauser

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-sfGFP

   Plasmid

   #125747

   Purpose

   sfGFP without iCreERT2 fusion

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   sfGFP
   

   Expression

   Mammalian

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459_gRNA-ROSA26_hspCas9

   Plasmid

   #193310

   Purpose

   Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   not applicable

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9-mSA*

   Plasmid

   #182952

   Purpose

   Express SpCas9-mSA*

   Depositor

   Wen Xue

   Article

   Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

   Insert

   SpCas9-mSA*
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG/ATP10D-HA

   Plasmid

   #209217

   Purpose

   Mammalian expression of ATP10D

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

   Insert

   ATP10D (ATP10D Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-Positron

    Plasmid

    #129253

    Purpose

    Express Positron in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Positron
    

    Expression

    Mammalian

    Mutation

    D92N, E199V

    Promoter

    CAG

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. B-Sniper SpCas9

    Plasmid

    #207361

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Sniper SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    F539S, M763I, K890N, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG/ATP11A-HA

    Plasmid

    #209218

    Purpose

    Mammalian expression of ATP11A

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP11A (ATP11A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAP-BAC

    Plasmid

    #120229

    Purpose

    TAR cloning in yeast and low-copy maintenance of large constructs in E. coli

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS_cAMPFIRE-L-R279E

    Plasmid

    #182286

    Purpose

    Mammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
    

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-PDZD8HA

    Plasmid

    #105005

    Purpose

    Mammalian Expression of PDZD8

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    PDZD8 (Pdzd8 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCambia-loxKan

    Plasmid

    #199590

    Purpose

    basic plant vector with a plant-selectable kanamycin resistance cassette flanked by both loxP and FRT sites for sustainable plant engineering with subsequent marker removal possibility

    Depositor

    Andreas Stuermer

    Insert

    FRT-lox66-NosP-KanR-NosT-lox71-FRT
    

    Use

    Cre/Lox

    Expression

    Plant

    Promoter

    Nos

    Available Since

    July 5, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCAG-hHDAC6

    Plasmid

    #21099

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    Histone Deacetylase 6 (HDAC6 Human)
    

    Expression

    Mammalian

    Available Since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pCAG-HcRed

    Plasmid

    #11152

    Purpose

    Mammalian expression vector for expression of HcRed

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Insert

    HcRed1 from Heteractis crispa
    

    Expression

    Mammalian

    Available Since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. Topo_SpCas9.tracr_AsCas12a.DR

    Plasmid

    #155050

    Purpose

    TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
    

    Use

    CRISPR

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-B19G

    Plasmid

    #59921

    Purpose

    Expression vector for Rabies virus SAD B19 glycoprotein gene

    Depositor

    Ian Wickersham

    Insert

    Rabies virus glycoprotein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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