Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 63,680 results for: cloning

Clear keywords
Showing: 5181 - 5200 of 63680 results

  3. pcDNA5/FRT/TO-GAlphai3-RLuc8

    Plasmid
    #140975
    Purpose
    Encodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAlphai3-RLuc8 (GNAI3 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140975-plasmid-map-sequence-id-300658

    Enlarge

  8. YEE-BE4max

    Plasmid
    #138157
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(YEE)-nSpCas9-UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 W90Y + R126E + R132E; within Cas9…
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138157-plasmid-map-sequence-id-272348

    Enlarge

  9. pJC49

    Plasmid
    #133060
    Purpose
    Lentiviral vector with a (Ubiquitous Chromatin Opening Element) UCOE upstream of a CAG promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mScarlet-I fusion protein.
    Depositor
    Martin Kampmann
    Publication
    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094
    Insert
    Tau.K18(LM)
    Use
    Lentiviral
    Tags
    mScarletI
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133060-plasmid-map-sequence-id-271174

    Enlarge

  16. pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)

    Plasmid
    #130640
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.
    Depositor
    Samuel H. Sternberg
    Publication
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6
    Use
    CRISPR; Transposon
    Tags
    His10-MBP-TEVsite on TniQ
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130640-plasmid-map-sequence-id-255648

    Enlarge

  17. pDEST-CMV mCherry-GFP-LC3B G120A

    Plasmid
    #123235
    Purpose
    Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.
    Depositor
    Robin Ketteler
    Publication
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Mutation
    Glycine 120 to Alanine
    Promoter
    CMV
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123235-plasmid-map-sequence-id-378779

    Enlarge

  19. pLenti- V6.3 Ultra-Chili

    Plasmid
    #106173
    Purpose
    lentiviral CMV driven tdTomato-P2A-T2A, Blasticidin selectable
    Depositor
    Ewa Snaar-Jagalska
    Insert
    dTomato
    Use
    Lentiviral
    Tags
    T2A, P2A
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    106173-plasmid-map-sequence-id-207442

    Enlarge

  20. LV-Sox10MCS5-GFP-EF1a-mCherry

    Plasmid
    #115782
    Purpose
    Sox10-MCS5-GFP and a constitutive EF1α-mCherry reporter plasmid. Construct generates constitutive red fluorescence and basal promoter cfos conjugated SOXMCS5 enhancer driven GFP expression in a cell.
    Depositor
    Fraser Sim
    Publication
    Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1
    Inserts
    mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
    EF1alpha promoter driven constitutive mCherry
    Use
    Lentiviral
    Tags
    EF1α promoter fused to mCherry and cfos promoter …
    Promoter
    SOX10 Multiple Species Conserved enhancer element…
    Available since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    115782-plasmid-map-sequence-id-226932

    Enlarge

Showing: 5181 - 5200 of 63680 results