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162,160 results

Showing: 7801 - 7830 of 162160 results
  1. pRPF215

    Plasmid
    #106377
    Purpose
    C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon
    Insert
    Ptet-himar1-Ter(slpA)-transposon
    Use
    E. coli - c. difficile shuttle vector
    Tags
    Expression
    Mutation
    Codon optimised for C. difficile
    Promoter
    Ptet
    Available since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pSLIK_hygro_3xflag_NRF2(RR)

    Plasmid
    #136535
    Purpose
    Lentiviral expression vector for an inducible 3xflag-tagged NRF2(a1584c_a1586t_a1589g)
    Depositor
    Insert
    NRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)
    Use
    Lentiviral; Destinatioin vector for gateway cloni…
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    This plasmid is developed by mutating 3 base pair…
    Promoter
    Available since
    July 1, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pSynSRE-T-Luc

    Plasmid
    #60444
    Purpose
    Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.
    Depositor
    Insert
    HMG-CoA synthase promoter (-324/-225)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    HMG-CoA synthase (-324/-225) & TATAA (-28/+39)
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA3.1-N-Luc

    Plasmid
    #185357
    Purpose
    For mammalian expression of N-terminal half of Firefly luciferase for split-Luciferase experiments (amino acids 2-416).
    Depositor
    Insert
    Firefly Luciferase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pIRES-EGFP-puro

    Plasmid
    #45567
    Depositor
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    IRES-EGFP-Puro
    Expression
    Mammalian
    Mutation
    Promoter
    Phcmv (strong human cytomegalovirus immediate ear…
    Available since
    June 14, 2013
    Availability
    Academic Institutions and Nonprofits only
  6. pCS Omomyc-Mer

    Plasmid
    #113170
    Purpose
    Expression of Omomer (tamoxifen inducible Omomyc) in mammalian cells
    Depositor
    Insert
    Omomer: a fusion contruct of Omomyc and MER (a mutant estrogen receptor, reponsive to tamoxifen) (MYC Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E57T, E64I, R70Q, R71N
    Promoter
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. pT3-EF1aH

    Plasmid
    #180149
    Purpose
    This plasmid is an empty vector used as the control for other plasmids which are in pT3-EF1aH backbone. It does not have any restriction site for cloning.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pICE-FLAG-NAT10-siR-G641E

    Plasmid
    #59366
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of G641E mutant of human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Insert
    NAT10 NM_024662 (NAT10 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    G641E and silent mutations to render the cDNA res…
    Promoter
    CMV-tet
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
  9. pLenti Lifeact-mRuby2 PuroR

    Plasmid
    #84386
    Purpose
    Lentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neurons
    Depositor
    Insert
    Lifeact
    Use
    Lentiviral
    Tags
    mRuby2
    Expression
    Mutation
    Promoter
    CMV immediate early
    Available since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
  10. pCBC-DT3T4

    Plasmid
    #50592
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Insert
    T3, gRNA scaffold, U6-1tplus, U6-26p, T4
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  11. pJMP12042

    Plasmid
    #222354
    Purpose
    Tn7 integrative vector with PabstBR promoter expression cassette and aprR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. pHRIG-Akt3

    Plasmid
    #53596
    Purpose
    lentiviral expression of constitutively active human Akt3 and EGFP
    Depositor
    Insert
    cAkt3 (AKT3 Human)
    Use
    Lentiviral
    Tags
    HA and IRES-EGFP
    Expression
    Mammalian
    Mutation
    constitutively active (myristoylated version)
    Promoter
    CMV
    Available since
    Aug. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 7801 - 7830 of 162160 results