We narrowed to 11,800 results for: emb
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Plasmid#138234PurposeExpresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only
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pL2-GGA(EAAAK)2AGG
Plasmid#137104PurposeiFLinkC Linker cloningDepositorInsertGGA(EAAAK)2AGG
UseSynthetic BiologyAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSP64-H2B-GFP
Plasmid#135605PurposeExpression of H2B-GFP mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B-GFP
UseIn vitro transcription (mrna synthesis)TagsGFPPromoterUnknownAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPagP
Plasmid#112125PurposeBacterial expression of outer membrane protein PagP, wild typeDepositorInsertPagP (pagP E. coli)
ExpressionBacterialMutationN-terminal signal sequence not included and repla…PromoterT7Available sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-PH-citrine
Plasmid#131406PurposePurpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrineDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPH
UseIn vitro transcriptionTagscitrine yellow fluorescent proteinPromoterT7Available sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLSU4mg10
Plasmid#126079PurposePlant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cellsDepositorInsertmitochondria Rieske iron sulphur protein (1-100 aa)
UseBinaryTagsGFP1-10ExpressionPlantPromoter'long' CaMV35SAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
sgMAPKAP1_1
Plasmid#124912PurposeTargeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9DepositorInsertMAPKAP1 (MAPKAP1 Human)
UseCRISPR and LentiviralAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Arl6ip6
Plasmid#120235PurposeExpresses V5-tagged Arl6ip6 in lentiviral vectorDepositorAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rap1b E63
Plasmid#118328PurposeExpression of bovine Rap1bDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRap1b E63 (RAP1B Bovine)
TagsGFPExpressionMammalianMutationQ63EAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TetO-U2B''-fSNAPc
Plasmid#106988Purposeopen reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sitesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUB2" (SNRPB2 Human)
TagsfSNAPAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
YFP-HA-DAT W63A
Plasmid#90246PurposeExpresses YFP-HA-DAT W63A mutant in mammalian cellsDepositorInsertDAT (dopamine transporter) (SLC6A3 Human)
ExpressionMammalianMutationinserted HA tag (see YFP-HA-DAT), mutated W63 to APromoterCMVAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
YFP-HA-DAT R60A
Plasmid#90245PurposeExpresses YFP-HA-DAT R60A mutant in mammalian cellsDepositorInsertDAT (dopamine transporter) (SLC6A3 Human)
ExpressionMammalianMutationinserted HA tag (see YFP-HA-DAT), mutated R60 to APromoterCMVAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GST-RP2
Plasmid#86072PurposeA bacterial expression plasmid encoding retinitis pigmentosa 2 (RP2) with N-terminus GST-tag.DepositorAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-FRB:Akt1(D274A)
Plasmid#86634PurposeExpresses FRB-tagged human Akt1(D274A) kinase-inactive mutantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFRB-Akt1(D274A) (AKT1 Human)
ExpressionMammalianAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Dicer_1
Plasmid#68807Purposespecific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.DepositorInsertsgRNA mouse Dicer1
UseCRISPR and Mouse TargetingExpressionBacterial and MammalianAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGB2alpha2 35s:hCas9:tNos (GB0639)
Plasmid#68222PurposeTranscriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoterDepositorInserthCas9
UseCRISPR and Synthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-DH
Plasmid#67898PurposeExpresses a fusion of mCherry with the DH domain (155-347) of p63RhoGEF in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-DH
ExpressionMammalianPromoterCMVAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET15b+PCNA1 WT
Plasmid#66139PurposeExpresses the wild type of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS1011
Plasmid#63783PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Strep tag II followed by TRP1 selection markerDepositorPublicationInsertStrep-tag II followed by TRP1 selection marker
UseE. coli cloning vectorTagsStrep-tag IIPromoternoneAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
TAPBPL pEGFP-C3 (1765)
Plasmid#62055Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only