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Flrt3-Fc-His

Plasmid

#72075

Purpose

Expresses the extracellular region of the FLRT3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Flrt3 (Flrt3 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pHR KIF17 (1-738)

Plasmid

#60406

Purpose

Expression of truncated form of human homodimeric kinesin-2 (KIF17) amino acids 1-738

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

KIF17 (KIF17 Human)

Use

Lentiviral

Tags

GB1, Strep-Tag, and sfGFP

Expression

Mammalian

Mutation

Truncated version amino acids 1-738

Promoter

SFFV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only

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3xFNLDD/pMXs-IG

Plasmid

#48972

Purpose

Expresses 3xFNLDD in mammalian cells

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2015 Jan 21;10(1):e0116579. do

Insert

3xFNLDD

Use

Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Promoter

LTR

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pEF1a-myc-LRH1(ES)-IRES-neo

Plasmid

#28092

Depositor

Austin Cooney

Article

Wagner et al Stem Cells. 2010 Oct . 28(10):1794-804.

Insert

Lrh-1 (Nr5a2 Mouse)

Tags

myc

Expression

Mammalian

Available Since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-myc3-CUL4A delta N100

Plasmid

#19953

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CUL4A (cullin 4A) (CUL4A Human)

Tags

myc3

Expression

Mammalian

Mutation

The longest published clone (Q13619)

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pFLAG-4EHP-mISG15

Plasmid

#17355

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

4EHP-mISG15 (EIF4E2 Human, Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only
AiP12556 - pAAV-AiE0410m_3xC-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2556)

Plasmid

#208144

Purpose

AiE0410m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain oligodendrocytes

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp10

Plasmid

#156479

Purpose

Bacterial expression of Sars-CoV2 Nsp10 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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CF-PIPK

Plasmid

#20157

Depositor

Tobias Meyer

Article

Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

Insert

CFP-FKBP-PIPK (Pip5k1c Mouse)

Expression

Mammalian

Mutation

C-terminus (amino acids 636-661)truncated, R445E,…

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-PHGDH

Plasmid

#74113

Purpose

Expression of N-terminal His-tagged

Depositor

Lewis Cantley

Article

Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

PHGDH (PHGDH Human)

Tags

His-tagged

Expression

Bacterial

Promoter

T7 promoter

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTU2-A-RFP (colE1 origin)

Plasmid

#74093

Purpose

pTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replication

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Golden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication

Use

Synthetic Biology

Expression

Bacterial

Promoter

R0010 Const. Promoter controlling RFP

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG_huMcl-1.1

Plasmid

#85530

Purpose

Inducible expression of guide RNA (huMcl-1.1) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

hu Mcl-1.1 (MCL1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1t

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Sema7a-Fc-His

Plasmid

#72175

Purpose

Expresses the extracellular region of the Sema7A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema7a (Sema7a Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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dmBACCS2VL-IRES-dOrai

Plasmid

#67629

Purpose

Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2VL-IRES-dOrai

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pECE-M2-AMPKalpha2 M93G

Plasmid

#31653

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)

Tags

M2

Expression

Mammalian

Mutation

changed Methionine 93 to Glycine

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
E pCMV-CFPLoxP-YFPatt

Plasmid

#24509

Depositor

Jehonathan Pinthus

Article

Lu et al Nat Prec (2008).

Insert

ECFP, EYFP

Tags

ECFP and EYFP

Expression

Mammalian

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE intramol-1u

Plasmid

#13549

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIP-E intramol WT (gltI E. coli)

Tags

EYFP

Expression

Bacterial

Mutation

ECFP insert in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pCI-Neo-gp78/2853

Plasmid

#11435

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

gp78 (AMFR Human)

Expression

Mammalian

Mutation

n/a

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p-PDI

Plasmid

#202486

Purpose

Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Human, Budding Yeast)

Expression

Bacterial

Promoter

EM7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

Plasmid

#188765

Purpose

dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

dCas9-XTEN80-KRAB(Kox1)

Use

Lentiviral

Tags

HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Promoter

SFFV

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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