159,352 results

1. pAAV.hSynap.iGABASnFR.F102G

   Plasmid

   #112160

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Insert

   iGABASnFR.F102G
   

   Use

   AAV

   Tags

   Expression

   Mutation

   F102G

   Promoter

   hSynapsin

   Available since

   July 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMXs-c-Myc-T58A

   Plasmid

   #13372

   Purpose

   Retroviral expression of mouse c-Myc T58A mutant

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   myelocytomatosis oncogene (T58A mutant) (Myc Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Tyrosine 58 to Alanine

   Promoter

   Available since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV.hSyn.eGFP.WPRE.bGH

   Plasmid

   #105539

   Purpose

   AAV expression of EGFP from hSyn promoter

   Depositor

   James M. Wilson

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. K44A dynamin 1 pEGFP

   Plasmid

   #34681

   Depositor

   Sandra Schmid

   Article

   Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

   Insert

   dynamin 1ba (DNM1L Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K44A

   Promoter

   Available since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-HA-Ebp1

   Plasmid

   #67792

   Purpose

   Mammalian expression of Ebp1 tagged with HA

   Depositor

   Stephen Goff

   Article

   Wang et al J Virol. 2014 Feb;88(3):1825-9. doi: 10.

   Insert

   Ebp1 (PA2G4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pHR SFFVp PHAkt Cerulean

   Plasmid

   #50837

   Purpose

   Fluorescent reporter for membrane PIP3 levels

   Depositor

   Wendell Lim

   Article

   Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

   Insert

   PHAkt (AKT1 )
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Cerulean

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMApra-Para

   Plasmid

   #218362

   Purpose

   Empty backbone with araC-ParaBAD expression system

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJH2685

   Plasmid

   #191372

   Purpose

   Punc-4-TeTx::mCherry unc-54 3' UTR C.elegans A MNs/others neurou expression of TeTx RFP

   Depositor

   Mei Zhen

   Article

   Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

   Insert

   Tetanus Toxin Light Chain
   

   Use

   Tags

   wCherry

   Expression

   Worm

   Mutation

   Promoter

   Punc-4

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pXPR_047

   Plasmid

   #107145

   Purpose

   measures Cas9 activity

   Depositor

   David Root

   Insert

   GFP + sgRNA for GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223-PLXNA3

    Plasmid

    #23518

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PLXNA3 (PLXNA3 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    L1655M

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pEGFP-VSP4-E228Q

    Plasmid

    #80351

    Purpose

    Expression of GFP-tagged, dominant negative VPS4 mutant

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    VPS4-E228Q (VPS4A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRSET FLIPPi-30m

    Plasmid

    #13556

    Depositor

    Wolf Frommer

    Article

    Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

    Insert

    FLIPPi T22A
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    T22A in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

    Plasmid

    #67979

    Purpose

    Cas9 activity reporter (control) with BFP and GFP.

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTriEx-NTOM20-LOV2

    Plasmid

    #81009

    Purpose

    Expression of LOV2 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV2
    

    Use

    Tags

    NTOM20

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV, p10

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

    Plasmid

    #128424

    Purpose

    Human Codon Optimized dCas9-DNMT3A3L

    Depositor

    Bradley Bernstein

    Article

    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

    Insert

    dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7 Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHR-dSV40-CTLA-4-mGFP

    Plasmid

    #223594

    Purpose

    Lentiviral expression of human CTLA4 with mGFP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    mGFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. VPS13D^EGFP

    Plasmid

    #174109

    Purpose

    The insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Codon optimized VPS13D
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    With an internal EGFP tag in aminoacid position 1…

    Promoter

    CMV

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. dCas9-10xSunTag-P2A-tagBFP

    Plasmid

    #174141

    Purpose

    Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    dCas9, 10xSunTag, tagBFP
    

    Use

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BFP-Sec61 beta

    Plasmid

    #49154

    Purpose

    expression of Sec61 beta fused to BFP, used as a general ER marker

    Depositor

    Gia Voeltz

    Article

    Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

    Insert

    Sec61 beta (SEC61B Human)
    

    Use

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-N1-Tspan4

    Plasmid

    #210323

    Purpose

    Expresses Tetraspanin-4 (TSPAN4) as migrasome marker

    Depositor

    Li Yu

    Article

    Ma et al Cell Res. 2015 Jan;25(1):24-38. doi: 10.

    Insert

    Tetraspanin 4 (Tspan4 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGL3 1 kb prom and exon1 fragment CD274

    Plasmid

    #107006

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. lis-1::L4440

    Plasmid

    #11336

    Depositor

    Guy Caldwell

    Insert

    lis-1 (lis-1 Nematode)
    

    Use

    RNAi

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

23. pDONR221_SLC39A6

    Plasmid

    #132153

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC39A6 (SLC39A6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213027

    Purpose

    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    D702Y (RT- reverse transcriptase catalytic dead)

    Promoter

    CMV

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MXS_CMV::ZeoR-bGHpA

    Plasmid

    #62441

    Purpose

    MXS_chaining vector with CMV::ZeoR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against Zeocin with CMV Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLL3.7

    Plasmid

    #11795

    Purpose

    3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.

    Depositor

    Luk Parijs

    Article

    Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti6.2_mCherry_Grx1_roGFP2

    Plasmid

    #155045

    Purpose

    Fluorescent redox reporter

    Depositor

    Vanessa LaPointe

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3 Flag-humanTRAF6 (1093)

    Plasmid

    #66929

    Purpose

    Mammalian Expression of TRAF6

    Depositor

    Michael Karin

    Insert

    TRAF6 (TRAF6 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCR-XL-rAkap9

    Plasmid

    #196867

    Purpose

    Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Akap9 (Akap9 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac promoter and T7 promoter

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-HA-CCND3

    Plasmid

    #172621

    Purpose

    Expresses HA-tagged cyclin D3 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge