164,220 results

1. pCDH-3xFLAG-hTCAB1-puroR

   Plasmid

   #167459

   Purpose

   3rd-gen lentiviral expression of FLAG-TCAB1 (WDR79) in mammalian cells

   Depositor

   Steven Artandi

   Article

   Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

   Insert

   TCAB1 (WRAP53 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTHSSe_42

   Plasmid

   #109240

   Purpose

   pSC101 BbsI cloning vector

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23105

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. CROPseq-3P5P

   Plasmid

   #219680

   Purpose

   CROPseq vector enabling gRNA detection via mRNA-based capture (CROPseq), 3'-based direct capture using 10x capture sequence 1 or 5'-based enrichment using a custom reverse transcription primer

   Depositor

   Christoph Bock

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDUP 4-1

   Plasmid

   #23022

   Depositor

   Douglas Black

   Article

   Modafferi et al Mol Cell Biol. 1997 Nov . 17(11):6537-45

   Insert

   c-src (Src Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAVS1-TLR targeting vector

   Plasmid

   #64215

   Purpose

   Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Insert

   traffic light reporter (TLR) (AAVS1 Human)
   

   Use

   Human targeting

   Tags

   Expression

   Mutation

   see publication for details

   Promoter

   CAG

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. C1QBP-bio-His

   Plasmid

   #53330

   Purpose

   Expresses full-length Complement component 1 Q subcomponent-binding protein ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   C1QBP (C1QBP Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. tdTomato-Zyxin-6

   Plasmid

   #58148

   Purpose

   Localization: Focal Adhesions, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Article

   Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

   Insert

   Zyxin (ZYX Human)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

8. 35S-GAL4BD-GWY/pAM

   Plasmid

   #201233

   Purpose

   Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

   Depositor

   Michael Ayliffe

   Article

   Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

   Insert

   35S-GAL4BD-GWY
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. ATP1A1_G4_Q118R_Std_Dual_epegRNA_tevopreQ1

   Plasmid

   #187454

   Purpose

   Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G4 Q118R pegRNA (ATP1A1 Human)
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available Since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV mRuby3-Synapsin1a

    Plasmid

    #187896

    Purpose

    For labeling pre-synaptic terminals

    Depositor

    Timothy Ryan

    Insert

    Synapsin1a
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-CRY2oligo-mcherry-ANXA11

    Plasmid

    #164203

    Purpose

    Expresses CRY2oligo-mcherry tagged ANXA11 under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Tags

    CRY2oligo-mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2-SauCas9

    Plasmid

    #174040

    Purpose

    In vitro transcription of SauCas9 mRNA

    Depositor

    James Gagnon

    Article

    Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

    Insert

    SauCas9
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRY2PHR-mCherryN1

    Plasmid

    #26866

    Purpose

    Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2PHR (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. plentiCMV-sct-pp65-b2m-HLA-A0201

    Plasmid

    #196511

    Purpose

    Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    single chain of a pp65 peptide, b2m and HLA A0201
    

    Use

    Lentiviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Y84C

    Promoter

    CMV

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)

    Plasmid

    #197510

    Purpose

    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
    

    Use

    AAV and CRISPR

    Tags

    BPNLS and NpuN(intein)-BPNLS

    Expression

    Mutation

    ABE8e mutations in TadA and nSpRY(D10A/A61R)

    Promoter

    Cbh

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid

    #122650

    Purpose

    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.

    Depositor

    Howard Hang

    Article

    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

    Insert

    PylRS-AF, Pyl-tRNACUA
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…

    Promoter

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-Rab7A

    Plasmid

    #61803

    Purpose

    Human Rab7A fluorescently tagged with GFP on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Tags

    2x HA and GFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSF-KpAGO207-1251

    Plasmid

    #117854

    Purpose

    Expressed KpAGO 207-1251 in E. coli under IPTG-inducible promotor

    Depositor

    Dinshaw Patel, Kotaro Nakanishi

    Article

    Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

    Insert

    Argonaute
    

    Use

    Tags

    6xHis-SUMO encoded in vector

    Expression

    Bacterial

    Mutation

    missing first 206 amino acid residues

    Promoter

    T7

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYDS2.0_AT118-L

    Plasmid

    #222847

    Purpose

    Yeast-display plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-L
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSEVA23-GG

    Plasmid

    #208013

    Purpose

    for cloning homology arms, with GFP as counter selection marker

    Depositor

    Pablo Ivan Nikel

    Article

    Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

    Insert

    msfGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    14G

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. eGFP STAT1 S727E

    Plasmid

    #12305

    Depositor

    Alan Perantoni

    Article

    Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

    Insert

    STAT1 S727E (STAT1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S727E

    Promoter

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. human pirh2-(1-261)

    Plasmid

    #24878

    Depositor

    Cheryl Arrowsmith

    Article

    Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

    Insert

    Pirh2 (RCHY1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pEF.myc.ER-E2-Crimson

    Plasmid

    #38770

    Purpose

    In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

    Depositor

    Benjamin Glick

    Article

    Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

    Insert

    E2-Crimson
    

    Use

    Tags

    ER retention signal (KDEL), ER signal sequence (M…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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24. hMLKL-Venus

    Plasmid

    #106078

    Purpose

    expression in mammalian cells

    Depositor

    Douglas Green

    Article

    Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

    Insert

    human MLKL-Venus (MLKL Human)
    

    Use

    Retroviral; Tet-on retrovirus

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Dox-inducible

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCAG-HA-NLGN3 WT

    Plasmid

    #59318

    Purpose

    Expression plasmid of HA-tagged wildtype human Neuroligin 3

    Depositor

    Peter Scheiffele

    Article

    Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

    Insert

    NLGN3 (NLGN3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PcDNA3.1-2Strep

    Plasmid

    #210343

    Purpose

    Empty Vector for molecular cloning

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    2Strep tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Dendra2-CD9-10

    Plasmid

    #57705

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Insert

    CD9 (CD9 Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

28. pCherryGFP-CoV2-WT

    Plasmid

    #177618

    Purpose

    Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting

    Depositor

    Junjie Guo

    Article

    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

    Insert

    SARS-CoV-2 FSE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-ZNF598-TEV-3xFLAG

    Plasmid

    #105690

    Purpose

    Expresses C terminally 3xFLAG tagged ZNF598 protein

    Depositor

    Ramanujan Hegde

    Article

    Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

    Insert

    ZNF598 (ZNF598 Human)
    

    Use

    Tags

    TEV-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (from vector)

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. pFUW-tetO-BCL6

    Plasmid

    #139816

    Purpose

    doxycycline-inducible expression of Human BCL6 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    BCL6 (BCL6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T190A, E241G, L314M- please see depositor comments

    Promoter

    TRE-mCMV

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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