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pcDNA3.2/pr-Membrane

Plasmid

#120817

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

pr-Membrane ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-Rluc-A114-N40

Plasmid

#50561

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Article

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-A114-N40

Use

Cloning vector

Promoter

lac

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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dCas13d-BFP

Plasmid

#215720

Purpose

Catalytically inactive dCasRx with 2A-BFP

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Insert

dCasRx

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-Bsd-CMV-MDM4

Plasmid

#202669

Purpose

MDM4 overexpression vector with blasticidin resistance

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 regulator of p53 (MDM4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-EEA1

Plasmid

#174451

Purpose

Express EGFP with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with early endosome-targeting sequence

Tags

early endosomal antigen 1 (EEA1)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMEH31 Lamp2b HA

Plasmid

#168208

Purpose

Mammalian expression of lysosome-associated membrane protein 2b fused to a C-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

Lamp2b (LAMP2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-PIK3CG

Plasmid

#174189

Purpose

Expresses PIK3CG in mammalian cells

Depositor

Cheng-han Yu

Article

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3CG (PIK3CG Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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MSI2-ADA

Plasmid

#166967

Purpose

Express the fusion protein of human MSI2 and the hyperactive catalytic domain of Drosophila ADAR

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

MSI2-ADA

Use

Retroviral

Tags

MSI2-ADA

Expression

Mammalian

Promoter

MSCV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgBAP1-2

Plasmid

#125838

Purpose

KO BAP1 gene

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMAL-PUM1

Plasmid

#120384

Purpose

Bacterial expression of MBP-Pumillio1 fusion protein

Depositor

Igor Ulitsky

Article

Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

Insert

PUM1 (PUM1 Human)

Tags

6xHIS and MBP

Expression

Bacterial

Promoter

LacI

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pWPI-IRES-eGFP-MEFV

Plasmid

#92103

Purpose

Expression in eukaryotic cells and generating stable cell lines

Depositor

Feng Shao

Article

Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only
pLX_TRC208-SYNJ2BP-V5

Plasmid

#92149

Purpose

Expresses V5-tagged human SYNJ2BP in mammalian cells.

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

SYNJ2BP-V5 (SYNJ2BP Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

*(see below)

Promoter

CMV

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti6-DCX-EGFP

Plasmid

#80599

Purpose

pLenti6 destination vector encoding C-terminally EGFP-tagged human doublecortin (DCX; GenBank: NM_178152)

Depositor

Torsten Wittmann

Article

Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. d

Insert

Doublecortin (DCX Human)

Use

Lentiviral

Tags

EGFP

Promoter

CMV

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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N-term Hec1 Plk1 FRET sensor c-jun substrate

Plasmid

#45204

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Hec1-targeted Plk1 FRET sensor

Tags

CyPET, YPET

Expression

Mammalian

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt9b

Plasmid

#42287

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt9b (Wnt9b Mouse)

Expression

Mammalian

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-mFzd2-1D4

Plasmid

#42264

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 2 (Fzd2 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pCS2 hSmad1-MM

Plasmid

#22990

Depositor

Edward De Robertis

Article

Pera et al Genes Dev. 2003 Dec 15. 17(24):3023-8.

Insert

Smad1 (SMAD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S214A, S206A, S195A, S187A

Available Since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
mPERK

Plasmid

#24826

Depositor

Fumihiko Urano

Article

Ghosh et al PLoS One. 2010 . 5(3):e9575.

Insert

mPERK (Eif2ak3 Mouse)

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available Since

June 30, 2010

Availability

Academic Institutions and Nonprofits only
ZE26 Stbm-N-6myc-pCS2+

Plasmid

#17068

Depositor

Randall Moon

Article

Park et al Nat Cell Biol. 2002 Jan;4(1):20-5.

Insert

Stbm (vangl2 Zebrafish)

Use

Zebrafish expression

Tags

Myc6

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pDONR221-EWSR1 Stop

Plasmid

#84887

Purpose

DONOR vector for Gateway cloning of EWSR1

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway Cloning

Promoter

none

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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