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SpyCatcher002-oTet

Plasmid

#124663

Purpose

Expresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oTet

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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LAMP1-GFP

Plasmid

#16290

Depositor

Ron Vale

Article

Minin et al J Cell Sci. 2006 Feb 15. 119(Pt 4):659-7

Insert

LAMP1/RE72002

Tags

EGFP

Expression

Insect

Promoter

Ac5

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pTUM4

Plasmid

#230905

Purpose

Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli

Depositor

Arne Skerra

Article

Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-

Inserts

FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
SurA (surA E. coli)
DsbA (dsbA E. coli)
DsbC (dsbC E. coli)

Expression

Bacterial

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-42B3mutAC2(Ser2ph)-SNAP

Plasmid

#174876

Purpose

PiggyBac transposon vector for expression of RNAPII Ser2ph mintbody

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

RNAPII Ser2ph mintbody-SNAP

Use

Piggybac vector

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-IGSF8-Fc(DAPA)-AviTag-6xHis

Plasmid

#156912

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

IGSF8 (IGSF8 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pmRFP-LC3

Plasmid

#21075

Purpose

Mammalian expression of rat LC3 fused to mRFP

Depositor

Tamotsu Yoshimori

Article

Kimura et al Autophagy. 2007;3(5):452-60.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

mRFP

Expression

Mammalian

Mutation

First ATG is not included.

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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pTKEI-Mal-B2

Plasmid

#79756

Purpose

Single fluorescent protein biosensor for maltose

Depositor

David Savage

Article

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Insert

Mal-B2

Tags

6xHis

Expression

Bacterial

Promoter

pTrc

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-UbC-Tet1v4-dCas9

Plasmid

#232180

Purpose

Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells

Depositor

Charles Gersbach

Article

Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

Insert

Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 S. pyogenes, Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, H840A

Promoter

hUbC

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oTri

Plasmid

#124662

Purpose

Expresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oTri

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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Human Bcl-2/pcDNA3

Plasmid

#19279

Depositor

Philip Leder

Article

Fantin et al Cancer Res. 2004 Jan 1. 64(1):329-36.

Insert

human Bcl-2 (BCL2 Human)

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pUAS-NanoLuc

Plasmid

#87696

Purpose

Gal4VP16 driven expression of NanoLuc

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

NanoLuc

Tags

nuclear localization signal

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX307-FURIN-puro

Plasmid

#158460

Purpose

Constitutive lentiviral expression of human FURIN.

Depositor

Alejandro Chavez, Sho Iketani

Insert

FURIN (FURIN Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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PB CAG-sfGFP

Plasmid

#133568

Purpose

CAG sfGFP piggyBac transposon

Depositor

Mario Capecchi

Insert

sfGFP

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-WT

Plasmid

#133753

Purpose

Wild type human TDP-43 in pLVX-Puro lentiviral vector

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pet30b-7M SrtA

Plasmid

#51141

Purpose

expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

Depositor

Hidde Ploegh

Insert

S.aureus SrtA 7M

Tags

6x His

Expression

Bacterial

Mutation

P94R, E105K, E108Q, D160N, D165A, K190E, K196T

Promoter

T7

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pgRNA-CKB

Plasmid

#73501

Purpose

gRNA expression vector (with mKate2)

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

mKate2-T2A-Bsd

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pIG-829_HA-GD2-28z_CAR_JUN_Retroviral

Plasmid

#207506

Purpose

This plasmid can be used to generate retrovirus.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

JUN, HA-GD2-28z_CAR (JUN Human)

Use

Retroviral

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti_gRNA-Puro

Plasmid

#84752

Purpose

Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

Depositor

Hyongbum Kim

Article

Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMK243 (Tet-OsTIR1-PURO)

Plasmid

#72835

Purpose

Expresses OsTIR1 under the control of the Tet promoter

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

AAVS1-Tet-OsTIR1-PURO-AAVS1

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-scCAK1

Plasmid

#177014

Purpose

Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)

Tags

Polyhistidine-TEV

Expression

Insect

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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