We narrowed to 22,833 results for: Sis

1. GFP-ATG8(414)/GFP-AUT7(414)

   Plasmid

   #49424

   Purpose

   Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.

   Depositor

   Daniel Klionsky

   Article

   Abeliovich et al Mol Biol Cell. 2003 Feb;14(2):477-90.

   Insert

   autophagy-related 8 (ATG8 Budding Yeast)
   

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Promoter

   ATG8

   Available Since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pRN110

   Plasmid

   #127228

   Purpose

   CmYLCV::STM::35S terminator

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   STM (STM Mustard Weed, A. tumefaciens)
   

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTpal-fdc

   Plasmid

   #78288

   Purpose

   Expresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coli

   Depositor

   David Nielsen

   Article

   McKenna et al Biotechnol J. 2013 Dec;8(12):1465-75. do

   Inserts

   PAL2 (PAL2 Mustard Weed)
   FDC1 (FDC1 Budding Yeast)
   

   Expression

   Bacterial

   Mutation

   *see below

   Promoter

   trc

   Available Since

   June 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYFP-NGAT

   Plasmid

   #11383

   Depositor

   Joel Swanson

   Article

   Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

   Insert

   golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   March 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRetroSuper-shSrc

   Plasmid

   #26982

   Depositor

   Joan Massague

   Article

   Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

   Insert

   shRNA Src (SRC Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pLenti-CMV-NGLY1-Puro

   Plasmid

   #181916

   Purpose

   Lentiviral plasmid that directs the expression of NGLY1 in mammalian cells.

   Depositor

   Scott Dixon

   Article

   Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

   Insert

   N-glycanase 1 (NGLY1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p8103 LentiCRISPR v2 sgNT-2

   Plasmid

   #163313

   Purpose

   Expresses Cas9 and a non-targeting control guide RNA

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

   Insert

   sgNT-2
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEG302 22aa SunTag NtDRMcd gRNA4 (FWA)

   Plasmid

   #115486

   Purpose

   CRISPR-Cas9 SunTag system to target NtDRMcd to the FWA locus with a single guide RNA

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. NK73

   Plasmid

   #176609

   Purpose

   To test bead engulfment of macrophages.

   Depositor

   Meghan Morrissey

   Article

   Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

   Insert

   SNAP-CD86-FCERG (CD86 Human, Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   n/a

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVenus-N-SLC35B2

    Plasmid

    #155038

    Purpose

    Transient expression of SLC35B2-Venus

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 (SLC35B2 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    CMV

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGWB521

    Plasmid

    #74863

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJMP1333

    Plasmid

    #119268

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. JDW 1050 (pME_V5_GATA1_P2A_H2A_mCherry)

    Plasmid

    #232127

    Purpose

    A middle entry gateway clone containing V5 tagged human GATA1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    GATA1 (GATA1 Human)
    

    Use

    Gateway cloning

    Tags

    V5

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3_2FLAG-AKAP79ΔCN

    Plasmid

    #183451

    Purpose

    Expression of human AKAP79 lacking the calcineurin binding site (Δ337-343)

    Depositor

    Matthew Gold

    Article

    Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

    Insert

    AKAP5 (AKAP5 Human)
    

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    aa337-343 deleted

    Promoter

    CMV

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS-Flag-hsDicer (D1709A)

    Plasmid

    #41588

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D1709A

    Promoter

    CMV

    Available Since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

16. shLacZ

    Plasmid

    #42559

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shLacZ
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

17. Pzac2.1 U6-Fermt2 sgRNA1, 2, 3_gfaABC1D mcherry SV40

    Plasmid

    #179117

    Purpose

    Expresses Fermt2 sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

    Depositor

    Baljit Khakh

    Article

    Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

    Insert

    Fermt2 sgRNA
    

    Use

    AAV

    Promoter

    U6

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHLP700

    Plasmid

    #203742

    Purpose

    C-terminal tagging of target proteins with AID*/3xHA degron

    Depositor

    Mark Hall

    Article

    Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

    Insert

    IAA17 auxin binding domain codons 71-114
    

    Use

    Pcr amplification template for gene tagging

    Tags

    3xHA

    Promoter

    none

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHLP701

    Plasmid

    #203743

    Purpose

    C-terminal tagging of target proteins with 3xV5-IAA17 degron

    Depositor

    Mark Hall

    Article

    Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

    Insert

    IAA17 auxin binding domain
    

    Use

    Pcr amplification template for gene tagging

    Tags

    3xV5

    Promoter

    none

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YFP-Cdc42(N17)

    Plasmid

    #11400

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    cell division cycle 42 (CDC42 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Threonine 17 changed to Asparagine.

    Available Since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMSCV-WT-mAbl

    Plasmid

    #69027

    Purpose

    Retroviral vector encoding WT mouse c-Abl

    Depositor

    Jean Wang

    Article

    Sridevi et al PLoS One. 2013 Oct 11;8(10):e77495. doi:

    Insert

    mouse Abl-Type IV (wild type)
    

    Use

    Retroviral

    Tags

    FLAG tag inserted at Internal SalI site (2934 bp)

    Expression

    Mammalian

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pET-mBoNT/F

    Plasmid

    #168015

    Purpose

    Expresses MBP-BoNT/F-LC(1-428)-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    MBP-BoNT/F-LC(1-428)-6xHis
    

    Tags

    Hisx6 and maltose binding protein

    Expression

    Bacterial

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGWB520

    Plasmid

    #74862

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGWB410

    Plasmid

    #74804

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRS1422

    Plasmid

    #12548

    Depositor

    Robert Sobol

    Article

    Trivedi et al Cancer Res. 2005 Jul 15. 65(14):6394-400

    Insert

    human Methylpurine DNA glycosylase (MPG Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. k3(long) luciferase

    Plasmid

    #11110

    Depositor

    Anjana Rao

    Article

    Tsai et al Mol Cell Biol. 1996 Feb . 16(2):459-67.

    Insert

    k3(L) (TNF Human)
    

    Tags

    luciferase

    Expression

    Bacterial

    Available Since

    April 24, 2006

    Availability

    Academic Institutions and Nonprofits only

27. pHis2-Arf1 (17-181)-Q71L

    Plasmid

    #184808

    Purpose

    Bacterial expression of Arf1 (aa17-181, Q71L mutation)

    Depositor

    James Hurley

    Article

    Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

    Insert

    Arf1 (ARF1 Human)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    aa 17-181 only, Q71L

    Promoter

    T7

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p7274 MSCV-P C-FlagHA 16 ∆DLYC

    Plasmid

    #163304

    Purpose

    Expresses HPV16 E7 ∆DLYC

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

    Insert

    HPV16 E7 ∆DLYC
    

    Use

    Retroviral

    Tags

    FlagHA

    Mutation

    ∆DLYC

    Promoter

    MSCV LTR

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA5/FRT/TO HSPA1L

    Plasmid

    #19457

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPA1L (HSPA1L Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDH-hSyn-NCreIntN-T2A-mTagBFP2

    Plasmid

    #160502

    Purpose

    Expresses NCreIntN in neurons in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    NCreIntN
    

    Use

    Cre/Lox and Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. YFP-mGCN5-C1

    Plasmid

    #134573

    Purpose

    Expresses YFP-GCN5 fusion protein

    Depositor

    Susan Janicki

    Article

    Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

    Insert

    GCN5 (Kat2a Mouse)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 3413 pBF339 Bcl-XL

    Plasmid

    #8773

    Depositor

    Stanley Korsmeyer

    Article

    Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

    Insert

    Bcl-XL (Bcl2l1 Mouse)
    

    Expression

    Yeast

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

33. EPB104

    Plasmid

    #68369

    Purpose

    contains human U6 promoter

    Depositor

    Eric-Paul Bennett

    Article

    Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

    Insert

    U6 promoter (RNU6-1 Human)
    

    Expression

    Bacterial

    Promoter

    U6

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mRIP3 GFP RHIM mut

    Plasmid

    #41384

    Depositor

    Francis Chan

    Article

    Cho et al Cell. 2009 Jun 12;137(6):1112-23.

    Insert

    RIP3 (Ripk3 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    RHIM mutant

    Promoter

    CMV

    Available Since

    Dec. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pIW447-CRISPR XYL2

    Plasmid

    #98906

    Purpose

    K. marxianus CRISPR Plasmid for XYL2 editing

    Depositor

    Ian Wheeldon

    Article

    Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

    Inserts

    Codon optimized Cas9
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-CMP-PE-V1

    Plasmid

    #202769

    Purpose

    Expresses CMP-PE-V1 in mammalian cells for prime editing

    Depositor

    Kyoungmi Kim

    Article

    Park et al Genome Biol. 2021 Jun 3;22(1):170. doi:

    Insert

    CMP-PE-V1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCRISPRai

    Plasmid

    #189938

    Purpose

    Expresses CRISPRai cassette under EF1a promoter

    Depositor

    Yu-Chen Hu

    Article

    Truong et al Nucleic Acids Res. 2019 Jul 26;47(13):e7

    Insert

    CRISPRai cassette
    

    Expression

    Mammalian

    Promoter

    CMV enhancer-rat EF1 alpha

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGWB420

    Plasmid

    #74814

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Tom20-mCherry-GAI(1-92)

    Plasmid

    #37316

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Tags

    Tom20 and mCherry

    Expression

    Mammalian

    Mutation

    contains aa 1-92

    Promoter

    CMV

    Available Since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

40. MSCV eIF4E S209D IRES GFP

    Plasmid

    #18765

    Depositor

    Scott Lowe

    Article

    Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

    Insert

    eIF4E S209D (Eif4e Mouse)
    

    Use

    Retroviral

    Tags

    IRES GFP

    Expression

    Mammalian

    Mutation

    S209D

    Available Since

    July 27, 2008

    Availability

    Academic Institutions and Nonprofits only