We narrowed to 23,273 results for: Sis

Showing: 10601 - 10640 of 23273 results

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pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)

Plasmid

#73404

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB413

Plasmid

#74807

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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linker-mCitrine-PIF6-NAT

Plasmid

#51576

Purpose

Tag protein of interest with PIF6 in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PIF6 (PIL2 Mustard Weed)

Tags

linker and mCitrine

Expression

Yeast

Mutation

1-100aa of PIF6

Promoter

none

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pDGE651

Plasmid

#153229

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB407

Plasmid

#74801

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pET-Galectin-8 FL

Plasmid

#68805

Purpose

Express Galectin-8 FL protein in Bacterial cells

Depositor

Hyun Kyu Song

Article

Kim et al Nat Commun. 2013;4:1613. doi: 10.1038/nc

Insert

Galectin-8 (LGALS8 Human)

Tags

N-terminal 6-histidine-tag

Expression

Bacterial

Mutation

No mutation

Promoter

T7

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pGWB550

Plasmid

#74880

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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Level 1 P1 OsActinP:Hygint:NosT

Plasmid

#165423

Purpose

GoldenGate (MoClo) Level 1 Position 1 (Reverse) hygromycin selection cassette

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Article

Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

Insert

Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator

Use

Synthetic Biology; L1 p1(r) golden gate (moclo) h…

Promoter

Os Actin promoter :: Hygromycin resistance gene (…

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pENO1-BFP-NAT

Plasmid

#50046

Purpose

Expression of BFP in Candida albicans

Depositor

Hidde Ploegh

Article

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

BFP

Expression

Yeast

Promoter

pENO1

Available Since

Jan. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pZ_P-GAP-eGFP

Plasmid

#126717

Purpose

yeast plasmid overexpressing eGFP

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

egfp

Expression

Yeast

Promoter

GAP

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal TDP43 WT

Plasmid

#27458

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Expression

Yeast

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pFloat.T7_v1

Plasmid

#133070

Purpose

Entry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Entry vector

Tags

3C-His6 and His6-3C

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416Gal TDP43 WT YFP

Plasmid

#27447

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Yeast

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pGL3-24p3/LCN2-Luc

Plasmid

#25463

Depositor

Sarah Gaffen

Article

Shen et al J Leukoc Biol. 2005 Mar . 77(3):388-99.

Insert

24p3/lipocalin 2 proximal promoter (Lcn2 Mouse)

Use

Luciferase

Mutation

none

Available Since

June 25, 2010

Availability

Academic Institutions and Nonprofits only
pMRX-IP/SECFP-hATG9A

Plasmid

#58995

Purpose

Expresses SECFP-hATG9A in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

Homo sapiens autophagy related 9A (PPARG Human)

Use

Retroviral

Tags

Venus

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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R26-EGFP HMEJ donor vector

Plasmid

#137927

Purpose

ROSA26 knock-in vector with homology-mediated end joining(HMEJ)-based method

Depositor

Hiroshi Kiyonari

Article

Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

Insert

SA-EGFP-bpA with homology arms

Use

Mouse Targeting

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-RIPK3

Plasmid

#78829

Purpose

retroviral expression of RIPK3

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
TfR-pHoran4

Plasmid

#61547

Purpose

Human transferrin receptor tagged with pH sensitive orange/red fluorescent protein pHoran4

Depositor

David Perrais

Article

Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

Insert

Transferrin receptor (TFRC Human)

Tags

pHoran4

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TK1

Plasmid

#100544

Purpose

mammalian expression of thymidine kinase 1

Depositor

Yuh-Shan Jou

Article

Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

Insert

thymidine kinase 1 (TK1 Human)

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-CAG-IntCCCre-T2A-CopGFP

Plasmid

#160501

Purpose

Expresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

IntCCCre

Use

Cre/Lox and Lentiviral

Tags

CopGFP

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP1183

Plasmid

#119254

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 MYC

Plasmid

#176045

Purpose

Expresses Myc in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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YFP-Cdc42(V12)

Plasmid

#11399

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

cell division cycle 42 (CDC42 Human)

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Glycine 12 changed to Valine.

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

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tdTomato-β-actinCDS3

Plasmid

#183715

Purpose

tdTomato with fragment nucleotides 801-1127 of beta-actin coding sequence inserted in 3'UTR

Depositor

Jeffrey Gerst

Article

Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

Insert

tdTomato with fragment (nucleotides 801-1127) of mouse Actb coding dsequence

Use

Lentiviral

Promoter

UbiC

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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458 p3E-2A-EGFP-CAAXpA

Plasmid

#195968

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-EGFP-CAAX (membrane)

Use

Other

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pT2Apuro-Stargazin-mEGFP-iLID

Plasmid

#178529

Purpose

Expresses Stargazin-mEGFP-iLID in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Stargazin-mEGFP-iLID

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-delta90GFP

Plasmid

#26645

Depositor

Anjen Chenn

Article

Wrobel et al Dev Biol. 2007 Sep 15. 309(2):285-97.

Insert

delta90 beta-catenin (Ctnnb1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of first 90 aa of beta catenin

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

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UKNP2.4.1

Plasmid

#98212

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pJL1-sfGFP-DQNAT

Plasmid

#198210

Purpose

pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

sfGFP_DQNAT

Tags

6x His Tag and DQNAT glycosylation tag

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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NET33 pEGFP-N2 (597)

Plasmid

#61989

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET33 (SCARA5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Nanog-2A-GFP

Plasmid

#59994

Purpose

Inserts GFP immediately downstream of the Nanog coding region

Depositor

Rudolf Jaenisch

Article

Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

Insert

Nanog (Nanog Mouse)

Use

Mouse Targeting

Tags

2A-GFP

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pKD4-Nter-APEX2

Plasmid

#112868

Purpose

Bacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner method

Depositor

Eric Cascales

Article

Santin et al Nat Microbiol. 2018 Nov;3(11):1304-1313.

Insert

APEX2

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MC177VSG221RNAi

Plasmid

#59730

Purpose

For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

Depositor

Gloria Rudenko

Article

Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

Insert

VSG221 RNAi fragment

Use

RNAi

Promoter

opposing T7 promoters

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry

Plasmid

#124493

Purpose

Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipYFP(TEV cleavage seq) T2A mCherry

Use

Synthetic Biology

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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1255_pAAV-U6-SA-BbsI-MluI-gRNA-CB-SACas9-HA-OLLAS-spA

Plasmid

#109320

Purpose

Plasmid for AAV SaCas9 Mammalian Expression with a BbsI cloning site for a gRNA

Depositor

William Lagor

Article

Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFloat.cspA_v1

Plasmid

#133071

Purpose

Entry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Entry vector

Tags

3C-His6 and His6-3C

Expression

Bacterial

Promoter

cspA

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUW-tetO-mCherry

Plasmid

#193060

Purpose

doxycycline-inducible expression of mCherry in mammalian cell

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

minimal CMV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pdCas9-omega

Plasmid

#114632

Purpose

Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

Depositor

Anushree Chatterjee

Article

Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

Insert

dCas9-omega

Use

CRISPR

Expression

Bacterial

Promoter

Tet promoter

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT311_pH11-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52544

Purpose

Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Expression

Mammalian

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
JDW 1043 (pLenti6.2_CMV_V5_LEF1_P2A_H2A_mCherry)

Plasmid

#233538

Purpose

A CMV driven human LEF1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

LEF1 (LEF1 Human)

Use

Lentiviral

Tags

V5

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 23,273 results for: Sis