19,653 results

1. pBABE-zeo largeTcDNA

   Plasmid

   #1779

   Depositor

   Bob Weinberg

   Article

   Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

   Insert

   large T antigen (SV40gp6 simian virus)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-mem-TurboID

   Plasmid

   #149409

   Purpose

   Mammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selection

   Depositor

   Jonathan Long

   Article

   Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

   Insert

   mem-TurboID
   

   Tags

   HA and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-JEDI-2P-WPRE

   Plasmid

   #179464

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Has Service

   AAV9

   Insert

   JEDI-2P
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDule2-pCNF

   Plasmid

   #85495

   Purpose

   Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

   Insert

   para-cyanophenylalanine Mj synthetase
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   lpp (constitutive)

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FLAG-LKB1

   Plasmid

   #8590

   Depositor

   Lewis Cantley

   Article

   Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

   Insert

   LKB1 (STK11 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABEpuro mTurquoise2 LC3B

   Plasmid

   #78518

   Purpose

   Fluorescent reporter for LC3B visualization

   Depositor

   David Chan

   Article

   Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

   Insert

   LC3B (MAP1LC3B Human)
   

   Use

   Retroviral

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   retroviral

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti6-MK1-EHMT2-V5

   Plasmid

   #31113

   Depositor

   Bernard Futscher

   Insert

   euchromatic histone-lysine N-methyltransferase 2 (EHMT2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Stop codon mutated to Ala

   Available Since

   Aug. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV3flag8HOIP

   Plasmid

   #50015

   Purpose

   Mammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection.

   Depositor

   Martin Dorf

   Article

   Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

   Insert

   ring finger protein 31 (RNF31 Human)
   

   Tags

   3 x FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRXIP GFP-Stx7

   Plasmid

   #45921

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

   Insert

   syntaxin 7 (STX7 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Promoter

   CMV

   Available Since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

10. mApple-C1

    Plasmid

    #54631

    Purpose

    Localization: C1 Cloning Vector, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Type

    Empty backbone

    Tags

    mApple

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-SARS-CoV-2-S-RBD-8his

    Plasmid

    #145145

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    

    Tags

    8his tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1hSIRT5_HA

    Plasmid

    #24483

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Sirtuin 5 (SIRT5 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. Halo-Sec61-C-18

    Plasmid

    #123285

    Purpose

    Localization: Endoplasmic Reticulum

    Depositor

    Kevin McGowan

    Insert

    Sec61 (SEC61B Human)
    

    Tags

    HaloTag7

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. SPLICS Po-Mt Long P2A

    Plasmid

    #164115

    Purpose

    Detect the long Peroxisomes-mitochondria contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Po-Mt Long P2A
    

    Expression

    Mammalian

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Salsa6f

    Plasmid

    #140188

    Purpose

    Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)

    Depositor

    Michael Cahalan

    Article

    Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1

    Insert

    Salsa6f
    

    Tags

    tdTomato-v5-GCaMP6f

    Expression

    Mammalian

    Promoter

    CMV Immediate Early

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDF-GFPplus

    Plasmid

    #172718

    Purpose

    Expression of a fast folding GFP variant GFP plus

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    GFP plus
    

    Expression

    Bacterial

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 HA-humanTRAF2 (1084)

    Plasmid

    #66932

    Purpose

    Mammalian Expression of TRAF2

    Depositor

    Michael Karin

    Insert

    TRAF2 (TRAF2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)

    Plasmid

    #49077

    Purpose

    Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Tags

    3x HA and mCerulean

    Expression

    Mammalian

    Mutation

    262 silent mutations from native hNKCC1

    Promoter

    CMV

    Available Since

    Nov. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6a:sgRNA#1

    Plasmid

    #64245

    Purpose

    expresses sgRNA under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. XLone-Puro eGFP

    Plasmid

    #140027

    Purpose

    Tunable and temporal expression control of nuclear eGFP

    Depositor

    Xiaoping Bao

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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