164,577 results

1. pQE30-roGFP1-iR

   Plasmid

   #83314

   Purpose

   Inducible expression of redox sensitive GFP in bacteria

   Depositor

   S. James Remington

   Article

   Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

   Insert

   roGFP1-iR
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   ro1(C48S/S147C/Q204C) plus X insertion, iR betwe…

   Promoter

   T5

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pOpen-MMLV_RT (lack H)

   Plasmid

   #165556

   Purpose

   Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. SPLICS PM-ER Long P2A

   Plasmid

   #164111

   Purpose

   Detect the long Plasma membrane-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP PM-ER Long P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3Luc-5XNF-kappaB

   Plasmid

   #185695

   Purpose

   Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

   Depositor

   Esther López-Bayghen

   Article

   Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

   Insert

   NF-kappaB (NFKB1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   NF-kappaB binding site

   Available Since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-GfaABC1D-lck-smMyc-4x6T-WPRE

   Plasmid

   #196415

   Purpose

   Astrocytic expression of membrane-targeted Myc spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Insert

   Lck-smMyc
   

   Use

   AAV; Astrocyte-selective

   Tags

   Lck

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and GfaABC1D

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. HSA1_His

   Plasmid

   #202763

   Purpose

   human serum albumin design HSA1, in pET29b with C-terminal 6His-tag

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

   Insert

   HSA1
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBR322-PVCpnf17-22

   Plasmid

   #198272

   Purpose

   Unmodified pPayload; contains WT PVC payloads/regulatory genes

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf17-22
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-CMV-PEmax-IRES-ZeoR

   Plasmid

   #207353

   Purpose

   Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   Prime editor max (PEmax) enzyme
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIMAY

   Plasmid

   #68939

   Purpose

   E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange

   Depositor

   Tim Foster

   Article

   Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.

   Type

   Empty backbone

   Use

   Staphylococcal allelic exchange vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL1119 (pDonor)

    Plasmid

    #160732

    Purpose

    Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST donor DNA (Mini-Tn)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

    Plasmid

    #165450

    Purpose

    Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

    Depositor

    Elvir Becirovic

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Inserts

    dCas9N-IntN
    3x Opn1mw promoter-targeting sgRNAs
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and short human rhodopsin promoter

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1-TetON-Puro-HIF1a 0819

    Plasmid

    #118704

    Purpose

    Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    shRNA against human HIF1a
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFD6

    Plasmid

    #73915

    Purpose

    tissue-specific multiplex gRNA plasmid

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    :tRNA:gRNA:tRNA:gRNA:tRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS-DSCP

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB533-42B3mutAC2-sfGFP

    Plasmid

    #186777

    Purpose

    RNAP2 Ser2ph-mintbody [42B3(R78K/A80T/M95V)-scFv] expression vector for mammalian cells

    Depositor

    Hiroshi Kimura

    Article

    Uchino et al J Cell Biol. 2022 Feb 7;221(2). pii: 212

    Insert

    42B3mutAC2-scFv
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV2.5-THP-GFP

    Plasmid

    #80336

    Purpose

    Fluorescent Reporter for Dopaminergic Neuron Differentiation

    Depositor

    Kwang-Soo Kim

    Article

    Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

    Insert

    rat TH promoter (Th Rat)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    rat Tyrosine hydroxylase

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG_smFP V5

    Plasmid

    #59758

    Purpose

    encodes multiple copies of an epitope tag V5

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _V5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. psfaRb-Tn5

    Plasmid

    #183638

    Purpose

    Expresses anti-rabbit nanobody fused with Tn5

    Depositor

    Goncalo Castelo-Branco

    Article

    Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

    Insert

    anti rabbit secondary nanobody fused to Tn5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 HA Arf6

    Plasmid

    #10834

    Depositor

    Thomas Roberts

    Article

    Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Syn-ChrimsonR-GFP

    Plasmid

    #58806

    Purpose

    AAV expression of ChrimsonR-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K176R

    Promoter

    Syn

    Available Since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV-opEBNA2-IRES-GFP

    Plasmid

    #220354

    Purpose

    Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Tags

    3 x HA tag

    Expression

    Mammalian

    Mutation

    codon-optimised for expression in mammalian cells

    Promoter

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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