We narrowed to 29,763 results for: REP

1. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

   Plasmid

   #64216

   Purpose

   Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA targeting ROSA26-1
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-BFP

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-UBC-S2-cAMPr

   Plasmid

   #160723

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter cAMPr (cAMP indicator), HA tag, and S2 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S2-cAMPr
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. mARGAna (cassette 2, transient)

   Plasmid

   #197589

   Purpose

   Second-generation mammalian acoustic reporter gene derived from Anabaena

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   mARGAna (cassette 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro

   Plasmid

   #89991

   Purpose

   Donor template for generation of SOX2-ntdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

   Insert

   SOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   none

   Available Since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRE3G-PE2-P2A-BFP

   Plasmid

   #231580

   Purpose

   Expresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA protein

   Depositor

   Jesse Engreitz

   Article

   Martyn et al bioRxiv [Preprint]. 2023 Dec 21:2023.12.

   Insert

   PE2-P2A-BFP
   

   Use

   Lentiviral

   Tags

   P2A-BFP

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

   Plasmid

   #207805

   Purpose

   E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.

   Depositor

   Koen Breyne

   Article

   Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do

   Insert

   E-NoMi
   

   Use

   Lentiviral

   Tags

   3xFlag tag, copGFP, mCherry, and nanoluciferase

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNIC-ZB-SPRTNwt-FL

   Plasmid

   #110217

   Purpose

   Expression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.

   Depositor

   Kristijan Ramadan

   Article

   Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

   Insert

   SPRTN (SPRTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   P296L (see depositor comments below)

   Promoter

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC475 - pAAV c-fos iRFP

   Plasmid

   #47906

   Purpose

   An AAV vector that expresses iRFP under the c-fos promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   Infrared fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   c-fos

   Available Since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-UBC-S1-GCaMP6f

   Plasmid

   #160722

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S1-GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOTTC589 - pAAV c-fos Nuc-eYFP

    Plasmid

    #59133

    Purpose

    An AAV vector that expresses nuclear localized eYFP under control of the c-fos promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    Nuclear-localized Enhanced Yellow Fluorescent Protein
    

    Use

    AAV

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    mFos

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLL3.7-CAG-hMGT-Ef1a-puro

    Plasmid

    #172395

    Purpose

    Lentiviral expression vector for human direct cardiac reprogramming

    Depositor

    Li Qian

    Article

    Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

    Insert

    Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLL3.7-UBCp-hMGT-Ef1a-puro

    Plasmid

    #172396

    Purpose

    Lentiviral expression vector for human direct cardiac reprogramming

    Depositor

    Li Qian

    Article

    Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

    Insert

    Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOTTC337 - pAAV EF1a DIO mCherry

    Plasmid

    #47636

    Purpose

    An AAV vector that expresses mCherry in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    mCherry
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOTTC364 - pAAV CaMKIIa iRFP

    Plasmid

    #47903

    Purpose

    An AAV vector that expresses iRFP under the CaM Kinase IIa promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    Infrared fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOTTC588 - pAAV c-fos Nuc-iRFP

    Plasmid

    #59132

    Purpose

    An AAV vector that expresses nuclear localized iRFP under control of the c-fos promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    Nuclear-localized Infrared Fluorescent Protein
    

    Use

    AAV

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    mFos

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-UBC-S3-ExRaiAKAR

    Plasmid

    #160724

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S3-ExRaiAKAR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-UBC-S4-ExRaiCKAR

    Plasmid

    #160725

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S4-ExRaiCKAR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pmx-puro-MiGT

    Plasmid

    #172399

    Purpose

    Retroviral expression vector for murine direct cardiac reprogramming

    Depositor

    Li Qian

    Article

    Wang et al Cells. 2020 Jan 22;9(2). pii: cells90202

    Insert

    Mef2c-i, Gata4, Tbx5 (Gata4, Tbx5, Mef2c Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-UBC-S2-RAB_EKARev

    Plasmid

    #160726

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S2-RAB_EKARev
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-UBC-S3-RAB_EKARev

    Plasmid

    #160727

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S3-RAB_EKARev
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-UBC-S1-jRCaMP1a

    Plasmid

    #160728

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S1-jRCaMP1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLV.tetO.Nurr1

    Plasmid

    #234848

    Purpose

    3rd generation lentiviral vector, expresses Nurr1 under control of TetON promoter

    Depositor

    Malin Parmar

    Article

    Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

    Insert

    Nurr1 (Nr4a2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFB1.HMBP.PrS.CBX8ΔChromo

    Plasmid

    #224708

    Purpose

    Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 chromodomain truncation (CBX8 Human)
    

    Use

    Tags

    HMBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFBOH-MHL.mEGFP-CBX8

    Plasmid

    #224710

    Purpose

    Expresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 (CBX8 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    Oct. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pFB1.HMBP.PrS.CBX8-KR21A

    Plasmid

    #224709

    Purpose

    Expresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 KR21A mt (CBX8 Human)
    

    Use

    Tags

    HMBP

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFB1.HMBP.PrS.CBX8

    Plasmid

    #224707

    Purpose

    Expresses human CBX8 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 (CBX8 Human)
    

    Use

    Tags

    HMBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SS-Cyclin D3

    Plasmid

    #215116

    Purpose

    Expresses SS-Cyclin D3 in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Tags

    SS (STREP-STREP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLV.tetO.Ascl1.U6.shRest1.U6.shRest2

    Plasmid

    #234846

    Purpose

    3rd generation lentiviral vector, expresses Ascl1 under control of TetON promoter and two shREST sequences under control of U6 promoters

    Depositor

    Malin Parmar

    Article

    Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p2T-CMV-EA-evoAPOBEC1max-32NLS_NG-BlastR

    Plasmid

    #232722

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

    Insert

    evoAPOBEC1max H47E+S48A-32NLS linker-nCas9(NG)-UGI-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    within evoAPOBEC1: H47E+S48A; within Cas9 D10A

    Promoter

    Available Since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV-TetO-hNGN2-Neo

    Plasmid

    #99378

    Purpose

    Expresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    hNGN2-P2A-Neo (NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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