Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 156,064 results for: ins

Clear keywords
Showing: 8011 - 8040 of 156064 results

  2. AAVS1-iSAM

    Plasmid
    #211495
    Purpose
    AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa
    Depositor
    Antonella Fidanza, Lesley Forrester
    Article
    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7
    Inserts
    UniSAM insert
    TET-ON
    Neo Resistance
    Insulator
    Use
    CRISPR and Synthetic Biology
    Tags
    T2A-mCherry Tag
    Expression
    Mammalian
    Promoter
    EF1a, NA, Splicing acceptor, and TREGV promoter
    Available Since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    211495-plasmid-map-sequence-id-454077

    Enlarge

  3. pB80-hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro)

    Plasmid
    #193721
    Purpose
    Optogenetic coupling to dimeric human kinesin-1 (KIF5B aa1-807) via SSPB(micro) for anterograde transport
    Depositor
    Lukas Kapitein
    Article
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Insert
    hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro) (KIF5B Synthetic, Human)
    Tags
    mVenus-SSPB(micro)
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …
    Promoter
    Chicken beta-actin
    Available Since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193721-plasmid-map-sequence-id-383728

    Enlarge

  4. G protein alpha-q-GFP

    Plasmid
    #66080
    Purpose
    This G protein alpha-q construct contains internal insertions of GFP and the EE epitope
    Depositor
    Catherine Berlot
    Article
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Insert
    G protein alpha-q-EE-YFP (Gnaq Mouse)
    Tags
    GFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available Since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    66080-plasmid-map-sequence-id-436984

    Enlarge

  6. TDP-REGv2(mScarlet-FLAG)#11 in pTwist-CMV backbone

    Plasmid
    #216155
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Very bright and decent dynamic range (>10-fold), slightly leaky. It uses a single intron. [Code 'B12']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216155-plasmid-map-sequence-id-437763

    Enlarge

  11. AAV-pCBh-ABE8e-nSpCas9(D10A)[N-term] (HES1468)

    Plasmid
    #197509
    Purpose
    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A)
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A)[N-term]-NpuN-BPNLS-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    ABE8e mutations in TadA and nSpCas9(D10A)
    Promoter
    Cbh
    Available Since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197509-plasmid-map-sequence-id-391242

    Enlarge

  12. His-ZZ-TEV-SNAPf-DYNC2H1

    Plasmid
    #132524
    Purpose
    Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragments
    Depositor
    Andrew Carter
    Article
    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1
    Insert
    DYNC2H1 (DYNC2H1 Human)
    Tags
    8x His, Linker-TEV-linker-TEV, Precission cleavag…
    Expression
    Insect
    Mutation
    Codon optimised for Sf9 expression
    Available Since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    132524-plasmid-map-sequence-id-261476

    Enlarge

  14. pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

    Plasmid
    #185916
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8.20m-nSpG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185916-plasmid-map-sequence-id-367354

    Enlarge

  16. pCMVtrunc msfGFPΔC9-LifeactΔN2

    Plasmid
    #231556
    Purpose
    Mammalian expression of N-terminally truncated Lifeact fused to C-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy
    Depositor
    Manos Mavrakis
    Article
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    Lifeact (ABP140 Budding Yeast)
    Tags
    msfGFP
    Expression
    Mammalian
    Promoter
    CMVtrunc
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231556-plasmid-map-sequence-id-459689

    Enlarge

  24. lucMAPT-30D

    Plasmid
    #214672
    Purpose
    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs downstream of the 5′ splice site
    Depositor
    Eugene Yeo
    Article
    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    MAPT (MAPT Human)
    Tags
    Firefly Luciferase, Renilla Luciferase, and V5
    Expression
    Mammalian
    Mutation
    Contains Exon 9, Exon 10 with a mutation to intro…
    Promoter
    CMV
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214672-plasmid-map-sequence-id-424908

    Enlarge

  25. lucMAPT-30U

    Plasmid
    #214673
    Purpose
    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs upstream of the 3′ splice site
    Depositor
    Eugene Yeo
    Article
    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    MAPT (MAPT Human)
    Tags
    Firefly Luciferase, Renilla Luciferase, and V5
    Expression
    Mammalian
    Mutation
    Contains Exon 9, Exon 10 with a mutation to intro…
    Promoter
    CMV
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214673-plasmid-map-sequence-id-424933

    Enlarge

  28. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)

    Plasmid
    #208959
    Purpose
    A variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208959-plasmid-map-sequence-id-415785

    Enlarge

Showing: 8011 - 8040 of 156064 results