We narrowed to 10,893 results for: ENA;

1. MAC_N_CDK7

   Plasmid

   #111684

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   CDK7 (CDK7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSC4

   Plasmid

   #91182

   Purpose

   T-DNA vector for targeted deletion of 58kb region in Medicago truncatula (tRNA array of 6 gRNAs under the control of CmYLCV promoter)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   gRNAs targeting 58kb region in medicago truncatula
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pegAAT K342E correction

   Plasmid

   #169841

   Purpose

   Express a pegRNA used for correction (via A•T-to-G•C) of the E342K mutation

   Depositor

   Wen Xue

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   pegAAT K342E correction (SERPINA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. sTR056

   Plasmid

   #177369

   Purpose

   Toehold switch, with an unpaired toehold region at the 5′-end that interacts with the trigger RNA (sTR060, Addgene plasmid # 177370) to unfold the switch.

   Depositor

   Jean-Loup Faulon, Jerome Bonnet

   Article

   Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. sTR060

   Plasmid

   #177370

   Purpose

   Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

   Depositor

   Jean-Loup Faulon, Jerome Bonnet

   Article

   Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

   Insert

   Trigger RNA
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. His-ZZ-TEV-BICDR1

   Plasmid

   #111859

   Purpose

   Full length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ.

   Depositor

   Andrew Carter

   Article

   Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

   Insert

   BICDR1 (Bicdl1 Mouse)
   

   Use

   Tags

   His-tag, TEV site, and ZZ

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRIP-SFFV-GFP-RELA K5R

   Plasmid

   #196631

   Purpose

   Express GFP-RELA K5R

   Depositor

   Nicolas Manel

   Article

   Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

   Insert

   GFP-RELA K5R (RELA Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   K122R, K123R, K310R, K314R, K315R

   Promoter

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBC2T-Ypet-FtnA

   Plasmid

   #108969

   Purpose

   expresses BC2-tag-Ypet-FtnA in mammalian cells

   Depositor

   Ulrich Rothbauer

   Article

   Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

   Insert

   FtnA (ftnA Escherichia coli)
   

   Use

   Tags

   BC2-tag and Ypet

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPKm-245

   Plasmid

   #90503

   Purpose

   Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - P2A - MTS - tFD - P2A - MTS - tFNR

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPKm-244

    Plasmid

    #90502

    Purpose

    Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - IRES - MTS - tFD - P2A - MTS - tFNR

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. 8xCTS1_WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

    Plasmid

    #124541

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    WT-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_WT-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT, WT

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEntr1a-mcherry-FKBP-TIAM1(DHPH)

    Plasmid

    #176135

    Purpose

    Heterodimerization with FRB (rapamycin), Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    See Depositor Comments Below

    Promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMOD_C2516b

    Plasmid

    #91085

    Purpose

    Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    At7SL

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-HA-VprBP (RARA)

    Plasmid

    #133587

    Purpose

    Expresses human VprBP RARA mutant in mammalian cells

    Depositor

    Yue Xiong

    Article

    Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

    Insert

    Vpr (HIV-1) binding protein (DCAF1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    RARA mutation of human VprBP defective in DDB1 bi…

    Promoter

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGW05 AAV-CRISPRa base vector pAAV-OE_U6-Lib(MS2)-EF1a-MPH-sPA

    Plasmid

    #192159

    Purpose

    AAV-CRISPRa base vector

    Depositor

    Sidi Chen

    Article

    Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGY416

    Plasmid

    #166660

    Purpose

    For light-inducible Cre recombinase (LiCre) expression under the Gal1 promoter in yeast

    Depositor

    Gaël Yvert

    Article

    Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

    Insert

    LiCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Cre: E340A, D341A

    Promoter

    GAL1

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmCherry-VIM-BC2T

    Plasmid

    #108966

    Purpose

    expresses mCherry-vimentin-BC2-tag in mammalian cells

    Depositor

    Ulrich Rothbauer

    Article

    Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

    Insert

    VIM (VIM Human)
    

    Use

    Tags

    BC2T and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPKm-112

    Plasmid

    #90494

    Purpose

    pcDNA3 - pCMV - MTAD - PIF3, expresses Phytochrome interacting factor 3 (PIF3) and minimal transactivation domain (MTAD), under CMV promoter

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    MTAD-PIF3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124539

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55A, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-CS-154

    Plasmid

    #166230

    Purpose

    lenti-U6-gRNA::CMVp-nCas9-PmCDA1-UGI-2A-mCherry

    Depositor

    Nozomu Yachie

    Article

    Fukushima et al Sci Rep. 2021 Dec 13;11(1):23889. doi: 1

    Inserts

    U6-gRNA EGFP targetting
    nCas9-PmCDA1-UGI-2A-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV promoter and Human U6

    Available Since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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