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pDNA5-FRT/TO-eGFP-TONSL

Plasmid

#221012

Purpose

mammalian expression vector of eGFP tagged TONSL siRNA resistant

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

TONSL (TONSL Human)

Tags

eGFP

Expression

Mammalian

Mutation

silent mutations in TONSL: 5’-gaATtAgaTCtaTCGatga…

Promoter

CMV

Available Since

June 24, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-G88P3-HA-hM3Dq

Plasmid

#213972

Purpose

AAV vector with G88P3 promoter that can drive robust gene expression in MSNs

Depositor

Zhonghua Lu

Article

Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

Insert

hM3D (Gq)

Use

AAV

Promoter

G88P3

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_CCK1.0

Plasmid

#208673

Purpose

Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0

Use

AAV

Promoter

hSyn

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_NPYmut

Plasmid

#208678

Purpose

Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in mammalian cells

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-EGFP-CAAX

Plasmid

#208684

Purpose

Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

membrane-localized enhanced green fluorescent protein EGFP-CAAX

Use

AAV

Promoter

hSyn

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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1D3 HC

Plasmid

#170670

Purpose

Mammalian Expression Plasmid of Anti-cytochrome c, 1D3 HC (Human)

Depositor

Ronald Jemmerson, Rafel Radi

Article

Tomasina et al Proc Natl Acad Sci U S A. 2022 Nov 22;11

Insert

HC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody

Expression

Mammalian

Available Since

July 12, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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1D3 LC

Plasmid

#170669

Purpose

Mammalian Expression Plasmid of Anti-cytochrome c, 1D3 LC (Human)

Depositor

Ronald Jemmerson, Rafel Radi

Article

Tomasina et al Proc Natl Acad Sci U S A. 2022 Nov 22;11

Insert

LC of anti-cytochrome c, 1D3 (human) recombinant mouse monoclonal antibody

Expression

Mammalian

Available Since

June 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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hPER2.2-luc

Plasmid

#250937

Purpose

Lentiviral vector encoding a truncated promoter region of human PER2 gene driving a luciferase reporter

Depositor

Michelle Farkas

Article

Kalyanaraman et al Int J Mol Sci. 2025 Nov 6;26(21):10785.

Insert

PERIOD 2 Promoter (PER2 Human)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

contains 952 bp of the truncated human PER2 promo…

Promoter

PER2

Available Since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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pCB-CBEv2

Plasmid

#221139

Purpose

Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial

Promoter

lacIq-Ptac

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNES-EGFP-C1-flex-PABD-Spo20

Plasmid

#220081

Purpose

Spo20-PABD PA biosensor with a flexible linker to aid in nuclear export

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

Spo20 (SPO20 Budding Yeast)

Tags

X. leavis map2k1.L(32-44)-EGFP-SGGGSGGS

Expression

Mammalian

Mutation

amino acids 51-91

Promoter

CMV

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pIA033

Plasmid

#200420

Purpose

thrC::(bsPamCherry1 erm2), for bsPAmCherry1 fusions. PALM integration vector includes codon optimized PAmCherry1 FP for B. subtilis. MCS for fast and interchangeable cloning.

Depositor

Rut Carballido-López

Article

Altinoglu et al Microbiol Spectr. 2024 Nov 4;12(12):e016

Type

Empty backbone

Tags

bsPAmCherry

Expression

Bacterial

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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LLP788_Traptavidin-EZH2-FL-CO

Plasmid

#211781

Purpose

AviTag counterpart binding domain, Traptavidin, fused to polycomb repressive complex subunit EZH2, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-EZH2

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect EZH2 e…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT RAD23A

Plasmid

#201458

Purpose

Expresses human RAD23A with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT RAD23B

Plasmid

#201459

Purpose

Expresses human RAD23B with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23B

Plasmid

#201439

Purpose

Expresses human RAD23B with an N-terminal FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.1 HA pCAGGS

Plasmid

#206076

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRRP12_K

Plasmid

#146766

Purpose

Mammalian Expression of HsRRP12

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsRRP12 (RRP12 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-neo-μ1A-(HA)3

Plasmid

#198177

Purpose

Expression of HA-tagged AP-1 μ1A in mammalian cells

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-1 μ1A

Tags

triple HA tag

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pRevTRE-hygro-GFP-Crumbs3a WT

Plasmid

#195217

Purpose

Retroviral Tet response vector containing GFP tagged Crumbs3a

Depositor

David Bryant, Ben Margolis

Article

Schluter et al Mol Biol Cell. 2009 Nov;20(22):4652-63.

Insert

CRB3A (CRB3 Human)

Use

Retroviral

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCK341

Plasmid

#192640

Purpose

Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Inserts

Sp.pCas9-dSpRY
BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR and Synthetic Biology

Mutation

SoxS has R93A and S101A mutations and dSpRY has s…

Promoter

BBa_J23107 and Sp.pCas9

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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