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Plasmid#209519PurposeExpresses luciferase under control of Ptgs2 promoter in transfected cellsDepositorInsertPtgs2 (Ptgs2 Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
XLone-Cas9-2A-GFP
Plasmid#170159PurposeExpress Cas9 and GFP upon doxycycline treatmentDepositorInsertCas9
UseSynthetic Biology; PiggybacTagsGFPExpressionMammalianMutationPromoterTRE3G promoterAvailable sinceJuly 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
XLone-ETV2
Plasmid#135763Purposeexpresses human ETV2 upon doxycycline treatmentDepositorInsertETV2 (ETV2 Human)
UseTagsExpressionMammalianMutationnonePromoterTRE3GAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAG-SaCas9-WPRE
Plasmid#89996PurposeExpresses high levels of WT SaCas9 for genome editingDepositorInsertSaCas9 (NEWENTRY Staphylococcus aureus)
UseTags3xHA, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterCAGAvailable sinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-sh-mSlug-4
Plasmid#40648DepositorInsertsh-mSlug-4 (Snai2 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-sh-mSlug-3
Plasmid#40647DepositorInsertsh-mSlug-3 (Snai2 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCIG-V5-Snail2-IRES-nls-GFP
Plasmid#44282DepositorInsertV5-Snail2 (SNAI2 Chicken)
UseTagsV5ExpressionMammalianMutationPromoterCMV-IE-enhancer and chicken beta-actin promoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
FOR human NANOS3 TALEN
Plasmid#59722PurposeTALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)DepositorInsertHD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG
UseTALENTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
REV human NANOS3 TALEN
Plasmid#59723PurposeTALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)DepositorInsertNG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI
UseTALENTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
SEPTOPO
Plasmid#64944Purposesource of SEP (Super Ecliptic pHluorin) sequenceDepositorInsertSEP
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Human T (BRACHYURY) sgRNA
Plasmid#59726PurposePlasmid encoding sgRNA to generate T (BRACHYURY) knock-out mutant human cellsDepositorInserthT sgRNA (TBXT Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMRMTK-clo13
Plasmid#203983PurposeCloning plasmid containing the tonB & SoxR terminators insert with a chloramphenicol resistance markerDepositorInserttonB & SoxR Terminator
UseTagsExpressionPlantMutationPromoterN/AAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLUT7 HA-GLI1
Plasmid#62970PurposeExpresses HA-tagged Gli1DepositorInsertGLI1 (GLI1 Human)
UseLentiviralTagsHAExpressionMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pBABEPuro-HA-GLI1
Plasmid#62967PurposeExpresses HA-tagged Gli1DepositorInsertGLI1 (GLI1 Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10713
Plasmid#183958PurposeU6-crSox2-CAG-hyperdCas12a-miniVPRDepositorInsertsHyperdCas12a
crRNA targeting Sox2
UseCRISPRTagsHAExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCAG and U6Available sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Human NANOS3-P2A-mCherry knock-in targeting construct
Plasmid#59721PurposeGene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.DepositorInsertP2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-hCas9-T2A-mStrawberry
Plasmid#187224PurposeExpresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.DepositorInsertCas9
UseCRISPRTagsInserted T2A-mStrawberry at 3' terminal of M…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG eGFP-Oct4
Plasmid#203789PurposeCompetitive assay of Oct4 and Oct6 binding to DNA motifs.DepositorInsertPou5f1 (Pou5f1 Mouse)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-Hes1
Plasmid#36983DepositorInsertHes1 (Hes1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only