We narrowed to 17,394 results for: STO

1. pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP

   Plasmid

   #102594

   Purpose

   Lentivector to express STING V155M delta 342 and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   V155M delta 342

   Promoter

   SFFV

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p663-UBC-2xV5-miniTurbo-NES-STOP_IDG-K

   Plasmid

   #158161

   Purpose

   Gateway destination clone of miniTurbo-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   2xV5-miniTurbo-NES
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. iD20 LoxStopLox-Zim3-dCas9-mApple-hygro

   Plasmid

   #204720

   Purpose

   Cre-inducible CRISPRi by Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2

   Plasmid

   #127360

   Purpose

   gateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   BirA* (R118G mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NLS, V5, and YFP

   Expression

   Mutation

   R118G

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. C514-E47: attB5r-Halotag-nostop-attB1r

   Plasmid

   #162963

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Halotag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2

   Plasmid

   #127357

   Purpose

   gateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   BirA* (R118G mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and YFP

   Expression

   Mutation

   R118G

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTRIP-SFFV-mtagBFP-2A STING V155M 354STOP

   Plasmid

   #102595

   Purpose

   Lentivector to express STING V155M delta 354 and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   V155M delta 354

   Promoter

   SFFV

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. C511-E02: attB5r-NanoLuc-nostop-attB1r

   Plasmid

   #162938

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Nanoluciferase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. C514-E44: attB5r-SNAP-nostop-attB1r

   Plasmid

   #162962

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal SNAP-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   SNAP tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. C512-E23: attB5r-mRuby3-nostop-attB1r

    Plasmid

    #162945

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal mRuby3 fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    monomeric Ruby3 fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. C512-E22: attB5r-mClover3-nostop-attB1r

    Plasmid

    #162944

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal mClover3 fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    monomeric Clover3 fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycPC

    Plasmid

    #71812

    Purpose

    Generating transgenic flies for FLP-inducible DamID

    Depositor

    Bas van Steensel

    Article

    Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

    Insert

    min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-PC
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. YFP(159-238)Stop in pcDNA1/amp

    Plasmid

    #60891

    Purpose

    This vector attaches YFP(159-238) to the C-terminus of a protein.

    Depositor

    Catherine Berlot

    Insert

    YFP(159-238)Stop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. C512-E04: attB5r-mCherry-nostop-attB1r

    Plasmid

    #162941

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal mCherry fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    monomeric Cherry fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p252 pPr-CREM/CMV-STOP-luc

    Plasmid

    #8406

    Purpose

    Expression of modified Cre from androgen-inducible probasin promoter and LOX-dependent conditional reporter cassette.

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    CREM/lox/luciferase
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    modified Cre

    Promoter

    Probasin promoter, CMV

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

16. (829) SB Tet-ON dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid

    #195622

    Purpose

    Sleeping Beauty Tet-ON plasmid for induced expression of dCasphi-ZIM3 CRISPRi construct

    Depositor

    Tonia Rex

    Insert

    dCasphi
    

    Use

    Tags

    C-Myc (NLS), HA tag, and ZIM3

    Expression

    Mammalian

    Mutation

    P749A and P753A

    Promoter

    Tight TRE promoter; Tet-ON (doxycycline)

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. (915) SB EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid

    #195623

    Purpose

    Sleeping Beauty EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct

    Depositor

    Tonia Rex

    Insert

    dCasphi
    

    Use

    Tags

    C-Myc (NLS), HA tag, and ZIM3

    Expression

    Mammalian

    Mutation

    P749A and P753A

    Promoter

    EF1a (Mlu1/EcoR1)

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP)

    Plasmid

    #218640

    Purpose

    Gateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    delPYD (I125M-END)

    Promoter

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE FL NAP1 STOP C' EGFP IRES Blast

    Plasmid

    #179102

    Purpose

    Expresses untagged full length NAP1

    Depositor

    Alicia Pickrell

    Article

    Paul et al J Cell Biol. 2024 Feb 5;223(2):e20230308

    Insert

    Nak associated protein 1 (AZI2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Anti-Histone H4-dimethyl-Arg3 [N309A/21R]

    Plasmid

    #206552

    Purpose

    Mammalian Expression Plasmid of anti-Histone H4-dimethyl-Arg3 (Human). Derived from hybridoma N309A/21.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Histone H4-dimethyl-Arg3 (Homo sapiens) recombinant Mouse monoclonal antibody (H4C14 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]

    Plasmid

    #196883

    Purpose

    Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    roxSTOProx-ZsGreen-bGH
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBactin-DIO-inv[roxSTOProx-ZsGreen-bGH]

    Plasmid

    #196886

    Purpose

    Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    roxSTOProx-ZsGreen-bGH
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. EK0441 CMV-TO-BFPmut-stop-EGFP.t2 (FLP-IN)

    Plasmid

    #191161

    Purpose

    Expression of a trigger sequence from EGFP CDS in 3' UTR context in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    EGFP.t2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p8322 pDONR ATG-YAP1 S94A-Stop

    Plasmid

    #184526

    Purpose

    Subcloning YAP1 S94A

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

    Insert

    YAP1 S94A (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    S94A

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p8321 pDONR ATG-YAP1 S127A-Stop

    Plasmid

    #184525

    Purpose

    Subcloning YAP1 S127A

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

    Insert

    YAP1 S127A (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    S127A

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAc5.1C-5Box-73-Fluc-STOP-CG10011-SV40_D

    Plasmid

    #146157

    Purpose

    Insect Expression of 5BoxB-73-Fluc-CG10011-3UTR

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al RNA. 2009 Jan . 15(1):21-32.

    Insert

    5BoxB-73-Fluc-CG10011-3UTR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSB700 lenti gRNA Blasto_TtoC and hairpin extension

    Plasmid

    #167920

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAc5.1C-5Box-7-Fluc-STOP-CG10011-SV40_D

    Plasmid

    #146156

    Purpose

    Insect Expression of 5BoxB-7-Fluc-CG10011-3UTR

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al RNA. 2009 Jan . 15(1):21-32.

    Insert

    5BoxB-7-Fluc-CG10011-3UTR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. C512-E01: attB5r-meGFP-nostop-attB1r

    Plasmid

    #162940

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal eGFP fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    monomeric enhanced Green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. C511-E15: attB5r-ffluc2-nostop-attB1r

    Plasmid

    #162939

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Firefly luciferase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. C512-E11: attB5r-mTFP1-nostop-attB1r

    Plasmid

    #162942

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal mTFP1 fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    monomeric Teal fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. C514-E10: attB5r-RU5u-HA-nostop-attB1r

    Plasmid

    #162952

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal HA-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    HA epitope tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. C514-E24: attB5r-RU5u-V5-nostop-attB1r

    Plasmid

    #162959

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal V5-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    V5 epitope tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. C512-E34: attB5r-iRFP670-nostop-attB1r

    Plasmid

    #162947

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal iRFP670 fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    far-red iRFP670 fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. C512-E29: attB5r-mKate2-nostop-attB1r

    Plasmid

    #162946

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal mKate2 fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    monomeric Kate2 fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. C512-E15: attB5r-mVenus-nostop-attB1r

    Plasmid

    #162943

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal mVenus fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    monomeric Venus fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. CFP(159-238)Stop in pcDNA1/amp

    Plasmid

    #60893

    Purpose

    This vector attached CFP(159-238) to the C-terminus of a protein.

    Depositor

    Catherine Berlot

    Insert

    CFP(159-238)Stop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Asn-164 was changed to His

    Promoter

    CMV

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA

    Plasmid

    #63166

    Purpose

    GAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-HA"
    

    Use

    Tags

    N-myristoylation

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pOTTC1181 - pmU6(loxP-STOP-loxP) BbsI gRNA

    Plasmid

    #113160

    Purpose

    A plasmid for cloning Cre-dependent guide RNAs using a modified mouse U6 promoter containing loxP

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. (565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP

    Plasmid

    #163030

    Purpose

    Expression of CRISPRi with gSTOP gRNA

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    AAV

    Tags

    KRAB, SV40 pA, and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Alb-AAT

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pJFRC164-21XUAS-KDRT>-dSTOP-KDRT>-myr::RFP

    Plasmid

    #32141

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    KDRT> STOP STOP KDRT>
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pHAGE pGK LC3Flux (HaloTag7-mGFP-LC3-STOP)

    Plasmid

    #215015

    Purpose

    Express an autophagy (LC3) reporter in mammalian cells (untested)

    Depositor

    Wade Harper

    Insert

    MAP1LC3B (Map1lc3b Rat)
    

    Use

    Lentiviral

    Tags

    HaloTag(7)-mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

    Plasmid

    #101297

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-906 of alpha-Catenin

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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44. pAAV-TRE-DIO-eGFP-2A-TetanusToxin (Cre-OFF)

    Plasmid

    #166611

    Purpose

    Encodes Cre-inactivated GFP-2A-Tetanus toxin light chain under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    EGFP-2A-Tetanus Toxin
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP

    Plasmid

    #32149

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    FRT> STOP STOP FRT>
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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46. pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

    Plasmid

    #63168

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-V5"
    

    Use

    Tags

    N-myristoylation

    Expression

    Insect

    Mutation

    Promoter

    GAL4-responsive UAS hsp70

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid

    #215010

    Purpose

    Express a Mitophagy reporter in mammalian cells (untested)

    Depositor

    Wade Harper

    Insert

    pSu9 (Presequence of Fo-ATPase subunit 9)
    

    Use

    Lentiviral

    Tags

    HaloTag(7)-mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. EK0307 CMV-TO-BFPmut-stop-3UTR(Bdnf) (FLP-IN)

    Plasmid

    #191145

    Purpose

    Expression of a synthetic construct with 3' UTR of murine Bdnf in the 3' UTR of an inactive BFP in mammalian cells.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    3UTR(Bdnf)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pXL-BacII-CAG-loxP-STOP-loxP-Venus

    Plasmid

    #170520

    Purpose

    Plasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

    Insert

    CAG-loxP-STOP-loxP-Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCAG-DIO-inv[roxSTOProx-EB3 mNeonGreen-bGH]

    Plasmid

    #196885

    Purpose

    Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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