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We narrowed to 13,710 results for: cas9

Showing: 521 - 540 of 13710 results

  3. pLenti_dCas9-2xAM

    Plasmid
    #92220
    Purpose
    Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites
    Depositor
    Hodaka Fujii
    Article
    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do
    Inserts
    Sp-dCas9-2xAM tag
    gRNA to be inserted into Bbs I sites
    Use
    CRISPR and Lentiviral
    Tags
    2xAM tag
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    CBh and U6
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    92220-plasmid-map-sequence-id-181084

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  12. pAWPSG-Po-nCas9-BE

    Plasmid
    #195740
    Purpose
    Express APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coli
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Po (phoenol-inducible promoter)
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195740-plasmid-map-sequence-id-386428

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  14. LentiRCas9-CUG

    Plasmid
    #104183
    Purpose
    Lentiviral transfer vector that carries U6-driven sgRNA targeting CUG repeats using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)
    Depositor
    Eugene Yeo
    Article
    Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do
    Insert
    U6-CUGsgRNA, EFS-PIN-dCas9
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    104183-plasmid-map-sequence-id-201743

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  15. pcDNA-dCas9-HA

    Plasmid
    #61355
    Purpose
    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    61355-plasmid-map-sequence-id-99781

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  18. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104911
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    104911-plasmid-map-sequence-id-269032

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  20. pH-nCas9-PPE

    Plasmid
    #140447
    Purpose
    For plant prime editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.
    Insert
    nCas9(H840A)-M-MLV
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    H840A for Cas9; D200N, T306K, W313F, T330P and L…
    Promoter
    maize Ubiquitin-1, OsU3
    Available since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140447-plasmid-map-sequence-id-296176

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Showing: 521 - 540 of 13710 results