Addgene

We narrowed to 1,814 results for: pCAG

Showing: 521 - 540 of 1814 results

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pCAG-mCherry-ATG14

Plasmid

#203567

Purpose

Mammalian expression of mCherry tagged ATG14

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG14 (ATG14 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6His-mISG15

Plasmid

#12445

Depositor

Dong-Er Zhang

Article

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

ISG15

Use

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pCAGGS-PIF3-mEGFP

Plasmid

#100283

Purpose

Expresses PIF3 (1-100 aa) fused with mEGFP in mammalian cells

Depositor

Kazuhiro Aoki

Article

Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

Insert

Phytochrome interacting factor 3 (PIF3 Mustard Weed)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

N-terminus (1-100 aa) of PIF3 is used

Promoter

CAG promoter

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Myc-Oct3/4-IP

Plasmid

#13460

Depositor

Shinya Yamanaka

Article

Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

Insert

Oct3/4 (Pou5f1 Mouse)

Use

Tags

Myc (6x)

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG14

Plasmid

#203566

Purpose

Mammalian expression of GST tagged ATG14

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG14 (ATG14 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph18-eGFP-CCR5TC

Plasmid

#135529

Purpose

Encodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph18

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Flp-DOG

Plasmid

#80084

Purpose

Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

Flp-DOG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP-N-IFT80

Plasmid

#218731

Purpose

Expresses C-terminally EGFP-tagged IFT80 in mammalian cells

Depositor

Kazuhisa Nakayama

Insert

IFT80 (IFT80 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2Δ751-813aa

Plasmid

#163603

Purpose

Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

The LIMK-binding region (751-813 aa) is deleted

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3xFLAG-Rbfox2

Plasmid

#213726

Purpose

Mammalian cell expression vector for overexpression of Rbfox2 in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

Rbfox2 (Rbfox2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Gal4DBD-GBP2

Plasmid

#49439

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GBP2-Gal4 DNA binding domain fusion protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-spCas9-N57

Plasmid

#169920

Purpose

Expression of SpCas9-N57 fusion protein to enhace site-specific DNA integration.

Depositor

Zhili Rong

Article

Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

Insert

SpCas9-N57

Use

CRISPR

Tags

Expression

Mutation

N57 is the PAI domain of SB100X

Promoter

CAG

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph15-eGFP-CCR5TC

Plasmid

#135528

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CD4-myc

Plasmid

#58537

Purpose

Expresses human CD4 with a C-terminal myc tag in mammalian cells

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

CD4 (CD4 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only
pCAG vGlut1 GCaMP5G

Plasmid

#105008

Purpose

Presynaptic calcium sensor

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

SLC17A7

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Roxed-Cre

Plasmid

#51273

Purpose

Component of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by Dre

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

Roxed-Cre

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace2_D92N_E199V-mNeon

Plasmid

#129256

Purpose

Express Ace2_D92N_E199V-mNeon in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mNeon

Use

Tags

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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hGli3 NTOTAL in AINGpCAGGS

Plasmid

#121973

Purpose

medium Gli3 transcriptional repressor

Depositor

James Briscoe

Article

Ulloa et al Curr Biol. 2007 Mar 20;17(6):545-50. doi

Insert

hGli3 NTOTAL (GLI3 Human)

Use

Chick electroporation

Tags

5xmyc and Linker A IRES NLS GFP pCAGGS

Expression

Mammalian

Mutation

C terminus deletion from zinc finger domain

Promoter

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-intron

Plasmid

#177804

Purpose

An intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

intron

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-GST FNIP2

Plasmid

#164982

Purpose

FNIP2 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

FNIP2 (FNIP2 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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