We narrowed to 15,050 results for: pET

1. pET28a MISTIC

   Plasmid

   #85999

   Purpose

   bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

   Depositor

   Wolfram Antonin

   Article

   Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

   Insert

   N/A
   

   Tags

   HIS6 and MISTIC

   Expression

   Bacterial

   Mutation

   codon optimzed for bacterial expression

   Promoter

   T7

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET31b-T7-Spinach2

   Plasmid

   #79783

   Purpose

   Expresses Spinach2 under a T7 promoter

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Insert

   T7-tRNA-Spinach2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LCC-ICCG_pET21b

   Plasmid

   #215408

   Purpose

   Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   LCC-ICCG
   

   Expression

   Bacterial

   Mutation

   F243I/D238C/S283C/Y127G

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET20b_Pa-PiuA

   Plasmid

   #128955

   Purpose

   expresses Pseudomonas aeruginosa PiuA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

   Insert

   iron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)
   

   Tags

   6xHis and pelB leader

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed

   Promoter

   T7

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-cMSPdH5

   Plasmid

   #199771

   Purpose

   Expresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSPdH5
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   external His-Tags flanking the C and N termini

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET20b_Pa-PirA

   Plasmid

   #128956

   Purpose

   expresses Pseudomonas aeruginosa PirA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

   Insert

   ferric enterobactin receptor PirA (pirA Pseudomonas aeruginosa PAO1, Synthetic)
   

   Tags

   6xHis and pelB leader

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed

   Promoter

   T7

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-LIC

   Plasmid

   #26094

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Tags

   6xHis and Thrombin cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. Ube1/PET21d

   Plasmid

   #34965

   Depositor

   Cynthia Wolberger

   Article

   Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10.

   Insert

   Ube1 (UBA1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-cMSP26

   Plasmid

   #199769

   Purpose

   Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSP26
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-cMSPdH4-6

    Plasmid

    #199766

    Purpose

    Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH46
    

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    internal His-Tag

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDB179-HotPETase

    Plasmid

    #218701

    Purpose

    Encodes codon optimized HotPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    HotPETase
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET29b-H6_Streptavidin_sfGFP

    Plasmid

    #124296

    Purpose

    His6-tagged Streptavidin GFP fusion protein

    Depositor

    Mark Arbing

    Insert

    His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
    

    Tags

    His6 and T7 tags

    Expression

    Bacterial

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDB179-TurboPETase

    Plasmid

    #218693

    Purpose

    Encodes codon optimized TurboPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    TurboPETase
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28HIS-hAPE1

    Plasmid

    #70757

    Purpose

    bacterial expression of human APE1

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    APEX1 (APEX1 Human)
    

    Tags

    6-HIS

    Expression

    Bacterial

    Promoter

    T7/lac operon

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET24TEV-tSHIP1

    Plasmid

    #183770

    Purpose

    Expresses C-terminal truncated SHIP1 (tSHIP1)with C-terminal TEV and 6HIS tags from pET24 backbone

    Depositor

    William Kerr

    Article

    Sudan et al Front Immunol. 2022 May 4;13:830961. doi

    Insert

    SHIP1 (INPP5D Human)
    

    Tags

    6HIS and Tobacco Etch Virus (TEV) Cleavage site

    Expression

    Bacterial

    Mutation

    includes aa1 to aa883 only

    Promoter

    T7 lac

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET30-2-GPD1L

    Plasmid

    #83912

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glycerol-3-phosphate dehydrogenase 1-like (GPD1L Human)
    

    Tags

    6x His tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETM6-mCherry

    Plasmid

    #66534

    Purpose

    Expresses mCherry under the control of the Concensus T7 Promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Consensus T7

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET15-UbE2D1

    Plasmid

    #15782

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
    

    Tags

    6x His, T7 tag, and Xpress tag (enterokinase clea…

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET30a-AlkB

    Plasmid

    #79050

    Purpose

    Express wild type AlkB protein in E. coli BL21(DE3)

    Depositor

    Tao Pan

    Article

    Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

    Insert

    AlkB (alkB E.coli)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    A truncated AlkB with deletion of the amino (N)-t…

    Promoter

    T7

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a_T7-ARG1

    Plasmid

    #106473

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG1
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge