We narrowed to 19,553 results for: INO

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STARR-seq luciferase validation vector_ORI_empty

Plasmid

#99297

Purpose

Luciferase validation vector; empty vector without insert to determine baseline

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pJRH-1365 LV EF1a-CD28 IRES-EGFP

Plasmid

#201921

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD28

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD28 (CD28 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam p53 R273H

Plasmid

#16439

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 R273H (TP53 Human)

Expression

Mammalian

Mutation

R273H

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Chk1-Flag

Plasmid

#22894

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Chk1 (CHEK1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam p53 R248W

Plasmid

#16437

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 R248W (TP53 Human)

Expression

Mammalian

Mutation

R248W

Available Since

Aug. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pGM1190

Plasmid

#69994

Purpose

OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aac
tsr
rep
sso
TipA promoter
dso

Expression

Bacterial

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pET-BiFC

Plasmid

#87856

Purpose

pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Inserts

Encodes for MCS & C-term half of mVenus
Encodes for MCS & N-term half of mVenus

Tags

BiFC C-terminal fragment

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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AnkyrinG-190-GFP

Plasmid

#31059

Depositor

Vann Bennett

Article

Mohler et al J Biol Chem. 2002 Mar 22. 277(12):10599-

Insert

Ankyrin G (Ank3 Rat)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 28, 2012

Availability

Academic Institutions and Nonprofits only

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hMLKL-Venus

Plasmid

#106078

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL-Venus (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

Venus

Expression

Mammalian

Promoter

Dox-inducible

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_GFP 150TAG 6xHis

Plasmid

#140015

Purpose

Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Tags

6xHis

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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CRISPR-StAR4BN (Lenti)

Plasmid

#222692

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

PGK without BsmBI cutsite

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-GPHN-FingR-mGold2s-CCR5TC

Plasmid

#231785

Purpose

Visualization of inhibitory post-synapse using GPHN-FingR-mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

GPHN-FingR-mGold2s-CCR5TC

Expression

Mammalian

Promoter

Actin promoter with CMV enhancer

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-42B3mutAC2(Ser2ph)-SNAP

Plasmid

#174876

Purpose

PiggyBac transposon vector for expression of RNAPII Ser2ph mintbody

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

RNAPII Ser2ph mintbody-SNAP

Use

Piggybac vector

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTDH3-dCas9-Mxi1

Plasmid

#46921

Purpose

Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoter

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9-Mxi1

Use

CRISPR

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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Lenti-AsCpf1-Blast

Plasmid

#84750

Purpose

Expresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

Depositor

Hyongbum Kim

Article

Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

AsCpf1

Use

CRISPR and Lentiviral

Tags

NLS-3xHA (C terminal on insert)

Expression

Mammalian

Promoter

EFS-NS

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS-N-HA (murine)

Plasmid

#130916

Purpose

Expresses murine cGAS N terminus(aa1-147)-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

murine cGAS N terminus

Tags

HA

Expression

Mammalian

Mutation

murine cGAS N terminus (aa 1-147)

Promoter

CMV

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam p53 R249S

Plasmid

#16438

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 R249S (TP53 Human)

Expression

Mammalian

Mutation

R249S

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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pPBbsr-AcGFP1-ORP9-PH

Plasmid

#214270

Purpose

Mammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

AcGFP1-ORP9(1-111) (Osbpl9 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pYES2-gRNA-hyg-MCS

Plasmid

#107734

Purpose

E. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.

Depositor

Sheng Yang

Article

Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

Insert

X-3 site gRNA expression cassettes

Use

CRISPR

Expression

Yeast

Promoter

SNR52p

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO mScarlet

Plasmid

#131002

Purpose

Double floxed mScarlet under the control of Ef1a promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV1 and AAV5

Insert

mScarlet

Use

AAV

Promoter

Ef1a

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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