Addgene

We narrowed to 21,419 results for: ESC;

Showing: 8101 - 8130 of 21419 results

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pXTK056 [Con-Pro_glpT-Ecoli_PartType8]

Plasmid

#229293

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_glpT-Ecoli_PartType8

Depositor

Michael Springer

Article

Soltysiak et al ACS Synthetic Biology. 2025. doi: 10.102

Insert

glpT Promoter (E coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK035 [Origin_RK2-oriT_PartType3]

Plasmid

#229272

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_RK2-oriT_PartType3

Depositor

Michael Springer

Article

Soltysiak et al ACS Synthetic Biology. 2025. doi: 10.102

Insert

RK2-oriT Origin

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK037 [Con-Pro_glpT-Ecoli_PartType5]

Plasmid

#229274

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_glpT-Ecoli_PartType5

Depositor

Michael Springer

Article

Soltysiak et al ACS Synthetic Biology. 2025. doi: 10.102

Insert

glpT Promoter (E coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK025 [Origin_RK2-oriT_PartType1]

Plasmid

#229262

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_RK2-oriT_PartType1

Depositor

Michael Springer

Article

Soltysiak et al ACS Synthetic Biology. 2025. doi: 10.102

Insert

RK2-oriT Origin

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pKrev-CGI

Plasmid

#225568

Purpose

packaging plasmid for lentivector production

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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p48.unipolyA.dNGFR.mCMV.PGK.eGFP.Gem1.WPRE

Plasmid

#226767

Purpose

vector with NGFR and GFP

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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pM-mKate2

Plasmid

#228629

Purpose

Mammalian expression of mKate2 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mKate2

Use

Tags

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pM-LSSmOrange

Plasmid

#228619

Purpose

Mammalian expression of LSSmOrange and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

LSSmOrange

Use

Tags

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pTBY1-T4gp61/41 fusion

Plasmid

#228210

Purpose

Expression in BL21(DE3) of T4 primase and helicase fusion with 24 amino acid linker

Depositor

Stephen Benkovic

Article

Manosas et al Nat Chem Biol. 2009 Dec;5(12):904-12. do

Insert

61/41

Use

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pTYB1-T4gp47

Plasmid

#228212

Purpose

Expression in BL21(DE3) of T4 SbcD-like subunit of palindrome specific endonuclease

Depositor

Stephen Benkovic

Article

Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

Insert

47 (47 Escherichia phage T4)

Use

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pR-mOrange2

Plasmid

#228608

Purpose

Mammalian expression of mOrange2, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mOrange2

Use

Tags

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pR-mBeRFP

Plasmid

#228601

Purpose

Mammalian expression of mBeRFP, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mBeRFP

Use

Tags

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pR-mTagBFP2

Plasmid

#228596

Purpose

Mammalian expression of mTagBFP2, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mTagBFP2

Use

Tags

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pR-LSSmOrange

Plasmid

#228600

Purpose

Mammalian expression of LSSmOrange, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

LSSmOrange

Use

Tags

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pR-mTFP1

Plasmid

#228602

Purpose

Mammalian expression of mTFP1, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mTFP1

Use

Tags

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pR-mRuby3

Plasmid

#228609

Purpose

Mammalian expression of mRuby3, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mRuby3

Use

Tags

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pM-mBeRFP

Plasmid

#228620

Purpose

Mammalian expression of mBeRFP and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mBeRFP

Use

Tags

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pM-mTFP1

Plasmid

#228621

Purpose

Mammalian expression of mTFP1 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mTFP1

Use

Tags

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pM-CyOFP1

Plasmid

#228623

Purpose

Mammalian expression of CyOFP1 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

CyOFP1

Use

Tags

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pM-mOrange2

Plasmid

#228627

Purpose

Mammalian expression of mOrange2 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mOrange2

Use

Tags

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pM-mRuby3

Plasmid

#228628

Purpose

Mammalian expression of mRuby3 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Insert

mRuby3

Use

Tags

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-a(+)-6xHis-miRFP(-5)-HO1

Plasmid

#219424

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Inserts

miRFP682
HO1

Use

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Contains Ribosomal Binding Site for expression of…

Promoter

T7

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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PtetA with pBELO_L6-PT3-T4_CAMR_TAN+

Plasmid

#225644

Purpose

tetA promoter expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

Depositor

Andre Sanches Ribeiro

Article

Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

Insert

tetR/tetA

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

tetA promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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PLac0301 with pBELO_L6-PT3-T4_CAMR_TAN+

Plasmid

#225643

Purpose

Lac0301 promoter expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

Depositor

Andre Sanches Ribeiro

Article

Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

Insert

LacO3O1

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

LacO3O1 promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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ParaBAD with pBELO_L6-PT3-T4_CAMR_TAN+

Plasmid

#225642

Purpose

araBAD promoter expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

Depositor

Andre Sanches Ribeiro

Article

Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

Insert

araBAD

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

BAD promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Ub-LPNTG-pET

Plasmid

#221850

Purpose

Expresses Ub with sortase motif and His tag

Depositor

Artur Krężel

Article

Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

Insert

Ubiquitin

Use

Tags

Expression

Bacterial

Mutation

Arg74ProLeuThr

Promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GB4650

Plasmid

#215265

Purpose

TU for the copper regulated expression of the PhiC31 recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:PhiC31:tNos

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GB4652

Plasmid

#215266

Purpose

TU for the copper regulated expression of the flippase (FLP) recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:FLP:TNOS

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GB1203

Plasmid

#215369

Purpose

TU for the expression of the silencing suppressor P19 driven by the 35s promoter

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

35s:P19:tNos

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pWR95(URA3)

Plasmid

#203917

Purpose

BLINCAR plasmid for gene deletion, URA3 target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

URA3 upstream homology
URA3 downstream homology

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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