We narrowed to 24,440 results for: lars

1. pJEP228-Lenti-CMV-GFP-GluN2B-WPRE-pA

   Plasmid

   #84277

   Purpose

   pLenti vector backbone designed to express GFP tagged GluN2B from a CMV promoter.

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   GluN2B
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   CMV

   Available since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJEP227-Lenti-CMV-GFP-GluN2A-WPRE-pA

   Plasmid

   #84276

   Purpose

   pLenti vector backbone designed to express GFP tagged GluN2A from a CMV promoter.

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   GluN2A
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTX1-RNA0

   Plasmid

   #126040

   Purpose

   In-vitro-transcription of control RNA0 in NMR tube for "Systems NMR" analysis

   Depositor

   Frédéric Allain

   Article

   Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

   Insert

   RNA0
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. hTMEM115-TEV-3xHA-P2A-mScarlet HDR template

   Plasmid

   #214998

   Purpose

   Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   hTMEM115-TEV-3xHA-P2A-mScarlet HDR template (TMEM115 Human)
   

   Use

   Hdr template

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   None

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template

   Plasmid

   #214999

   Purpose

   Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mNG for cell sorting.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template (TMEM115 Human)
   

   Use

   Hdr template

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   None

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOZC-PALB2

   Plasmid

   #133552

   Purpose

   PALB2 coding sequence in pOZ-FH-C C-terminal Flag, His tagging retroviral vector.

   Depositor

   David Livingston

   Article

   Xia et al Mol Cell. 2006 Jun 23. 22(6):719-29.

   Insert

   PALB2 (PALB2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-AP.Agmat.1-GFP-WPRE

   Plasmid

   #211489

   Purpose

   expresses GFP under artificial promoter Agmat

   Depositor

   Georg Keller

   Article

   O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bxb1-surrogate-reporter

   Plasmid

   #65624

   Purpose

   Fluorescent reporter to monitor the activty of Bxb1

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   attP
   

   Use

   Bxb1

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIS.MTRIA-PGE

   Plasmid

   #184617

   Purpose

   For expression of a fluorescent sensor for prostaglandin E2 factor in mammalian cells

   Depositor

   Hiroshi Hibino, Daisuke Ino

   Article

   Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

   Insert

   MTRIA-PGE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFH103

    Plasmid

    #128208

    Purpose

    crRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    crRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOS028E: Entry vector for: Mitycam calcium sensor (mitochondrial)

    Plasmid

    #163065

    Purpose

    Entry vector for: Mitycam calcium sensor (mitochondrial)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Mitycam
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCSIIpuro-4493NES(Booster-PKA)

    Plasmid

    #184710

    Purpose

    PKA biosensor

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    Booster-PKA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEYFP-N1-HsSmg7_H

    Plasmid

    #146476

    Purpose

    Mammalian Expression of HsSmg7

    Depositor

    Elisa Izaurralde

    Article

    Unterholzner et al Mol Cell. 2004 Nov 19. 16(4):587-96.

    Insert

    HsSmg7 (SMG7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation G2560A (V854I) and three …

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. LV anti-FITC-LaG16-Y186F-T2A-BFP

    Plasmid

    #194931

    Purpose

    LV construct encoding Y186F mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y186F)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-4T-3-SH3BP1

    Plasmid

    #136647

    Purpose

    Expression of SHBP1-GST fusion protein in E. coli

    Depositor

    Karl Matter, Maria Balda

    Article

    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

    Insert

    SH3BP1 (SH3BP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pfMDH_WT

    Plasmid

    #67721

    Purpose

    Expresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pG-HIV-LRT

    Plasmid

    #104592

    Purpose

    Standard for qPCR

    Depositor

    Kristine Yoder

    Article

    Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

    Insert

    HIV-1 late reverse transcripts
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDisplay-Gncp-iGluSnFR

    Plasmid

    #107337

    Purpose

    Noncircularly permutated (ncp) variant of iGluSnFR. Membrane-displayed.

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

    Insert

    GltI based and noncircularly permutated glutamate biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AlphaC

    Plasmid

    #124525

    Purpose

    pColE1_AlphaC

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Aurein1.2_(+36GFP)-Cre

    Plasmid

    #71748

    Purpose

    Aurein1.2+36GFP-Cre fusion expression in E. Coli

    Depositor

    David Liu

    Article

    Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

    Insert

    (GGS)9_Aurein1.2+36GFP-Cre
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAV-U6+27-Tornado-F30-TAR Variant-1

    Plasmid

    #129406

    Purpose

    circular TAR Variant-1

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    circular F30-TAR Variant-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAS9-mCherry-TUBB

    Plasmid

    #66942

    Purpose

    CRISPaint target selector TUBB

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA TUBB
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a-SdrG_N2N3-HIS-ybbr

    Plasmid

    #101238

    Purpose

    SdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    SdrG_N2N3
    

    Use

    Tags

    H3V 3C, HIS, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUC57-Kan_US71_APikL1

    Plasmid

    #123646

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    US71_APikL1
    

    Use

    Golden gate

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GEV 2

    Plasmid

    #12616

    Depositor

    John Louis

    Article

    Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

    Insert

    GB1 domain of protein G as N terminal tag, with IgT antigen as insert (SV40gp6 SV40)
    

    Use

    Tags

    G protein and His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

26. pCAG-HA-NLGN3 R451C

    Plasmid

    #59319

    Purpose

    Expression plasmid of HA-tagged human Neuroligin 3 harboring an R451C mutation

    Depositor

    Peter Scheiffele

    Article

    Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

    Insert

    NLGN3 R451C (NLGN3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Arginine 451 to Cysteine (R451C mutation)

    Promoter

    CAG

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCDNA HA FAK Y925F

    Plasmid

    #50509

    Depositor

    Kenneth Yamada

    Article

    Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

    Insert

    protein tyrosine kinase, mutated
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y925F

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

28. MTK3_010

    Plasmid

    #123725

    Purpose

    Encodes Tet-On 3G transactivator protein as a Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    TetOn3G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHR_UAS-tTS-P2A-GFP_PGK-BFP

    Plasmid

    #133808

    Purpose

    inducible expression of tTS and GFP with BFP marker in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2018 Jul 13;361(6398):156-162.

    Insert

    tTS-P2A-EGFP-PGK-BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOGG026

    Plasmid

    #113992

    Purpose

    Level 1 golden gate cloning vector for low copy number stable environmental gene expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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