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We narrowed to 89,701 results for: MAL

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Showing: 10801 - 10840 of 89701 results

  9. MSP2443

    Plasmid
    #72252
    Purpose
    Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72252-plasmid-map-sequence-id-207329

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  12. pCMV-RVR-A3A-Y130F

    Plasmid
    #193658
    Purpose
    Expression of base editor RVR-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    RVR-dLbCas12a-hA3A-Y130F, EGFP
    Expression
    Mammalian
    Mutation
    dLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-Y130F
    Promoter
    CMV
    Available Since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    193658-plasmid-map-sequence-id-426532

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  13. pCMV-RVR-A3A-N57G

    Plasmid
    #193657
    Purpose
    Expression of base editor RVR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    RVR-dLbCas12a-hA3A-N57G, EGFP
    Expression
    Mammalian
    Mutation
    dLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-N57G
    Promoter
    CMV
    Available Since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193657-plasmid-map-sequence-id-422142

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  15. pAAV-UBC-S1-GCaMP6f

    Plasmid
    #160722
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S1-GCaMP6f
    Use
    AAV
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available Since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160722-plasmid-map-sequence-id-315521

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  19. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139089
    Purpose
    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139089-plasmid-map-sequence-id-273885

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  23. CIRTS-control

    Plasmid
    #213788
    Purpose
    CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Article
    Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:
    Insert
    CIRTS-Calm3-U6-control gRNA
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    Syn1, U6
    Available Since
    Feb. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    213788-plasmid-map-sequence-id-424349

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  31. pBa-tdTomato-flag-BicD2 594-FKBP

    Plasmid
    #64205
    Purpose
    Bicaudal D protein aa1-594 (excluding start Met) with N-terminal tandem dimer Tomato and flag tags and C-terminal FKBP
    Depositor
    Gary Banker, Marvin Bentley
    Article
    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.
    Insert
    Bicaudal D protein (Bicd2 Mouse)
    Tags
    FKBP, flag, and tdTomato
    Expression
    Mammalian
    Mutation
    no start methionine, aa1-594
    Promoter
    chicken Beta Actin
    Available Since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    64205-plasmid-map-sequence-id-107603

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  36. pLenti-xBE4Gam-P2A-Puro

    Plasmid
    #112676
    Purpose
    Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)
    Depositor
    Lukas Dow
    Article
    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419
    Insert
    xCas9(3.7)-BE4Gam
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…
    Promoter
    EF1s
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    112676-plasmid-map-sequence-id-217196

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Showing: 10801 - 10840 of 89701 results