We narrowed to 17,597 results for: She

1. KG371-GFP-mios

   Plasmid

   #87052

   Purpose

   mammalian expression of mios

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   mios (MIOS Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKD279

   Plasmid

   #125099

   Purpose

   Expression of SO_4388(1-130aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   SO_4388(REC)-psdR(DBD)137
   sfgfp
   tetR
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. U6_ ATM_G101_sgRNA_CAG_St1Cas9_CNRZ1066_v2

   Plasmid

   #214814

   Purpose

   A single vector containing a CAG-driven Cas9 variant from S. thermophilus recognizing a consensus NNACAA PAM (St1Cas9 CNRZ1066 v2) and its U6-driven sgRNA targeting human ATM

   Depositor

   Yannick Doyon

   Insert

   St1Cas9 CNRZ1066 v2 targeting ATM (ATM Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FLAG-FLJ20309

   Plasmid

   #15360

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

   Insert

   FLJ20309 106-544residues (INO80D Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pv6_dPC_Chromo_TAF3_Phd

   Plasmid

   #179403

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_Chromo - TAF3_Phd

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   dPC_Chromo - TAF3_Phd
   

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD-SKW-HHL

   Plasmid

   #190632

   Purpose

   Bacterial expression of genetically encoded potassium indicator SKW-HHL

   Depositor

   Robert Campbell

   Article

   Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

   Insert

   SKW-HHL
   

   Expression

   Bacterial

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-deltaA1-VWF

   Plasmid

   #124823

   Purpose

   Expresses multimeric human VWF protein without A1 domain. This construct has no tag.

   Depositor

   Sriram Neelamegham

   Article

   Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

   Insert

   VWF without A1 domain (VWF Human)
   

   Expression

   Mammalian

   Mutation

   Deleted A1 domain

   Promoter

   CMV

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLJC6-Flag CASTOR1

   Plasmid

   #84496

   Purpose

   Lentiviral expression of CASTOR1

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

   Insert

   CASTOR1 (GATSL3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   sestrin2

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

9. PD-L1-miSFIT-4x

   Plasmid

   #124684

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-L1 (CD274 Human)
   

   Expression

   Mammalian

   Promoter

   minimal CMV

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSR357.9

    Plasmid

    #125105

    Purpose

    Expression of narL(1-129aa)-ydfI(124-213aa) chimera under PLtetO-1, output Promoter PydfJ115

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL(REC)-ydfI(DBD)129
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRK5-MYC-KPTN

    Plasmid

    #87043

    Purpose

    mammalian expression of KPTN

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    KPTN (KPTN Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PKM-IRES-hrGFP

    Plasmid

    #73405

    Purpose

    Mammalian expression of mouse PKM and hrGFP

    Depositor

    Raymond Swanson

    Article

    Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

    Insert

    PKM (Prkcz Mouse)
    

    Tags

    3xFLAG and pIRES-hrGFP-2a

    Expression

    Mammalian

    Mutation

    truncated, constitutively active form

    Promoter

    CMV

    Available Since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

13. PLK3 335-646-pEGFP

    Plasmid

    #23267

    Depositor

    Wei Dai

    Article

    Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

    Insert

    PLK3 (PLK3 Human)
    

    Tags

    pEGFP

    Expression

    Mammalian

    Mutation

    aa 335-646

    Available Since

    Dec. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pGL3-24p3/LCN2-282kB mut-LUC

    Plasmid

    #46867

    Purpose

    murine 24p3 minimal promoter with NF-kB mutation as a luciferase reporter

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    24p3/lipocalin 2 proximal promoter
    

    Use

    Luciferase

    Mutation

    NF- kB site is mutated (see comments)

    Promoter

    24p3 promoter (inactive)

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pGL3-24p3/LCN2-282C/EBP mut-LUC

    Plasmid

    #46868

    Purpose

    murine 24p3 minimal promoter with C/EBP mutation k as a luciferase reporter

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    24p3/lipocalin 2 proximal promoter
    

    Use

    Luciferase

    Mutation

    CEBP site is mutated (see comments)

    Promoter

    24p3 promoter (inactive)

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pEPQDKN0761

    Plasmid

    #185630

    Purpose

    Modified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T which targets phytoene desaturase (PDS) of Nicotiana benthamiana, functionally equivalent to pEE393

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Dok1-EGFP

    Plasmid

    #174194

    Purpose

    Expresses Dok1 in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Yu et al Nat Commun. 2015 Oct 28;6:8672. doi: 10.

    Insert

    DOK1 (DOK1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBabe_puro_DEST_Flag_PRKCI

    Plasmid

    #45263

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    PKCI (PRKCI Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pGEX2T-p19Ink4d

    Plasmid

    #52222

    Purpose

    bacterial expression of p19Ink4d

    Depositor

    Martine Roussel

    Article

    Forget et al Cell Cycle. 2008 Dec;7(23):3737-46. Epub

    Insert

    Ink4d (Cdkn2d Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

20. OVA-miSFIT-2xPerfect

    Plasmid

    #124673

    Purpose

    Tuning expression of cytosolic Ovalbumin coupled to EGFP

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    Cyto-Ovalbumin
    

    Expression

    Mammalian

    Mutation

    Truncated OVA for cytoplasmic expression

    Promoter

    minimal CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO

    Plasmid

    #68357

    Purpose

    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.

    Depositor

    Jannik Elverløv-Jakobsen

    Inserts

    5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9
    FlpO recombinase
    

    Use

    CRISPR

    Tags

    mTurquoise2 (BFP)

    Expression

    Mammalian

    Promoter

    U6 and synthetic Probasin ARRx2

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-hSyn-mCherry-T2A-miniSOG-C-Far

    Plasmid

    #193915

    Purpose

    Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Farnesylation signal sequence and Flag tag

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJN601

    Plasmid

    #59790

    Purpose

    zf1::yfp fusion with floxed unc-119(+) within an intron of yfp. For creating zf1::yfp knock-ins using unc-119 as a marker

    Depositor

    Jeremy Nance

    Article

    Armenti et al Development. 2014 Dec;141(23):4640-7. do

    Inserts

    ZF1 domain from pie-1
    N-terminal half of yfp with introns
    intron containing floxed unc-119 gene in reverse orientation
    C-terminal half of yfp with introns
    

    Expression

    Worm

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. sox10:PDGFRA wild-type

    Plasmid

    #98696

    Purpose

    I-SceI meganuclease-mediated human PDGFRA wild-type transgenesis in zebrafish

    Depositor

    Thomas Look

    Article

    Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

    Insert

    PDGFRA (PDGFRA Human)
    

    Use

    Zebrafish expression

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEPQD1CB0107

    Plasmid

    #177040

    Purpose

    MoClo Level 1, position 1, transcriptional unit for transient expression of 1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEPQD1CB0202

    Plasmid

    #185626

    Purpose

    MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    SpCas9
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBAD-SCW-HHL

    Plasmid

    #190627

    Purpose

    Bacterial expression of genetically encoded calcium indicator SCW-HHL

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCW-HHL
    

    Expression

    Bacterial

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBAD-SPW-HHL

    Plasmid

    #190629

    Purpose

    Bacterial expression of genetically encoded protease indicator SPW-HHL

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SPW-HHL
    

    Expression

    Bacterial

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pVRa28_1088

    Plasmid

    #49683

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF28_1088
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available Since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mCardinal-MannII-N-10

    Plasmid

    #56167

    Purpose

    Localization: Mannosidase/Golgi, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    MannII
    

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    aa 1-112 of MannII

    Promoter

    CMV

    Available Since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

31. pcDNA-Flag-Trib1

    Plasmid

    #131156

    Purpose

    Mammalian expression of flag-tagged mouse TRIB1

    Depositor

    Karen Keeshan, Warren Pear

    Article

    Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 1

    Insert

    Trib1 (Trib1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. myc-Miro1 (lacking TM)

    Plasmid

    #127613

    Purpose

    Human Miro1 with the transmembrane deleted

    Depositor

    Josef Kittler

    Article

    Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

    Insert

    Miro1 (RHOT1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Deletion of transmembrane domain

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. sox10:PDGFRA mutant

    Plasmid

    #98697

    Purpose

    I-SceI meganuclease-mediated human PDGFRA mutant transgenesis in zebrafish

    Depositor

    Thomas Look

    Article

    Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

    Insert

    PDGFRA (PDGFRA Human)
    

    Use

    Zebrafish expression

    Mutation

    exon 8 and 9 deletion

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pPB-CAG-mNanog-mut-pgk-hph

    Plasmid

    #74914

    Purpose

    Mammalian expression of mNanog (Y42E, K43T)

    Depositor

    Jose Silva

    Article

    Theunissen et al Development. 2011 Nov;138(22):4853-65. d

    Insert

    Nanog (Nanog Mouse)
    

    Expression

    Mammalian

    Mutation

    K43T of Nanog protein and Y42E of Nanog homeodoma…

    Promoter

    CAG

    Available Since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBabe_puro_DEST_Flag_TLOC1 (220aa)

    Plasmid

    #45273

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    TLOC1 (SEC62 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

36. pLJM1-SLC35B2-FLAG

    Plasmid

    #154863

    Purpose

    Transient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 (SLC35B2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    ctcctggtgcagtacttc => ttattagtccaatatttt

    Promoter

    CMV

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PLK3 1-334 T219D-pEGFP

    Plasmid

    #23268

    Depositor

    Wei Dai

    Article

    Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

    Insert

    PLK3 (PLK3 Human)
    

    Tags

    pEGFP

    Expression

    Mammalian

    Mutation

    aa 1-334 T219D

    Available Since

    Dec. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

38. pLJC6-Flag CASTOR1 I280A

    Plasmid

    #84497

    Purpose

    Lentiviral expression of CASTOR2 I280A mutant

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR1 I280A (GATSL3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    I280A

    Promoter

    sestrin2

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

39. pWL1 (mANGPTL8-V5-His)

    Plasmid

    #109113

    Purpose

    Expresses V5 and His-tagged mouse ANGPTL8 in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

    Insert

    ANGPTL8 (Angptl8 Mouse)
    

    Tags

    6x His and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 21, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. PD-L1-miSFIT-6-C, 16-C

    Plasmid

    #124689

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-L1 (CD274 Human)
    

    Expression

    Mammalian

    Promoter

    minimal CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge