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beta-arrestin1 mYFP

Plasmid

#36916

Depositor

Robert Lefkowitz

Article

Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

Insert

beta-arrestin1 (Arrb1 Rat)

Tags

mYFP

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

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pGY577

Plasmid

#166663

Purpose

Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination

Depositor

Gaël Yvert

Article

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

Cre: E340A, D341A

Promoter

CMV

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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TFORF2244

Plasmid

#143182

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TCF15 (TCF15 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMOS028: Mitycam calcium sensor (mitochondrial)

Plasmid

#163046

Purpose

Mitycam calcium sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Mitycam

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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ETV7_pet28a

Plasmid

#131656

Purpose

Bacterial expression of ETS gene ETV7

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV7 (ETV7 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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TFORF2978

Plasmid

#144454

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NR0B2 (NR0B2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN-puro-P2A-FRB-Fis1

Plasmid

#135297

Purpose

stably express FBR-Fis1 in mammalian cells with puromycin selection

Depositor

Richard Youle

Article

Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

Insert

FRB-Fis1 (FIS1 Human)

Use

Retroviral

Tags

FRB

Expression

Mammalian

Mutation

Fis1 tail (aa 93-152)

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-OMP25

Plasmid

#141150

Purpose

Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

OMP25 (Synj2bp Rat)

Tags

AcGFP

Expression

Mammalian

Mutation

OMP25 c terminus from addgene plasmid #69598

Promoter

CMV

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pRM27

Plasmid

#163695

Purpose

ubb promoter driven expression of lck:mNeonGreen

Depositor

David Matus

Article

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

lck:mNeonGreen (lck Zebrafish)

Use

Zebrafish expression

Tags

mNeonGreen

Promoter

ubb

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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EPS15-GFP-His

Plasmid

#170860

Purpose

Mammalian expression of EPS15-GFP-His.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Epidermal growth factor receptor pathway substrate 15 (EPS15) (EPS15 Human)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pRH2502

Plasmid

#84379

Purpose

Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

Depositor

Robert Husson

Article

Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

Insert

dcas9

Use

CRISPR

Expression

Bacterial

Mutation

Aspartate at position 10 changed to Alanine, Hist…

Promoter

uv15tetO

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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FUGW-PK-hLC3

Plasmid

#61460

Purpose

Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid)

Depositor

Isei Tanida

Article

Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

Insert

PK-hLC3 (MAP1LC3B Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pLR02_pXL001_HLAEB2M

Plasmid

#131087

Purpose

Overexpression of HLA-E fused to B2M

Depositor

Xiaojun Lian

Insert

HLA-E and B2M with flexible linker

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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WT TXNIP-FLAG

Plasmid

#200091

Purpose

WT human TXNIP with C-terminal 3xFLAG tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

TXNIP (TXNIP Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pKAR5

Plasmid

#149461

Purpose

Broad-Host range expression vector, RK2 and pUC origin, AraC activator/repressor, PBAD promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

AraC-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pYAL_HLA-A2_MART1

Plasmid

#171177

Purpose

Displays MART-1 peptide in HLA-A*02 as a single-chain trimer

Depositor

Michael Birnbaum

Article

Huisman et al Methods Mol Biol. 2022;2491:263-291. doi

Insert

MART1_HLA-A2*01 single-chain trimer fusion

Expression

Bacterial and Yeast

Promoter

GAL1

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET29b-rhMOG

Plasmid

#225484

Purpose

Express the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coli

Depositor

John Pak

Article

Wu et al J Immunol Methods. 2024 Oct 13:113764. d

Insert

Soluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)

Tags

6xHis tag

Expression

Bacterial

Mutation

deleted amino acids 1-29 (signal peptide) and 150…

Promoter

T7

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-SADB19P

Plasmid

#32631

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

SADB19P

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pBS CMV GAG

Plasmid

#234992

Purpose

For production of Viral like particles (VLPs) by expressing the MLV GAG protein in mammalian cells

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

GAG

Expression

Mammalian

Mutation

Deleted polymerase coding sequence

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1a-donor_opt(R2Tg)-GFP(intron)

Plasmid

#223252

Purpose

Mammalian expression of the optimized R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor_opt

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pIVM_02

Plasmid

#204500

Purpose

Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)

Depositor

Alessio Ciulli

Article

Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

Insert

amino acids 54-213 VHL (VHL Human)

Tags

6xHis, TEV, and Thrombin

Expression

Bacterial

Mutation

0

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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FB007

Plasmid

#119705

Purpose

Promoter gpdA from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

Promoter gpdA

Use

Synthetic Biology; Domestication of dna parts for…

Promoter

gpdA

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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Braf

Plasmid

#40775

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

Braf (BRAF Human)

Tags

FLAG

Expression

Mammalian

Mutation

Starting Met removed

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P08

Plasmid

#120019

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mClover3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mClover3

Use

Pcr template

Tags

mClover3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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U6-NT sgRNA; EF1a-dCas9-KRAB-GFP

Plasmid

#194284

Purpose

EF1a-dCas9-KRAB-GFP with nontarget sgRNA

Depositor

Elizabeth Kirby

Article

Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

Insert

dCas9-KRAB-GFP

Use

CRISPR and Lentiviral

Tags

GFP

Promoter

EF1a

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_LKB1

Plasmid

#108111

Purpose

Lentiviral expression plasmid of human LKB1 cDNA with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

LKB1 (STK11 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS promoter

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKS042

Plasmid

#65470

Purpose

pTrc plasmid expressing clpA

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

clpA (clpA )

Use

Synthetic Biology

Expression

Bacterial

Promoter

trc

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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AiP15263 - pAAV-AiE0219h-minBG-iCre(R297T)-BGHpA (Alias: CN5263)

Plasmid

#230406

Purpose

AiE0219h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pC0037

Plasmid

#181934

Purpose

Dual secreted luciferase reporter (EF1alpha-Gaussia luciferase, CMV-Cypridina luciferase)

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Inserts

Gaussia luciferase
Cypridina luciferase

Expression

Mammalian

Promoter

CMV and EF1alpha

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMiniWhiteA2

Plasmid

#165873

Purpose

Basic transgenesis Mini-white physical eye marker cassette in Alpha2, lacks attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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