We narrowed to 7,473 results for: addgene/1000

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pLJM1-FH-AGO2-5xE

Plasmid

#91981

Purpose

Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S828E, T830E, S831E, S834E (5xE)

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-JUN

Plasmid

#192895

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of JUN

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

JUN (JUN Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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CAG-VEGF120-T2A-mCherry

Plasmid

#229134

Purpose

Retrovirus driving overexpression of VEGF120 plus mCherry reporter

Depositor

Elizabeth Kirby

Article

Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

Inserts

VEGF120 (Vegfa Mouse)
mCherry

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ETS1(12))-PGKpuro2ABFP-W

Plasmid

#208550

Purpose

Lentiviral vector expressing gRNA targeting human ETS1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

ETS1(12) (ETS1 Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ETS1(11))-PGKpuro2ABFP-W

Plasmid

#208549

Purpose

Lentiviral vector expressing gRNA targeting human ETS1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

ETS1(11) (ETS1 Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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SITS-MmCav1-APEX2

Plasmid

#170526

Purpose

Vector expression of MmCav1-APEX2 in Leishmania cell free system. Parton lab clone IYW

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Caveolin1 (Cav1 Mouse)

Use

Leishmania cell free expression clone

Tags

APEX2 and Lifeact

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-ERK2-L4A-MEK1_fusion

Plasmid

#39197

Depositor

Melanie Cobb

Article

Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

Insert

ERK2-MEK1 Fusion (MAP2K1, Mapk1 Human, Rat)

Tags

Myc

Expression

Mammalian

Mutation

L4A = L33A, L37A, L40A, L42A

Promoter

CMV

Available Since

July 25, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR2b-ITGB1(WT)-mRuby2

Plasmid

#215446

Purpose

Gateway entry vector with integrin beta1 tagged with mRuby2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway entry vector

Tags

mRuby2

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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hsp70l: hAPOB48-mCherry.

Plasmid

#140639

Purpose

Heat shock promoter driven expression of human APOB48-mCherry. The construct has tol2 elements, and is designed to use in zebrafish.

Depositor

Steven Farber

Article

Walsh et al J Lipid Res. 2019 Dec 30. pii: jlr.RA119

Inserts

hsp70l (hsp70l Zebrafish)
APOB (APOB Human)

Use

Zebrafish expression

Tags

mCherry

Mutation

APOB48: amino acid 1-2180

Promoter

hsp70l

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-nuc-GsCT

Plasmid

#205517

Purpose

Nuclear-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

nuc-GsCT (GNAS Synthetic, Human)

Tags

Histone H2A and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-SynI-CreOn-FlpOff-Kir2.1-P2A-EGFP

Plasmid

#176278

Purpose

Viral vector for co-expression of Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Article

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

Kir2.1-P2A-EGFP (Kcnj2 Synthetic, Mouse)

Use

AAV and Cre/Lox

Tags

Myc

Expression

Mammalian

Promoter

human Synapsin I

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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FLT1-KDR

Plasmid

#86034

Purpose

chimera of human VEGFR1 extracellular domain with the TMD fused to the intracellular domain of VEGFR2

Depositor

Christie Thomas

Article

Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.

Insert

human VEGFR1-VEGFR2 hybrid (FLT1, KDR Human)

Tags

HA and t-GFP

Expression

Mammalian

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with interspersed loops (GAPDH)

Plasmid

#180183

Purpose

AAV vector carrying a guide RNA targeting the human GAPDH mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with interspersed loops (GAPDH Human)

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pUCIDT-attL1-Human ABeta-attR5

Plasmid

#160436

Purpose

Entry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Article

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Homo sapiens amyloid beta precursor protein (APP Human)

Expression

Bacterial

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pODN-mR2-ACTB

Plasmid

#183868

Purpose

Repair template for the N-terminal tagging of beta-actin with mRuby2 in human cells using CRISPR/Cas9.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ACTB homology arms with mRuby2-linker (ACTB Human)

Use

CRISPR; Donor template

Tags

mRuby2-linker

Expression

Mammalian

Mutation

Homology arms contain point mutations to remove t…

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-ZIP(RK)

Plasmid

#27136

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Arginines in the SH2 domains mutated to lysines (…

Available Since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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pRRLHygro-pEF1a-p53ashL344P-mKate2-splitmVenusC

Plasmid

#69587

Purpose

Expresses mutated p53-L344P tagged with mKate2 and split C-term mVenus

Depositor

Galit Lahav

Article

Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

Insert

p53-L344P (TP53 Human)

Use

Lentiviral

Tags

mKate2 and split mVenus - C term

Expression

Mammalian

Mutation

p53 L344P mutation that prevents dimerization and…

Promoter

EF1alpha

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268

Plasmid

#69219

Purpose

Expresses wild type SpCas9 fused to Zif268 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

Zif268 (Egr1 Mouse)

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-ZIP(FF)

Plasmid

#27135

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Tyrosines in ITAMS 1-3 mutated to phenylalanines …

Available Since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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MLM3636-Prnp-CDS-Pos

Plasmid

#61857

Purpose

Expresses a gRNA plasmid targeting the prion gene's exon 3, positive strand

Depositor

Gerold Schmitt-Ulms

Article

Mehrabian et al PLoS One. 2014 Dec 9;9(12):e114594. doi:

Insert

PrP guiding sequence (Prnp Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

hU6 promoter

Available Since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only