We narrowed to 9,235 results for: CAG

1. pattB-LSL-AAVR-F2A-spCas9

   Plasmid

   #202459

   Purpose

   The plasmid backbone used to generate the recombination template to generate the SELECTIV mice through Integrase Mediated Transgenesis. Allows for Cre-dependent expression of AAVR and Cas9.

   Depositor

   Jan Carette

   Article

   Zengel et al Nat Methods. 2023 Jul;20(7):1070-1081. d

   Insert

   AAVR (AU040320 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   mCherry fusion

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR

   Plasmid

   #113162

   Purpose

   A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   SpCas9
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG

   Available Since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Bxb1-GT-Dox-AIO-TetOn_Ngn2-ISL1-LHX3_Down-Tandem

   Plasmid

   #241396

   Purpose

   Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of Ngn2, ISL1 and LHX3

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-Ngn2-ISL1-LHX3; rtTA-T2A-mTagBFP2 (ISL1, LHX3, NEUROG2 Human)
   

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   TRE; CAG

   Available Since

   Oct. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Bxb1-GA_AIO-GZV-SynZiFTR_ETV2_Down-Tandem

   Plasmid

   #241394

   Purpose

   Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression of ETV2

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   ETV2; ZF10-NS3-p65 (ETV2, RELA, KRAS Human)
   

   Expression

   Mammalian

   Promoter

   ZF10; CAG

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTwist-SARS-CoV-2 Spike-deltaC18 B.1.617v5 (B.1.617.3)

   Plasmid

   #212540

   Purpose

   Encodes SARS-CoV-2 spike T19R,G142D,E156-F157 deletion,R158G,N450R,E484Q,D614G,P681R,D950N for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike T19R,G142D,E156-F157 deletion,R158G,N450R,E484Q,D614G,P681R,D950N (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   T19R,G142D,E156-F157 deletion,R158G,N450R,E484Q,D…

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTwist-SARS-CoV-2 Spike-deltaC18 BA.4

   Plasmid

   #212519

   Purpose

   Encodes SARS-CoV-2 variant BA.4 Spike for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike BA.4 (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   T19I ,deltaL24P25P26 ,A27S ,delH69-V70 ,G142D ,V2…

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTwist-SARS-CoV-2 Spike-deltaC18 Q493R (CGA) G496S (TCA) Q498R (CGA) N501Y (TAT) Y505H (CAT)

   Plasmid

   #212477

   Purpose

   Encodes SARS-CoV-2 spike Q493R, G496S, Q498R, N501Y, Y505H for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike Q493R, G496S, Q498R, N501Y, Y505H (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   Q493R, G496S, Q498R, N501Y, Y505H

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTwist-SARS-CoV-2 Spike-deltaC18 AY.1

   Plasmid

   #212433

   Purpose

   Encodes SARS-CoV-2 spike T19R ,T95I ,G142D ,deltaE156-F157+R158G ,W258L ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike T19R ,T95I ,G142D ,deltaE156-F157+R158G ,W258L ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   T19R ,T95I ,G142D ,deltaE156-F157+R158G ,W258L ,K…

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTwist-SARS-CoV-2 Spike-deltaC18 AY.2

   Plasmid

   #212434

   Purpose

   Encodes SARS-CoV-2 spike T19R ,V70F ,G142D ,deltaE156-F157+R158G ,A222V ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike T19R ,V70F ,G142D ,deltaE156-F157+R158G ,A222V ,K417N ,L452R ,T478K ,D614G ,P681R ,D950N (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   T19R ,V70F ,G142D ,deltaE156-F157+R158G ,A222V ,K…

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTwist-SARS-CoV-2 Spike-deltaC18 B.1.617v2 (B.1.617.1 v2)

    Plasmid

    #212429

    Purpose

    Encodes SARS-CoV-2 spike T95I,G142D,E154K,E156-F157 deletion,R158G,L452R,E484Q,D614G,P681R,Q1071H for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike T95I,G142D,E154K,E156-F157 deletion,R158G,L452R,E484Q,D614G,P681R,Q1071H (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    T95I,G142D,E154K,E156-F157 deletion,R158G,L452R,E…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTwist-SARS-CoV-2 Spike-deltaC18 SAK006879 VOC-501Y.V2 (B.1.351v5)

    Plasmid

    #212391

    Purpose

    Encodes SARS-CoV-2 spike D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,A701V for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,A701V (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTwist-SARS-CoV-2 Spike-deltaC18 SAK007134 VOC-501Y.V2 (B.1.351v6)

    Plasmid

    #212393

    Purpose

    Encodes SARS-CoV-2 spike A27S,D80A,D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,A701V for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike A27S,D80A,D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,A701V (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    A27S,D80A,D215G,L242-L244 deletion,K417N,E484K,N5…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTwist-SARS-CoV-2 Spike-deltaC18 (P.1) - No RBD Mutations

    Plasmid

    #212384

    Purpose

    Encodes SARS-CoV-2 spike L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V1176F for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V1176F (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V11…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTwist-SARS-CoV-2 Spike-deltaC18 SA190 VOC-501Y.V2 (B.1.351v4)

    Plasmid

    #212389

    Purpose

    Encodes SARS-CoV-2 spike D80A,D215G,L242-L244 deletion,E484K,N501Y,D614G,A701V for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike D80A,D215G,L242-L244 deletion,E484K,N501Y,D614G,A701V (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    D80A,D215G,L242-L244 deletion,E484K,N501Y,D614G,A…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTwist-SARS-CoV-2 Spike-deltaC18 VOC-501Y.V2 (B.1.351) - All Mutations

    Plasmid

    #212381

    Purpose

    Encodes SARS-CoV-2 spike L18F,D80A,D215G,L242H,A243I,Q414N,E484K,N501Y,D614G,A701V for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike L18F,D80A,D215G,L242H,A243I,Q414N,E484K,N501Y,D614G,A701V (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    L18F,D80A,D215G,L242H,A243I,Q414N,E484K,N501Y,D61…

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCXLE-mCherry-t2a-SOX2AV-p2a-KLF4

    Plasmid

    #193295

    Purpose

    episomal vector expressing mCherry + human SOX2A61V + KLF4

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry-t2a-SOX2AV-p2a-KLF4 (SOX2, KLF4 Human, Discosoma)
    

    Expression

    Mammalian

    Mutation

    SOX2A61V is a highly cooperative point mutant of …

    Promoter

    CAG

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-gRNA(SFTPCI73Tcorr)-SpCas9(BB)-2A-GFP

    Plasmid

    #187647

    Purpose

    Encodes gRNA to correct the SFTPC I73T mutation

    Depositor

    Darrell Kotton

    Article

    Alysandratos et al Cell Rep. 2021 Aug 31;36(9):109636. doi:

    Insert

    I73T gRNA
    

    Use

    CRISPR

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUCM-CLYBL-NGN2-BRN3A

    Plasmid

    #165597

    Purpose

    Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.

    Depositor

    Carsten Bönnemann

    Article

    Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    BRN3A (POU4F1 Human)
    EGFP
    Puromycin N-acetyl transferase
    rtTA3G
    SBP Delta-LNGFR
    

    Use

    CRISPR and TALEN

    Expression

    Mammalian

    Promoter

    CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…

    Available Since

    March 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCXLE-SOX2-17-p2a-KLF4

    Plasmid

    #193290

    Purpose

    episomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversion

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    SOX2-17-p2a-KLF4 (SOX2, KLF4 Human)
    

    Expression

    Mammalian

    Mutation

    SOX2-17 is engineered highly cooperative chimeric…

    Promoter

    CAG

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4

    Plasmid

    #193294

    Purpose

    episomal mCherry-hS*K (expressing mCherry + human super-SOX + KLF4) for integration-free naïve conversion

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry-t2a-SOX2-17-p2a-KLF4 (SOX2, KLF4 Human, Discosoma)
    

    Expression

    Mammalian

    Mutation

    SOX2-17 is engineered highly cooperative chimeric…

    Promoter

    CAG

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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