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We narrowed to 5,502 results for: IRES

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Showing: 5481 - 5500 of 5502 results

  1. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid
    #223137
    Purpose
    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223137-plasmid-map-sequence-id-447707

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  3. pJC2528 - CLYBL-Neo-CAGGS-LSL-dCas9-TagBFP-KRAB-CLYBL (LSL-CRISPRi)

    Plasmid
    #250253
    Purpose
    Homology donor for knock-in of CRISPRi system into CLYBL locus with addgene plasmids 62196 and 62197. Lox-Stop-Lox requires cre delivery for activation.
    Depositor
    Jacob Corn
    Article
    Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:
    Insert
    dCas9-mTagBFP-KRAB
    Use
    CRISPR
    Tags
    HA-2xSV40 NLS-mTagBFP-KRAB
    Expression
    Mammalian
    Promoter
    CAGGS
    Available Since
    Jan. 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    250253-plasmid-map-sequence-id-498726

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  4. EGFP-p300 (D1399Y)

    Plasmid
    #191763
    Purpose
    Expression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)
    Depositor
    Daniel Gerlich
    Article
    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:
    Insert
    FRB-EGFP-p300 core (D1399Y) (EP300 Human)
    Tags
    EGFP (N-terminal of p300 core (D1399Y)) and FRB (…
    Expression
    Bacterial and Mammalian
    Mutation
    catalytic D1399Y mutation in p300
    Promoter
    EF1alpha
    Available Since
    Feb. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    191763-plasmid-map-sequence-id-383798

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  8. str-KDEL_Stx5LdeltaER-SBP-mCitrine

    Plasmid
    #154846
    Purpose
    Synchronize trafficking of Stx5LdeltaER (M55V) from the ER, for use with FLIM (RUSH system)
    Depositor
    Geert van den Bogaart
    Article
    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:
    Insert
    Stx5 (Long isoform) (STX5 Human)
    Tags
    Streptavidin Binding Protein (SBP) and mCitrine
    Expression
    Mammalian
    Mutation
    Changed Methionine 55 to Valine, causes loss of S…
    Promoter
    CMV
    Available Since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    154846-plasmid-map-sequence-id-348286

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  9. str-KDEL_Stx5L(M55V)-SBP-mCitrine

    Plasmid
    #209846
    Purpose
    Synchronize trafficking of Stx5L (M55V) from the ER, for use with FLIM (RUSH system)
    Depositor
    Geert van den Bogaart
    Article
    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:
    Insert
    STX5 (STX5 Human)
    Tags
    Streptavidin Binding Protein (SBP) and mCitrine
    Expression
    Mammalian
    Mutation
    Changed Methionine 55 to Valine, causes loss of S…
    Promoter
    CMV
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    209846-plasmid-map-sequence-id-413647

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  10. str-KDEL_Stx5S-SBP-mCitrine

    Plasmid
    #154845
    Purpose
    Synchronize trafficking of Stx5S from the ER, for use with FLIM (RUSH system)
    Depositor
    Geert van den Bogaart
    Article
    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:
    Insert
    Stx5 (Short isoform) (STX5 Human)
    Tags
    Streptavidin Binding Protein (SBP) and mCitrine
    Expression
    Mammalian
    Mutation
    Lacks amino acids 1-54, enocdes short isoform of …
    Promoter
    CMV
    Available Since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    154845-plasmid-map-sequence-id-325019

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  16. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

    Plasmid
    #52093
    Purpose
    Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine
    Depositor
    Dario Vignali
    Article
    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:
    Inserts
    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    Use
    Retroviral
    Tags
    F2A, IRES-EGFP, P2A, and T2A
    Expression
    Mammalian
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52093-plasmid-map-sequence-id-80507

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  17. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

    Plasmid
    #52092
    Purpose
    Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP
    Depositor
    Dario Vignali
    Article
    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:
    Inserts
    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    Use
    Retroviral
    Tags
    F2A, IRES-EGFP, P2A, and T2A
    Expression
    Mammalian
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52092-plasmid-map-sequence-id-80506

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Showing: 5481 - 5500 of 5502 results