19,654 results

1. pZT-AAVS1-L1

   Plasmid

   #52637

   Purpose

   TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

   Depositor

   Mahendra Rao, Jizhong Zou

   Article

   Luo et al Stem Cells Transl Med. 2014 May 15. pii:

   Insert

   Left AAVS1 TALEN
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET51b-His-TEV-HaloTag7

   Plasmid

   #167266

   Purpose

   Overexpression of HaloTag7 in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   HaloTag7
   

   Tags

   His x10

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLIX403_UBAP2L_mCherry

   Plasmid

   #105286

   Purpose

   puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein

   Depositor

   Eugene Yeo

   Article

   Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

   Insert

   UBAP2L (UBAP2L Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin

   Plasmid

   #134858

   Purpose

   Expresses EGFP-tagged early endosome marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Ras-related protein 5A
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-GFP SNAP25

   Plasmid

   #170872

   Purpose

   Mammalian expression of His-GFP SNAP25.

   Depositor

   Justin Taraska

   Article

   Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

   Insert

   SNAP 25 (Snap25 Rat)
   

   Tags

   (His)6 and EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. ratGlp1-R

   Plasmid

   #14944

   Depositor

   Bernard Thorens

   Article

   Thorens et al Proc Natl Acad Sci U S A. 1992 Sep 15. 8

   Insert

   Glp1-R (Glp1r Rat)
   

   Expression

   Mammalian

   Available Since

   June 7, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG

   Plasmid

   #155305

   Purpose

   Lentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.

   Depositor

   Stanley Qi

   Article

   Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

   Insert

   mCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
   

   Use

   Lentiviral

   Tags

   2xNLS-3xFLAG

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSL1119 (pDonor)

   Plasmid

   #160732

   Purpose

   Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST donor DNA (Mini-Tn)
   

   Expression

   Bacterial

   Promoter

   N/A

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40

   Plasmid

   #100853

   Purpose

   AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV Retrograde and AAV1

   Insert

   jRGECO1a
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCB-hDot1Lwt

    Plasmid

    #74173

    Purpose

    For packaging hDot1Lwt to retrovirus.

    Depositor

    Yi Zhang

    Article

    Okada et al Cell. 2005 Apr 22;121(2):167-78.

    Insert

    hDot1L (DOT1L Human)
    

    Use

    Retroviral

    Tags

    HA

    Available Since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1227 pcDNA3 Myr HA Akt2

    Plasmid

    #9016

    Depositor

    William Sellers

    Insert

    Akt2 (AKT2 Human)
    

    Tags

    HA and Myr

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-HIF2alpha-P405A/P531A-pBabe-puro

    Plasmid

    #19006

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: P405A and P531A

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXs-IP-mVenus-TOSI

    Plasmid

    #172491

    Purpose

    mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

    Depositor

    David Scadden

    Article

    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

    Insert

    Pdcd4 (Pdcd4 Mouse)
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Promoter

    MLV LTR

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Myc-NLRP3(R260W)

    Plasmid

    #73956

    Purpose

    Express c-Myc-tagged human NLRP3(R260W) in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Dorfleutner et al Infect Immun. 2007 Mar;75(3):1484-92. Ep

    Insert

    NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
    

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    R260W

    Promoter

    CMV iE

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

15. Ai66(RCRL-tdT) targeting vector

    Plasmid

    #61578

    Purpose

    Target a Dre and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    CAG-RSR-LSL-tdTomato
    

    Use

    Cre/Lox and Mouse Targeting

    Promoter

    CAG

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-Neo-Bam p53 R273H

    Plasmid

    #16439

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R273H (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    R273H

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CW3SL-EGFP

    Plasmid

    #61463

    Purpose

    AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

    Insert

    EGFP
    

    Use

    AAV

    Promoter

    CaMKIIa

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cys-60nmSAH-SPYcatcher

    Plasmid

    #214840

    Purpose

    This plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

    Insert

    Cys-60nmSAH-SPYcatcher
    

    Tags

    His-TEV-SUMO-

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3 p53 WT

    Plasmid

    #69003

    Purpose

    mammalian expression of wild type human p53

    Depositor

    David Meek

    Article

    Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

    Insert

    p53 (TP53 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. BcatMutS33/S37.T41/S45

    Plasmid

    #24204

    Depositor

    David Rimm

    Insert

    beta catenin (CTNNB1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S33A, S37A, T41A,S45A

    Promoter

    CMV

    Available Since

    March 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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