We narrowed to 16,501 results for: form
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Plasmid#169723PurposeMammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, I217K, Y353E)DepositorInsertSPOP (Spop Mouse)
TagsV5ExpressionMammalianMutationL186D, L190D, L193D, I217K, Y353EPromoterCMVAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-hRNF216
Plasmid#167354PurposeExpression of human RNF216 (Triad3A) codon optimized for E. coli and insect cellsDepositorInsertE3 ubiquitin-protein ligase RNF216 (RNF216 Human)
Tags3C protease cleavage site and 6x-His tagExpressionBacterialMutationcodon optimised for expression in E. coliPromoterT7Available SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSYN-DRD2-V2tail-TevN-BLITz1-TetR-VP16-bGHpA
Plasmid#89874Purposeexpresses DRD2-iTango component in mammalian cells for AAV productionDepositorInsertDRD2-V2tail-TevN-BLITz1-TetR-VP16
UseAAVTagsFLAGExpressionMammalianMutationshort isoform of DRD2 (deletion of 242-270)PromoterHuman synapsinAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a
Plasmid#40032DepositorAvailable SinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBW2436_pCAG-PV1-GAI-L1-Cre-271C-NLS-BGHpA
Plasmid#108730PurposeExpresses aa271 to C-terminus of Cre recombinase fused to GAI CIDDepositorInsertCre recombinase fragment aa271 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-n1-AICD
Plasmid#69925Purposemammalian expression of human AICD fused to EGFPDepositorAvailable SinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
VP-FKBP51
Plasmid#89097Purposemammalian expression of FKBP5 also referred to as FKBP51 with N-terminal VP16 activation domainDepositorAvailable SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-Junb
Plasmid#178441Purposedoxycycline-inducible expression of Mouse Junb in mammalian cellsDepositorAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Topo_SpCas9.tracr_AsCas12a.DR
Plasmid#155050PurposeTOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vectorDepositorInsertSpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
UseCRISPRAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPCX2-HA-GATA3 FL
Plasmid#89835PurposeExpress GATA3 in mammalian cellsDepositorAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
5HT2C-RNA2-Flag
Plasmid#79679Purposeserotonin receptor 2C isoform 2 with C-terminal Flag tag, non-edited isoformDepositorAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-TCF7
Plasmid#79498PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human TCF7 CDSDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
PD2529 human alpha5 full
Plasmid#221395PurposeMammalian expression of human integrin alpha5 full lengthDepositorInsertintegrin alpha5 full length (ITGA5 Human)
TagsCD33 secretion peptide (MPLLLLLPLLWAGALA)ExpressionMammalianAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFR D837A
Plasmid#11014PurposeRetroviral construct for expressing human EGFR with D837A mutation in human cellsDepositorAvailable SinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CoV-2_linker
Plasmid#211731PurposeLinker fragment for direct rescue of SARS-CoV-2, including 5' Promoter and 3' Termination signals and 100bp SARS-CoV-2 homology regionsDepositorInsertLinker fragment SARS-CoV-2
ExpressionMammalianAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAaePUb::Cas9-2A-Neo
Plasmid#176680PurposeExpression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)DepositorInsertspCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
UseCrisprExpressionInsectMutationDrosophila codon optimizedPromoterAe. aegypti poly-ubiquitin (AAEL003888)Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP
Plasmid#35501PurposeAAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.DepositorInserthChR2
UseAAVTagsEYFPExpressionMammalianMutationC128S and D156APromoterCaMKIIaAvailable SinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3−3xFLAG−AGO2(D669A)
Plasmid#220461PurposeExpress catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cellsDepositorInsertHuman AGO2 (AGO2 Human)
Tags3xFLAGExpressionMammalianMutationAspartate 669 to alaninePromoterCMVAvailable SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBW2176_pCAG-PV1-GAI-L1-FlpO-28C-NLS-BGHpA
Plasmid#108748PurposeExpresses aa28 to C-terminus of Flp recombinase fused to GAI CIDDepositorInsertFlp recombinase fragment aa28 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-tdTomato-FlagAkt1W80A
Plasmid#64833PurposecDNA of Akt1 with point mutation from Trp to Ala at position 80. This mutation confers resistance to the MK-2206 drugDepositorInsertAkt1 (Akt1 Mouse)
UseLentiviralTags3xFlagExpressionMammalianMutationPoint mutation from Trp to Ala at position 80PromoterCMVAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcsII-MECL1-flag
Plasmid#86774PurposeC-terminal flag-tagged protein expression in mammalian cells, lentiviral vectorDepositorInsertimmunoproteasome beta subunit 2 (MECL1) (PSMB10 Human)
UseLentiviralTagsflagExpressionMammalianPromoterunknownAvailable SinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23_Promoter_1-3
Plasmid#210629PurposeLuciferase experiment for TAL1 promoter 1-3DepositorAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
5HT2C-RNA1-FLAG
Plasmid#79678Purposeserotonin receptor 2C RNA isoform 1 with C-terminal flag tagDepositorAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_HPRT1_exon2_1_Lb
Plasmid#155051PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of HPRT1 exon 2
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW2209_pCAG-PV1-iCre-N229-L1-GID1-NLS-BGHpA
Plasmid#108723PurposeExpresses N-terminus to aa229 of Cre recombinase fused to GID1 CIDDepositorInsertCre recombinase fragment N-terminus to aa229
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2229_pCAG-PV1-GAI-L1-iCre-230C-NLS-BGHpA
Plasmid#108724PurposeExpresses aa230 to C-terminus of Cre recombinase fused to GAI CIDDepositorInsertCre recombinase fragment aa230 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_HPRT1_exon3_1_Lb
Plasmid#155053PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of HPRT1 exon 3
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6A NLRP1 CARD-FLAG WT
Plasmid#163978PurposeMammalian expression vector encoding human NLRP1 CARD with a C-terminal FLAG tagDepositorAvailable SinceJan. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DHX36iso2
Plasmid#159586PurposeExpress N-ter FLAG HA tagged DHX36 isoform 2 in mammalian cellsDepositorAvailable SinceSept. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23_Promoter_4
Plasmid#210630PurposeLuciferase experiment for TAL1 promoter 4DepositorAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only