2,225 results

1. pLKO.1 mTagBFP2 rat PGK1 shRNA

   Plasmid

   #222869

   Purpose

   Expresses mTagBFP2 along with an shRNA against rat PGK1

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   PGK1 shRNA (Pgk1, Pgk1 Mouse, Rat)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBactin-Nedd1-mOrange2-hCM1

   Plasmid

   #196863

   Purpose

   Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Nedd1-mOrange2-hCM1 (Nedd1 Human, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-Ef1a-DIO_EGFP-RandE3

   Plasmid

   #79872

   Purpose

   For generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-RandE3
   

   Use

   AAV, Cre/Lox, and Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLD003-pCMV-APOBEC-Cas9(D10A)-rPB(8kD)

   Plasmid

   #165445

   Purpose

   Expresses ACX, rPB(8kD) in mammalian cells

   Depositor

   Wei Leong Chew

   Article

   Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

   Insert

   APOBEC-nCas9-rPB(8kD)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-AU1-mTOR-L1460P

   Plasmid

   #19995

   Depositor

   Fuyuhiko Tamanoi

   Article

   Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

   Insert

   mammalian target of rapamycin-L1460P (Mtor Rat)
   

   Use

   Tags

   AU1

   Expression

   Mammalian

   Mutation

   Changed Leucine 1460 to Proline

   Promoter

   Available since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

6. miniSOG-RatLC3

   Plasmid

   #57775

   Purpose

   Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   LC3
   

   Use

   Tags

   miniSOG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. CC10-rtTA

   Plasmid

   #20179

   Depositor

   Jeffrey Whitsett

   Article

   Stripp et al J Biol Chem. 1992 Jul 25. 267(21):14703-

   Insert

   rcc10 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2010

   Availability

   Academic Institutions and Nonprofits only

8. Cherry-HA-KCC2

   Plasmid

   #104077

   Purpose

   To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

   Depositor

   Igor Medyna

   Article

   Kahle et al EMBO Rep. 2014 Jul;15(7):766-74. doi: 10

   Insert

   KCC2 (b isoform) (Slc12a5 Rat)
   

   Use

   Tags

   mCherry-HA tag

   Expression

   Mammalian

   Mutation

   EcoR1 site in rat KCC2 was removed by silent muta…

   Promoter

   UbC

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOC1 Halo-Actb KI

   Plasmid

   #183426

   Purpose

   CreON knock-in for Halo-beta-actin (amino acid position: before start codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and Halo donor
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

    Plasmid

    #118273

    Purpose

    pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

    Depositor

    Naoshige Uchida

    Article

    Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis (N terminal on insert), T7 epitope, and Xpr…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mGlurR5a/pCI-Neo

    Plasmid

    #197305

    Purpose

    Plasmid expressing an antigen targeting the mGluR1/5 metabotropic glutamate receptor

    Depositor

    Robert Gereau

    Insert

    mGluR5a (Grm5 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EMSV-myogenin (puro)

    Plasmid

    #14712

    Depositor

    Michael Rudnicki

    Article

    Sabourin et al Clin Genet. 2000 Jan . 57(1):16-25.

    Insert

    myogenin (Myog Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RnBarr2-mVenus

    Plasmid

    #137793

    Purpose

    Visualization of beta-arrestin2

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin2 (Arrb2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBa-FRB-3myc-KIF1A tail 391-1698

    Plasmid

    #64286

    Purpose

    KIF1A aa 391-1698 with N-terminal FRB and triple myc tags

    Depositor

    Marvin Bentley, Gary Banker

    Article

    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

    Insert

    KIF1A (Kif1a, Kif1a Mouse, Rat)
    

    Use

    Tags

    3myc and FRB

    Expression

    Mammalian

    Mutation

    aa 391-1698, 5'end mostly matches rat, 3…

    Promoter

    chicken Beta Actin

    Available since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. G protein alpha-o-YFP

    Plasmid

    #55776

    Purpose

    This G protein alpha-o construct contains internal insertions of YFP and the EE epitope

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    G protein alpha-o internally tagged with EYFP and EE epitope (Gnao1 Rat)
    

    Use

    Tags

    EE epitope was introduced internally with D167E a…

    Expression

    Mammalian

    Mutation

    A BglII site was removed from the 5' untrans…

    Promoter

    CMV

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCI pHluorin Syt4

    Plasmid

    #184505

    Purpose

    Mammalian expression of Synaptotagmin 4 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 4 (Syt4 Rat)
    

    Use

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB-Gata2

    Plasmid

    #60665

    Purpose

    PiggyBac expression vector encoding rat Gata2 downstream of a doxycycline inducible promoter

    Depositor

    James Thomson

    Article

    Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

    Insert

    Gata2 (Gata2 Rat)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRc/CMV HA TrkA

    Plasmid

    #39977

    Depositor

    Yves-Alain Barde

    Article

    Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

    Insert

    TrkA (Ntrk1 Rat)
    

    Use

    Tags

    BM40 signal sequence and HA

    Expression

    Mammalian

    Mutation

    endogenous signal sequence was replaced with BM40…

    Promoter

    CMV

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. G protein alpha-s(alpha3beta5/G226A)

    Plasmid

    #55798

    Purpose

    In addition to the mutations in alpha-s(alpha3beta5), this dominant negative G protein alpha-s mutant contains the G226A mutation, which impairs activating conformation changes in Switch II.

    Depositor

    Catherine Berlot

    Article

    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

    Insert

    alpha-s (alpha3beta5/G226A) (Gnas Rat)
    

    Use

    Tags

    internal EE epitope (residues 189-194 in alpha-s …

    Expression

    Mammalian

    Mutation

    N271K, K274D, R280K, T284D, I285T, G226A in alpha…

    Promoter

    CMV

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YPet-Lysosomes-20

    Plasmid

    #56636

    Purpose

    Localization: Lysosome Membrane, Excitation: 513, Emission: 527

    Depositor

    Michael Davidson

    Insert

    LAMP1
    

    Use

    Tags

    YPet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

21. pMT ERK1

    Plasmid

    #12656

    Depositor

    John Kyriakis

    Insert

    ERK1 (Mapk3 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2006

    Availability

    Academic Institutions and Nonprofits only

22. PKC zeta WT

    Plasmid

    #21248

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC zeta (Prkcz Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

23. VAMP2-SNAPtag

    Plasmid

    #107372

    Purpose

    Synaptic vesicle protein VAMP2 fused to the SNAPtag domain

    Depositor

    David Perrais

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Tags

    SNAPtag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGFP-p97-E578Q

    Plasmid

    #85672

    Purpose

    Expression of human p97 E578Q mutant with N-terminal GFP tag

    Depositor

    Hemmo Meyer

    Article

    Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

    Insert

    p97 (Vcp Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    E578Q

    Promoter

    CMV

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. α1GA-HE3-pcDNA3

    Plasmid

    #45812

    Depositor

    Edward Perez-Reyes

    Insert

    α1Ga (Cacna1g Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMK277 (mClover-NeoR)

    Plasmid

    #72793

    Purpose

    C-terminal tagging with mClover using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mClover-Neo
    

    Use

    CRISPR

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FKBP-AP180-GFP

    Plasmid

    #186577

    Purpose

    Expression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896.

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    AP180 (Snap91 Rat)
    

    Use

    Tags

    FKBP N-terminal GFP C-terminal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA HisB TO rFez1

    Plasmid

    #17385

    Depositor

    Stephen Goff

    Article

    Naghavi et al Genes Dev. 2005 May 1. 19(9):1105-15.

    Insert

    FEZ1 (Fez1 Rat)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2010

    Availability

    Academic Institutions and Nonprofits only

29. pscALPSpuro-RnACE2 (Rat)

    Plasmid

    #158086

    Purpose

    Expresses rat ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    Rat ACE2 (Ace2 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10

    Plasmid

    #218867

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8f
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    Q315L

    Promoter

    hsp70

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge