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Plasmid#13335DepositorInsertAplysia activating factor
UseTagsEGFPExpressionMutationPromoterAvailable sinceNov. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2
Plasmid#123618PurposeExpresses WT GgKDELR2 in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorInsertKDELR2 (KDELR2 Chicken)
UseTagsTEV-GFP-HisExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
px330-gp78
Plasmid#61833Purposeto make gp78 Knockout cell lineDepositorInsertgp78 (AMFR Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
YFP-Stim1-D76A
Plasmid#186622PurposeEncodes Stim1 mutant D76A fused to YFPDepositorInsertStromal Interaction Molecule 1 (STIM1 Human)
UseTagsYFPExpressionMammalianMutationD76APromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMTS-hFluc-HA-GFP11
Plasmid#177726PurposeFirefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting with an HA tag and the eleventh β-strand of GFP added to the C-terminusDepositorInserthFluc
UseTagsGFP11, HA, and Mitochondrial Targeting SequenceExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-ACBD5
Plasmid#171008PurposeHiLITR protease with ACBD5 C-terminal targeting information (peroxisome)DepositorInsertEGFP-uTEV1-ACBD5(tmd)
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV DCC mut
Plasmid#16460DepositorInsertDCC (DCC Human)
UseTagsExpressionMammalianMutation5' end of DCCPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
Frt-hspB10
Plasmid#63101PurposeExpression of HSPB10 (small HSP) in mammalian cellsDepositorInserthspb10 (ODF1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
PRK5-FLAG-USP13/5-4
Plasmid#61746Purposeexpress N-terminally FLAG tagged human chimera USP13/5-4DepositorUseTagsFLAGExpressionMammalianMutationUSP5 (1-716aa) fused to USP13 (722-864aa)PromoterCMVAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRK5-FLAG-USP13/5-3
Plasmid#61745Purposeexpress N-terminally FLAG tagged human chimera USP13/5-3DepositorUseTagsFLAGExpressionMammalianMutationUSP5 (1-628aa) fused to USP13 (653-864aa)PromoterCMVAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRK5-FLAG-USP13/5-1
Plasmid#61743Purposeexpress N-terminally FLAG tagged human chimera USP13/5-1DepositorUseTagsFLAGExpressionMammalianMutationUSP5 (1-185aa) fused to USP13 (197-864aa)PromoterCMVAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-KLF5-his
Plasmid#40903DepositorInsertKruppel-like factor 5 (KLF5 Human)
UseTagsHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo:alpha4-mcherry
Plasmid#45073DepositorInsertalpha4-mcherry (Chrna4 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pB-puro-mU6-CNPshRNA1
Plasmid#126611PurposeExpresses shRNA against human CNP under mouse U6 promoterDepositorInsertCNP ShRNA-1 (CNP Human)
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-shEsr1
Plasmid#120720PurposeLentiviral vector that expresses GFP and an shRNA targeting Esr1 (in pLL3.7).DepositorInsertEsr1 shRNA (Esr1 Mouse)
UseLentiviral and RNAiTagsGFPExpressionMammalianMutationPromoterMouse U6Available sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2alpha D291N mutant GFP
Plasmid#128821PurposeTo visualize Doc2alpha D291N mutant in PC12 cellsDepositorInsertDoc2alpha (Doc2a Rat)
UseTagsEGFPExpressionMammalianMutationD291NPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2beta D303N mutant GFP
Plasmid#128822PurposeTo visualize Doc2beta D303N mutant in PC12 cellsDepositorInsertDoc2beta (Doc2b Rat)
UseTagsEGFPExpressionMammalianMutationD303NPromoterEF-1alphaAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA 5/FR/TO-Ubl4A K48R
Plasmid#61811Purposeexpress C-terminal Ubl4A K48R, for making the stable cell lineDepositorInsertUBL4A (UBL4A Human)
UseTagsFLAGExpressionMammalianMutationK48RPromoterCMVAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG
Plasmid#200793PurposeMammalian expression of CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAGDepositorInsertKIF5A (Kif5a Mouse)
UseTagsCLIPf and SNAPf, HisTag, FLAGExpressionMammalianMutation1-914; Δ505-610PromoterCMV & T7Available sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL
Plasmid#216253PurposeFRET-based calcium sensor located in the ER lumen, Tet-inducibleDepositorInsertER-targeted D4 second generation cameleon (FRET calcium sensor)
UseLentiviralTagsREAch and mTurquoiseExpressionMutationPromoterTRE3GAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
FKBP-LBR(1-245)-GFP
Plasmid#222425PurposeTo express a truncated LBR (encoding amino acids 1-245) with N-terminal FKBP tag accessible to the cytoplasm and C-terminal GFP in mammalian cellsDepositorInsertLBR(1-245aa) (LBR Human)
UseTagsFKBP and GFPExpressionMammalianMutationTruncation (Includes aa 1-245)PromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast CG1358
Plasmid#218541PurposeDrosophila CG1358 cDNA (human codon optimized)DepositorInsertCG1358 (HisT Fly)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast SLC5A7
Plasmid#218539Purposehuman SLC5A7 cDNADepositorInsertSLC5A7 (SLC5A7 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast SLC44A1
Plasmid#218538Purposehuman SLC44A1 cDNADepositorInsertSLC44A1 (SLC44A1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBDP
Plasmid#217827PurposeStuffer plasmid with golden gate compatible bidirectional promoterDepositorInsertGal1/Gal10 Bidirectional Promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 (96-421)
Plasmid#213619PurposeBacterial expression of SUMO-tagged rat syt1 (96-421)DepositorInsertsyt1 (96-421) (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 (136-403)
Plasmid#213621PurposeBacterial expression of SUMO-tagged mouse syt7 (136-403)DepositorInsertsyt7 (136-403) (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 5W
Plasmid#213623PurposeBacterial expression of SUMO-tagged rat syt1 5WDepositorInsertsyt1 5W (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationM173W, F231W, F234W, C277A, V304W, Y364W, I367CPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 s7+
Plasmid#213624PurposeBacterial expression of SUMO-tagged rat syt1 s7+DepositorInsertsyt1 s7+ (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationL171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…PromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 C2AB - 4A
Plasmid#213625PurposeBacterial expression of SUMO-tagged mouse syt7 C2AB - 4ADepositorInsertsyt7 C2AB - 4A (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationF167A, F229A, I298A, L361APromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 C2AB-2A
Plasmid#213627PurposeBacterial expression of SUMO-tagged mouse syt7 C2AB-2ADepositorInsertsyt7 C2AB-2A (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationI298, L361APromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFSynW pHluorin-SYT9 IRES mRuby3
Plasmid#195698PurposeLentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoterDepositorInsertSyt9 (Syt5 Rat)
UseLentiviralTagspHluorinExpressionMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW Syt9 IRES mRuby3
Plasmid#195700PurposeLentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoterDepositorInsertSyt9 (Syt5 Mouse)
UseLentiviralTagsExpressionMutationencodes only the first 68 amino acids of Tac1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW SYT9 D197, 199N IRES GFP
Plasmid#195701PurposeLentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoterDepositorInsertSyt9 (Syt5 Mouse)
UseLentiviralTagsExpressionMutationfull-length mouse SYT9 with D197, 199N mutationsPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW SYT9 D330, 332N IRES GFP
Plasmid#195702PurposeLentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoterDepositorInsertSyt9 (Syt5 Mouse)
UseLentiviralTagsExpressionMutationfull-length mouse SYT9 with D330, 332N mutationsPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2AB D197,199,330,332N
Plasmid#195706PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2AB domain with D197, 199, 330, 332N mutationsDepositorInsertSyt9 (Syt5 Mouse)
UseTagsGSTExpressionBacterialMutationencodes amino acids 104-386 of mouse SYT9 with D1…PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-ST-P2A-pSC-CAAX
Plasmid#207637PurposeA plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.DepositorInsertEGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX
UseTagsExpressionMammalianMutationAmber stop codon at SC's critical lysinePromoterCMVAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC-cUnaG-LPETG
Plasmid#207635PurposeA plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.DepositorInsertphotocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG
UseTags6xHisExpressionBacterialMutationAmber stop codon at SC's critical lysinePromoterT7Available sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-Best1-Txnip(C247S_LL351-352AA)
Plasmid#206344PurposeAAV plasmid expressing mutant TXNIP in retinal pigment epitheliumDepositorInsertTxnip (Txnip Mouse)
UseAAVTagsExpressionMutationC247S, L351A, L352APromoterhuman Best1Available sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[B]_rescue_construct
Plasmid#190638PurposePuromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationChanged threonine 295 to methioninePromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[A]_rescue_construct
Plasmid#190640PurposePuromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFRTpsiCHECK_SEAP
Plasmid#154461PurposeExpression of SEAP and firefly luciferaseDepositorInsertSEAP
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
CTAG_eGFP-GHtag-HDEL
Plasmid#136096PurposeFP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tagDepositorInsertCTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJS697
Plasmid#168778PurposeYeast surface display plasmid backbone that can be combined with pJS699 through homologous recombinationDepositorTypeEmpty backboneUseTagsCmyc and HAExpressionYeastMutationPromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ad-HisFlag-USP19ER
Plasmid#155244Purposefor generating adenoviruses expressing the ER isoform of rat USP19DepositorInsertUSP19 (ER isoform) (Usp19 Rat)
UseAdenoviralTags6XHis-3XFlagExpressionMutationH118R- please see dep. commentsPromoterCMVAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisFlag-rUSP19ER-CAmut
Plasmid#155237Purposefor mammalian overexpression of the mutated ER isoform of rat USP19DepositorInsertUSP19 (ER isoform) (Usp19 Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationCys(545) to AlaPromoterCMVAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz26_CsoS2_N1-N2_GFP
Plasmid#140851PurposeHis-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backboneDepositorInsertCsoS2 NTD repeats 1-2 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-cytXlATL2-R232Q
Plasmid#124064PurposeControl protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATLDepositorInsertcytXlATL2-R232Q
UseTagsExpressionBacterialMutationPromotertacAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only