We narrowed to 3,024 results for: ER

1. pSLS.416

   Plasmid

   #187207

   Purpose

   Expresses retron Eco1 ncRNA v35 (long a1/a2), and Cas1 + Cas2

   Depositor

   Seth Shipman

   Article

   Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

   Inserts

   Retron Eco1 ncRNA v35 (long a1/a2)
   Cas1 + Cas2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7/Lac

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA 5/FR/TO-Ubl4A

   Plasmid

   #61810

   Purpose

   express C-terminal Ubl4A, for making the stable cell line

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   UBL4A (UBL4A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pFSW Doc2alpha WT

   Plasmid

   #128812

   Purpose

   To make virus expressing Doc2alpha WT

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2alpha (Doc2a Rat)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFSW Doc2beta WT

   Plasmid

   #128814

   Purpose

   To make virus expressing Doc2beta WT

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2beta (Doc2b Rat)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDDGFP-Leu2d-GgKDELR2

   Plasmid

   #123618

   Purpose

   Expresses WT GgKDELR2 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Use

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNEXdelta-EGFP-ApAF

   Plasmid

   #13335

   Depositor

   Bong-Kiun Kaang

   Article

   Lee et al J Cell Biol. 2006 Sep 11. 174(6):827-38.

   Insert

   Aplysia activating factor
   

   Use

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

7. px330-gp78

   Plasmid

   #61833

   Purpose

   to make gp78 Knockout cell line

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   gp78 (AMFR Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pMTS-hFluc-HA-GFP11

   Plasmid

   #177726

   Purpose

   Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting with an HA tag and the eleventh β-strand of GFP added to the C-terminus

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   hFluc
   

   Use

   Tags

   GFP11, HA, and Mitochondrial Targeting Sequence

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YFP-Stim1-D76A

   Plasmid

   #186622

   Purpose

   Encodes Stim1 mutant D76A fused to YFP

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Stromal Interaction Molecule 1 (STIM1 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   D76A

   Promoter

   CMV

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV DCC mut

    Plasmid

    #16460

    Depositor

    Bert Vogelstein

    Article

    Fearon et al Science. 1990 Jan 5. 247(4938):49-56.

    Insert

    DCC (DCC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5' end of DCC

    Promoter

    Available since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EGFP-uTEV1-ACBD5

    Plasmid

    #171008

    Purpose

    HiLITR protease with ACBD5 C-terminal targeting information (peroxisome)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-ACBD5(tmd)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Frt-hspB10

    Plasmid

    #63101

    Purpose

    Expression of HSPB10 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb10 (ODF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-KLF5-his

    Plasmid

    #40903

    Depositor

    Jin-Tang Dong

    Article

    Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

    Insert

    Kruppel-like factor 5 (KLF5 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pB-puro-mU6-CNPshRNA1

    Plasmid

    #126611

    Purpose

    Expresses shRNA against human CNP under mouse U6 promoter

    Depositor

    Xiaozhong Wang

    Article

    Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

    Insert

    CNP ShRNA-1 (CNP Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    mouse U6 promoter

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV-shEsr1

    Plasmid

    #120720

    Purpose

    Lentiviral vector that expresses GFP and an shRNA targeting Esr1 (in pLL3.7).

    Depositor

    Amy Lasek

    Article

    Satta et al Int J Neuropsychopharmacol. 2018 Apr 1;2

    Insert

    Esr1 shRNA (Esr1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse U6

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEF Doc2alpha D291N mutant GFP

    Plasmid

    #128821

    Purpose

    To visualize Doc2alpha D291N mutant in PC12 cells

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2alpha (Doc2a Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D291N

    Promoter

    EF-1alpha

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEF Doc2beta D303N mutant GFP

    Plasmid

    #128822

    Purpose

    To visualize Doc2beta D303N mutant in PC12 cells

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2beta (Doc2b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D303N

    Promoter

    EF-1alpha

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. IRS1-B1

    Plasmid

    #25698

    Depositor

    Eric Fearon

    Article

    Bommer et al J Biol Chem. 2010 Jan 15. 285(3):1928-38

    Insert

    IRS1 (IRS1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

19. IRS1-A4

    Plasmid

    #25697

    Depositor

    Eric Fearon

    Article

    Bommer et al J Biol Chem. 2010 Jan 15. 285(3):1928-38

    Insert

    IRS1 (IRS1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA 5/FR/TO-Ubl4A K48R

    Plasmid

    #61811

    Purpose

    express C-terminal Ubl4A K48R, for making the stable cell line

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    UBL4A (UBL4A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K48R

    Promoter

    CMV

    Available since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

21. CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

    Plasmid

    #200793

    Purpose

    Mammalian expression of CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

    Depositor

    Alison Twelvetrees

    Article

    Smith et al bioRxiv 2024.12.20.629623

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    CLIPf and SNAPf, HisTag, FLAG

    Expression

    Mammalian

    Mutation

    1-914; Δ505-610

    Promoter

    CMV & T7

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Stargazin-EBFP2-FRB

    Plasmid

    #222417

    Purpose

    To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    Cacng2 (Cacng2 Mouse)
    

    Use

    Tags

    EBFP2-HA-FRB

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GFP-FKBP-LBR

    Plasmid

    #222427

    Purpose

    To express LBR with N-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    LBR (LBR Human)
    

    Use

    Tags

    GFP-FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMXS-IRES-Blast CG1358

    Plasmid

    #218541

    Purpose

    Drosophila CG1358 cDNA (human codon optimized)

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    CG1358 (HisT Fly)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBDP

    Plasmid

    #217827

    Purpose

    Stuffer plasmid with golden gate compatible bidirectional promoter

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Insert

    Gal1/Gal10 Bidirectional Promoter
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET SUMO-syt1 (96-421)

    Plasmid

    #213619

    Purpose

    Bacterial expression of SUMO-tagged rat syt1 (96-421)

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt1 (96-421) (Syt1 Rat)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET SUMO-syt7 (136-403)

    Plasmid

    #213621

    Purpose

    Bacterial expression of SUMO-tagged mouse syt7 (136-403)

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt7 (136-403) (Syt7 Mouse)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET SUMO-syt1 5W

    Plasmid

    #213623

    Purpose

    Bacterial expression of SUMO-tagged rat syt1 5W

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt1 5W (Syt1 Rat)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    M173W, F231W, F234W, C277A, V304W, Y364W, I367C

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET SUMO-syt1 s7+

    Plasmid

    #213624

    Purpose

    Bacterial expression of SUMO-tagged rat syt1 s7+

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt1 s7+ (Syt1 Rat)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    L171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET SUMO-syt7 C2AB - 4A

    Plasmid

    #213625

    Purpose

    Bacterial expression of SUMO-tagged mouse syt7 C2AB - 4A

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt7 C2AB - 4A (Syt7 Mouse)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    F167A, F229A, I298A, L361A

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET SUMO-syt7 C2AB-2A

    Plasmid

    #213627

    Purpose

    Bacterial expression of SUMO-tagged mouse syt7 C2AB-2A

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    syt7 C2AB-2A (Syt7 Mouse)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    I298, L361A

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFSynW pHluorin-SYT9 IRES mRuby3

    Plasmid

    #195698

    Purpose

    Lentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Rat)
    

    Use

    Lentiviral

    Tags

    pHluorin

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFSynW Syt9 IRES mRuby3

    Plasmid

    #195700

    Purpose

    Lentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    encodes only the first 68 amino acids of Tac1

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFSynW SYT9 D197, 199N IRES GFP

    Plasmid

    #195701

    Purpose

    Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    full-length mouse SYT9 with D197, 199N mutations

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFSynW SYT9 D330, 332N IRES GFP

    Plasmid

    #195702

    Purpose

    Lentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    full-length mouse SYT9 with D330, 332N mutations

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGEX SYT9 C2AB D197,199,330,332N

    Plasmid

    #195706

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain with D197, 199, 330, 332N mutations

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 104-386 of mouse SYT9 with D1…

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. EGFP-ST-P2A-pSC-CAAX

    Plasmid

    #207637

    Purpose

    A plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    EGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    CMV

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSC-cUnaG-LPETG

    Plasmid

    #207635

    Purpose

    A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    photocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    T7

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AAV-Best1-Txnip(C247S_LL351-352AA)

    Plasmid

    #206344

    Purpose

    AAV plasmid expressing mutant TXNIP in retinal pigment epithelium

    Depositor

    Connie Cepko

    Article

    Xue et al Elife. 2021 Apr 13;10. pii: 66240. doi:

    Insert

    Txnip (Txnip Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    C247S, L351A, L352A

    Promoter

    human Best1

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. HA-dora[B]_rescue_construct

    Plasmid

    #190638

    Purpose

    Puromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    3xHA

    Expression

    Insect

    Mutation

    Changed threonine 295 to methionine

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. HA-dora[A]_rescue_construct

    Plasmid

    #190640

    Purpose

    Puromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    3xHA

    Expression

    Insect

    Mutation

    Truncated to contain amino acids 1-945

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pFRTpsiCHECK_SEAP

    Plasmid

    #154461

    Purpose

    Expression of SEAP and firefly luciferase

    Depositor

    Markus Landthaler

    Article

    Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

    Insert

    SEAP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. CTAG_eGFP-GHtag-HDEL

    Plasmid

    #136096

    Purpose

    FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tag

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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44. pJS697

    Plasmid

    #168778

    Purpose

    Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Type

    Empty backbone

    Use

    Tags

    Cmyc and HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. Ad-HisFlag-USP19ER

    Plasmid

    #155244

    Purpose

    for generating adenoviruses expressing the ER isoform of rat USP19

    Depositor

    Simon Wing

    Article

    Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

    Insert

    USP19 (ER isoform) (Usp19 Rat)
    

    Use

    Adenoviral

    Tags

    6XHis-3XFlag

    Expression

    Mutation

    H118R- please see dep. comments

    Promoter

    CMV

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. pcDNA-HisFlag-rUSP19ER-CAmut

    Plasmid

    #155237

    Purpose

    for mammalian overexpression of the mutated ER isoform of rat USP19

    Depositor

    Simon Wing

    Article

    Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

    Insert

    USP19 (ER isoform) (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Cys(545) to Ala

    Promoter

    CMV

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLz26_CsoS2_N1-N2_GFP

    Plasmid

    #140851

    Purpose

    His-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeats 1-2 fused to GFP
    

    Use

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pGEX-4T-3-cytXlATL2-R232Q

    Plasmid

    #124064

    Purpose

    Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

    Depositor

    Tom Rapoport

    Article

    Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

    Insert

    cytXlATL2-R232Q
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3

    Plasmid

    #111923

    Purpose

    Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_19μ
    

    Use

    Tags

    CoxVIIIx2

    Expression

    Mammalian

    Mutation

    E67D, F92W, E104D, D133E at CaM

    Promoter

    CMV

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. CoxVIIIx2-CeNL(Ca2+)_1.2μ-pcDNA3

    Plasmid

    #111922

    Purpose

    Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_1.2μ
    

    Use

    Tags

    CoxVIIIx2

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge